Biofunctionalizing nanofibers with carbohydrate blood group antigens

Abstract

A rapid and simple method of biofunctionalising nylon, cellulose acetate, and polyvinyl butyral electrospun nanofibers with blood group glycans was achieved by preparing function-spacer-lipid constructs and simply contacting them to fibers with a piezo inkjet printer. A series of water dispersible amphipathic glycan-spacer constructs were synthesized representing a range ABO and related blood group antigens. After immediate contact of the amphipathic glycan-spacer constructs with nanofiber surfaces they self-assembled and were detectable by enzyme immunoassays with high sensitivity and specificity.

Ultra-Fast Glyco-Coating of Non-Biological Surfaces

“…The ability of the FSL constructs to self-assemble on nanofibres during the electrospinning process was established by adding the Atri-Ad-DOPE construct to two different nanofiber polymers (CA and PVB) before electrospinning of the fibres [ 11 ]. The resulting fibres when stained with the EIA technique shows incorporation of the FSLs into the fibres, and presence of the immunoreactive antigen at the nanofibre surface ( Figure 7 ).…”

“…Nanofibres incorporating FSL constructs were prepared using an in house apparatus at the premises of Revolution Fibres Ltd., Auckland, New Zealand [ 11 ]. Dispersions of polyvinyl butyral (PVB) were prepared at a concentration of 10% ( w / v ) in 100% ethanol.…”

“…Each surface or prepared microplate well was flooded or filled with IgM monoclonal anti-A (Epiclone anti-A, Cat# 0261, CSL, Seaford, VIC, Australia) diluted 1:4 with BSA-PBS and then incubated for 30 min at RT. This anti-A reagent has been previously established to show specific binding against the blood group A antigen, including in the form of FSL constructs [ 4 , 5 , 7 , 8 , 10 , 11 ]. Unprinted areas within the assay are internal blanks.…”