Biofunctionalized cellulose paper matrix for cell delivery applications

Agarwal, Tarun; Maiti, Tapas K.; Behera, Birendra; Ghosh, Sudip Kumar; Apoorva, Anupam; Manchikanti, Padmavati

doi:10.1016/j.ijbiomac.2019.07.201

Cited by 13 publications

(17 citation statements)

References 55 publications

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“…Prior to imaging, the devices were sputter-coated with gold and then visualized at 20 kV voltage. The culturable zones were further biofunctionalized with Caprine liver-derived extracellular matrix (CLECM) (200 μg/mL, following either direct coating or APTES-mediated coating methodology as reported elsewhere , ). In our work, we fabricated three different formats of the devices intended for a specific application: Design 1, for routine cell culture, immunocytochemistry, in-cell ELISA, drug screening, and coculture assays; Design 2, for optimization assays; and Design 3, for transwell migration assay (Figure A).…”

Section: Methodsmentioning

confidence: 99%

“…In this regard, the paper has come up as a promising alternative. The paper consists of randomly three-dimensionally arranged cellulose fibrils to form a porous matrix of <200 μm thickness. , Moreover, the features such as biocompatibility, cost-effectiveness, easy availability, passive mass-transport (via capillary action in the micron level dimensionality), suitability to chemical and physical modifications, and flexible nature have led to its vast exploration in the field of biomedical engineering. , Over the past few years, the paper matrix has been for the development of various cell-based assays including in-cell ELISA, , cell migration/invasion assay, − drug screening, − cellular cross-talk, and solute release quantification . To the best of our knowledge, there exist only a few studies pertaining to the culture of liver cells on the paper, ,,, and only a single study that addresses the impact of 3D paper matrix on the hepatocyte functionality with an aim toward the development of the functional liver tissue coculture model on paper chip .…”

Section: Introductionmentioning

confidence: 99%

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Inexpensive and Versatile Paper-Based Platform for 3D Culture of Liver Cells and Related Bioassays

Agarwal

Biswas

Pal

et al. 2020

ACS Appl. Bio Mater.

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The present study delineates the fabrication of paper-based devices for culturing liver cells and developing related bioassays. The devices were prepared by conventional lab-based LaserJet printing technology and employed for 3D cell culture. Our results demonstrated that the devices efficiently supported the growth of multiple cell types incuding HepG2, HUVEC, fibroblasts, and MSCs. We further showed that the device specifications (grade of paper or design parameters) greatly impacted the functional phenotype of the HepG2 cells. We also explored the application of the developed devices for routine cell culture, drug screening, coculture, and transwell migration assays. The cellular responses observed on the paper under different culture configurations were similar to those obtained in the case of tissue culture plate (TCP). Moreover, we showed that the paper-based devices were compatible with the immunocytochemistry and ELISA procedures (no indication of nonspecific matrix-antibody interaction). Considering the simplicity, experimental flexibility, cost-effectiveness, and multiplexibility of the paper-based liver models, it is deemed to be ideal for developing cell-based bioassays, especially in resource-limited settings.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inexpensive and Versatile Paper-Based Platform for 3D Culture of Liver Cells and Related Bioassays

Agarwal

Biswas

Pal

et al. 2020

ACS Appl. Bio Mater.

Self Cite

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“…The functionalization of this system with photoluminescent 2-amino-2-methyl-1-propanol quantum carbon dots showed a limit of detection of 1.2 μM Cr 6+ , below the maximum allowable limit for drinking water. Recently, it was shown that functional amino-modification of cellulose-based paper scaffolds with either PAMAM (Agarwal et al 2018) or APTES (Agarwal et al 2019(Agarwal et al , 2020a, significantly improved the adhesion and spreading of the human hepatocellular carcinoma cells, HepG2. Interestingly, the degree of functionalization critically modulated the cytotoxicity and adhesion properties of the scaffolds.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Functionalization of polymers and nanomaterials for water treatment, food packaging, textile and biomedical applications: a review

et al. 2020

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The inert nature of most commercial polymers and nanomaterials results in limitations of applications in various industrial fields. This can be solved by surface modifications to improve physicochemical and biological properties, such as adhesion, printability, wetting and biocompatibility. Polymer functionalization allows to graft specific moieties and conjugate molecules that improve material performances. In the last decades, several approaches have been designed in the industry and academia to graft functional groups on surfaces. Here, we review surface decoration of polymers and nanomaterials, with focus on major industrial applications in the medical field, textile industry, water treatment and food packaging. We discuss the advantages and challenges of polymer functionalization. More knowledge is needed on the biology behind cell–polymer interactions, nanosafety and manufacturing at the industrial scale.

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“…However, these scaffolds could not provide cells with all the bioactive molecules required for their growth as compared with decellularized ECM, which is why synthetic materials were mixed with ECM powder or solubilized ECM to make them more biocompatible and functional. However, many factors regarding the physical and mechanical properties of the synthetic scaffolds should be optimized in order to be approved for clinical application [110,123].…”

Section: Bioengineered Liver Scaffolds and Their Transplantationmentioning

confidence: 99%

Cell Therapy and Bioengineering in Experimental Liver Regenerative Medicine: In Vivo Injury Models and Grafting Strategies

et al. 2021

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Purpose of Review To describe experimental liver injury models used in regenerative medicine, cell therapy strategies to repopulate damaged livers and the efficacy of liver bioengineering. Recent Findings Several animal models have been developed to study different liver conditions. Multiple strategies and modified protocols of cell delivery have been also reported. Furthermore, using bioengineered liver scaffolds has shown promising results that could help in generating a highly functional cell delivery system and/or a whole transplantable liver. Summary To optimize the most effective strategies for liver cell therapy, further studies are required to compare among the performed strategies in the literature and/or innovate a novel modifying technique to overcome the potential limitations. Coating of cells with polymers, decellularized scaffolds, or microbeads could be the most appropriate solution to improve cellular efficacy. Besides, overcoming the problems of liver bioengineering may offer a radical treatment for end-stage liver diseases.

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Biofunctionalized cellulose paper matrix for cell delivery applications

Cited by 13 publications

References 55 publications

Inexpensive and Versatile Paper-Based Platform for 3D Culture of Liver Cells and Related Bioassays

Inexpensive and Versatile Paper-Based Platform for 3D Culture of Liver Cells and Related Bioassays

Functionalization of polymers and nanomaterials for water treatment, food packaging, textile and biomedical applications: a review

Cell Therapy and Bioengineering in Experimental Liver Regenerative Medicine: In Vivo Injury Models and Grafting Strategies

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