Biogas production and microbial community shift through neutral pH control during the anaerobic digestion of pig manure

Zhou, Jun; Zhang, Rui; Liu, Fen­wu; Yong, Xiaoyu; Wu, Xuezhong; Zheng, Tao; Jiang, Min; Jia, Honghua

doi:10.1016/j.biortech.2016.02.077

Cited by 121 publications

(44 citation statements)

References 31 publications

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“…Results from this work agrees with [16] who found out that CH 4 content significantly increase to 74% under alkaline condition as compared to other conditions. …”

Section: Methane Productionsupporting

confidence: 92%

Optimization of Biogas Production in Dry Anaerobic Digestion of Swine Manure by the Use of Alkalinity Index to Monitor a Prototype Cylindrical Digester

Andole¹,

Lei²,

Zhang³

et al. 2017

RSE

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Anaerobic digestion (AD) is one of the best alternative sustainable technologies for energy production and recovery from organic solid wastes. Up to now dry AD has been commercialized in the treatment of municipal solid wastes. Little information could be found on the practical application of dry AD to manure wastes or waste activated sludge. This study aimed at testing the feasibility of using alkalinity to manage dry AD system for swine manure treatment and clarify its effect on the stability and efficiency of the newly-developed prototype cylindrical digester system. A prototype cylindrical digester with a diameter of 40 mm and a volume of 1.3 liters was designed and fabricated. It was operated under mesophilic conditions (38°C). The alkalinity of manure was increased by 3000 g/L (R 1 ) and 6000 g/L (R 2 ) by adding sodium bicarbonate with the raw swine manure as the control (R 0 ). Results showed that R 1 and R 2 maintained a relatively higher level of alkalinity during the whole operation compared to the control (R 0 ). Only one peak appeared in biogas production for the control reactor (R 0 ) which almost ceased on day 12, whereas R 1 and R 2 exhibited two biogas peaks. The 30 days' biogas yield for R 2 was 276.6 ml/g-VS added while R 1 was 204.8 ml/g-VS added which corresponds to an increase by 2.7-and 1.7-fold respectively as compared to the control (R 0 ). 2.2-and 4.1-fold increase in methane production was achieved in R 1 and R 2 respectively as compared to R 0 . This difference is most probably attributable to the high alkalinity in R 1 and R 2 that stabilized the digestion process and minimized the influence of pH variations on methanogenesis.

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“…Results from this work agrees with [16] who found out that CH 4 content significantly increase to 74% under alkaline condition as compared to other conditions. …”

Section: Methane Productionsupporting

confidence: 92%

Optimization of Biogas Production in Dry Anaerobic Digestion of Swine Manure by the Use of Alkalinity Index to Monitor a Prototype Cylindrical Digester

Andole¹,

Lei²,

Zhang³

et al. 2017

RSE

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“…From an energy-efficient point of view, the anaerobic digestion of pig slurry is much more adequate. When the slurry decomposes in the absence of oxygen it produces methane, a renewable fuel, contributing to environmental protection and energy demand reduction (Zhou et al, 2016). This option, instead of consuming energy, produces it and the resulting energy can be used by the farms themselves, while the digested slurry is usable as fertilizer with improved properties (Antezana et al, 2016;Massé et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of different types of anaerobic seed sludge for the high rate anaerobic digestion of pig slurry in UASB reactors

Rico

Montes

Rico

2017

Bioresource Technology

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Three different types of anaerobic sludge (granular, thickened digestate and anaerobic sewage) were evaluated as seed inoculum sources for the high rate anaerobic digestion of pig slurry in UASB reactors. Granular sludge performance was optimal, allowing a high efficiency process yielding a volumetric methane production rate of 4.1LCHLd at 1.5days HRT (0.248LCHgCOD) at an organic loading rate of 16.4gCODLd. The thickened digestate sludge experimented flotation problems, thus resulting inappropriate for the UASB process. The anaerobic sewage sludge reactor experimented biomass wash-out, but allowed high process efficiency operation at 3days HRT, yielding a volumetric methane production rate of 1.7LCHLd (0.236LCHgCOD) at an organic loading rate of 7.2gCODLd. To guarantee the success of the UASB process, the settleable solids of the slurry must be previously removed.

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“…These studies have shown that each type of bioreactor harbors a specific microbial community (Sundberg et al, 2013;Abendroth et al, 2015). Each particular community is determined by parameters such as the type of feedstock (Sundberg et al, 2013), temperature (Banach et al, 2018), retention time (Gaby et al, 2017), salt content (Wang et al, 2017;De Vrieze et al, 2017), viscosity (Hardegen et al, 2018), pH (Zhou et al, 2016), or the loading rate (Ciotola et al, 2014). Although the influence of many physicochemical parameters on microbial communities has been studied in anaerobic digesters, there is, to the best of our knowledge, no previous reports characterising the…”

Section: Introductionmentioning

confidence: 99%

Shedding light on biogas: a transparent reactor triggers the development of a biofilm dominated by Rhodopseudomonas faecalis that holds potential for improved biogas production

Abendroth

A²,

Porcar³

et al. 2019

Preprint

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Conventional anaerobic digesters intended for the production of biogas usually operate in complete darkness. Therefore, little is known about the effect of light on microbial communities operating in anaerobic digesters. In the present work, we have studied through 16S rRNA gene amplicon Nanopore sequencing and shotgun metagenomic sequencing the taxonomic and functional structure of the microbial community forming a biofilm on the inner wall of a lab-scale transparent anaerobic biodigester illuminated with natural sunlight. The biofilm was composed of microorganisms involved in the four metabolic processes needed for biogas production. The biofilm proved surprisingly rich in Rhodopseudomonas faecalis, a 2 4 6 8 10 12 14 16 18 20 22 24 26 28versatile bacterium able to carry out a photoautotroph metabolism when grown under anaerobic conditions. Our results suggest that this bacterium, able to fix carbon dioxide, could be considered for its use in transparent biogas fermenters in order to contribute to the production of optimized biogas with a higher CH4:CO2 ratio than the biogas produced in regular, opaque digesters. To the best of our knowledge, this is the first study supporting illuminated bioreactors as a new bioprocess for the obtention of biogas enriched in methane. Keywords: Anaerobic digestion, Rhodopseudomonas faecalis, optimized biogas, shotgun metagenomic sequencing, waste water treatment, phototrophism, 16S rRNA gene amplicon Nanopore sequencing.30 32 34 36 38 40The microbial communities operating in the digester are the final key players responsible for the quality of the produced biogas. The role of different microorganisms in the four metabolic steps carried out during the AD of organic matter (hydrolysis, fermentation, acetogenesis, and methanogenesis) has been widely studied (Zinder, 1984). A diverse number of Bacteria are known to be involved in the hydrolysis and further fermentation of complex polymers, whereas the oxidation of intermediate fermentation products to acetate is performed by either hydrogen-or formate-producing acetogens (Stams & Plugge, 2009). Lastly, methane synthesis is mainly derived from acetate and H2/CO2 by acetoclastic and hydrogenoclastic methanogenic Archaea. Therefore, an improved understanding of the microbial communities 42 44 46 48 50 52 54 56 58 60 62 64 influence of light in the process (Sawayama, 2000; Tada et al., 2006; Wei et al., 2016;Reverso, 2017), mainly because the obvious fact that conventional AD systems operate in complete darkness. Interestingly, a previous study reported an increment of the relative concentration of methane when an anaerobic digester was operated under the influence of light (Tada et al., 2006). However, a holistic study of the effect of light on the entire microbiome of anaerobic digesters had yet to be addressed.The aim of the present work was to analyse the effect of natural sunlight on the microbial community of a lab-scale anaerobic co-digester, in order to explore the potential of this strategy to produce high quality biogas. In o...

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Biogas production and microbial community shift through neutral pH control during the anaerobic digestion of pig manure

Cited by 121 publications

References 31 publications

Optimization of Biogas Production in Dry Anaerobic Digestion of Swine Manure by the Use of Alkalinity Index to Monitor a Prototype Cylindrical Digester

Optimization of Biogas Production in Dry Anaerobic Digestion of Swine Manure by the Use of Alkalinity Index to Monitor a Prototype Cylindrical Digester

Evaluation of different types of anaerobic seed sludge for the high rate anaerobic digestion of pig slurry in UASB reactors

Shedding light on biogas: a transparent reactor triggers the development of a biofilm dominated by Rhodopseudomonas faecalis that holds potential for improved biogas production

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