Biogas production through syntrophic acetate oxidation and deliberate operating strategies for improved digester performance

Westerholm, Maria; Moestedt, Jan; Schnürer, Anna

doi:10.1016/j.apenergy.2016.06.061

Cited by 267 publications

(230 citation statements)

References 119 publications

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“…This last step is accomplished by archaeal members of the community that use one or more of the three methanogenic pathways: the hydrogenotrophic pathway (H 2 + CO 2 → CH 4 ), the acetoclastic pathway (acetate → CH 4 ), and the less common methylotrophic pathway (one-carbon compounds such as methanol → CH 4 ). Anaerobic digestion of protein-rich substrates has a distinctly high methane production potential but frequently leads to accumulation of the intermediate metabolite ammonia, which can detrimentally affect the viability and metabolism of individual organisms, as well as the stability of the entire reactor microbiome (1–4). Reports have demonstrated that ammonia levels of >0.08 g/liter disturb the microbiome structure and biogas process, reflected by the accumulation of acetate and reduced methane production (1, 4).…”

Section: Introductionmentioning

confidence: 99%

Novel Syntrophic Populations Dominate an Ammonia-Tolerant Methanogenic Microbiome

et al. 2016

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The microbial production of methane or “biogas” is an attractive renewable energy technology that can recycle organic waste into biofuel. Biogas reactors operating with protein-rich substrates such as household municipal or agricultural wastes have significant industrial and societal value; however, they are highly unstable and frequently collapse due to the accumulation of ammonia. We report the discovery of a novel uncultured phylotype (unFirm_1) that is highly detectable in metaproteomic data generated from an ammonia-tolerant commercial reactor. Importantly, unFirm_1 is proposed to perform a key metabolic step in biogas microbiomes, whereby it syntrophically oxidizes acetate to hydrogen and carbon dioxide, which methanogens then covert to methane. Only very few culturable syntrophic acetate-oxidizing bacteria have been described, and all were detected at low in situ levels compared to unFirm_1. Broader comparisons produced the hypothesis that unFirm_1 is a key mediator toward the successful long-term stable operation of biogas production using protein-rich substrates.

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Section: Introductionmentioning

confidence: 99%

Novel Syntrophic Populations Dominate an Ammonia-Tolerant Methanogenic Microbiome

et al. 2016

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“…Enriched ammonia-tolerant methanogenic cultures have been used before as bioaugmentation inocula in batch reactors with high ammonia levels (Wilson et al, 2013). However, these enriched cultures were never assessed as bioaugmentation inocula in continuous anaerobic reactors where the washout of the inoculated microorganisms poses a big challenge (Han et al, 2016;Sivagurunathan et al, 2015;Westerholm et al, 2016).…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Effects of added enzymes on sorted, unsorted and sorted-out barley: A model study on realtime viscosity and process potentials using rapid visco analyser

Shetty

Zhuang

Olsen

et al. 2017

Journal of Cereal Science

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Many ammonia-rich biomass sources, such as manures and protein-rich substrates, are potential inhibitors of the anaerobic digestion (AD) process. It was previously demonstrated that bioaugmentation of Methanoculleus bourgensis MS2T in an ammonia inhibited process in a continuous stirred tank reactor (CSTR), resulted in up to 90 % recovery of the methane production compared to the uninhibited production. However, cultivation of pure strains has practical difficulties due to the need of special growth media and sterile conditions. In contrast, acclimatized enriched cultures have minor sterility requirements. In the current study, an enriched ammonia-tolerant methanogenic culture was bioaugmented in a CSTR reactor operating under ammonia-induced, inhibited-steady-state. The results demonstrated that bioaugmentation, completely counteracted the ammonia toxicity effect. This indicates that a commercial application of bioaugmentation could improve up to 36 % the methane production, the greenhouse gas reduction efficiency and the gross revenue of ammonia inhibited full scale biogas reactors. 16S rRNA gene sequencing showed that bioaugmentation changed the microbial composition of the reactors resulting in higher bacterial and lower archaeal community diversity. The bioaugmented reactor showed a fourfold increase of the abundance of the bioaugmented methanogens compared to the control reactor. This indicates that ammonia-tolerant methanogens established well in the ammonia-inhibited reactor and dominated over the domestic methanogenic population. Finally, this study showed that the enriched culture alleviated ammonia toxicity 25 % more efficiently than the previously used pure culture.

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“…Syntrophic acetate oxidation is essential for the biomethanisation of organic matter in engineered anaerobic digestion (AD) processes, in particular those characterised by high ammonia levels [1][2][3]. Engineered biogas processes are being increasingly used in Europe and worldwide due to its potential to meet environmental and climate-related targets and to secure future energy supplies by reducing the exploitation of finite resources and recycling nutrients and energy.…”

Section: Introductionmentioning

confidence: 99%

“…Using organic wastes produced by agriculture, municipalities and the food industry as substrates in the biogas process achieves sustainable and economical recycling of nutrients and energy between urban and rural areas, due the multi-functionality of the process, and reduces competition for land between food and energy production. However, use of highenergy biomass materials such as industrial food waste is still challenging, as degradation of proteinaceous materials releases high levels of ammonia, which has a direct impact on the prevailing methane production pathway, with serious consequences for process stability and efficiency [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Genome-Guided Analysis of the Syntrophic Acetate Oxidizer <em>C. ultunense</em> and Comparative Genomics Reveal Different Strategies for Acetate Oxidation and Energy Conservation in Syntrophic Acetate-Oxidising Bacteria

Manzoor¹,

Schnürer²,

Bongcam‐Rudloff³

et al. 2018

Preprint

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ABSTRACTschinkii, were identified in C. ultunense and P. lettingae. Moreover, two respiratory complexes sharing similarities to the proton-translocating ferredoxin:NAD + oxidoreductase (Rnf) and the Na+ pumping NADH:quinone hydrogenase (NQR) were predicted. These might form a respiratory chain involved in reduction of electron acceptors other than protons.However, involvement of these complexes in acetate oxidation in C. ultunense and P.lettingae needs further study. This genome-based comparison provides a solid platform for future meta-proteomics and meta-transcriptomics studies and for metabolic engineering, control and monitoring of SAOB.

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Biogas production through syntrophic acetate oxidation and deliberate operating strategies for improved digester performance

Cited by 267 publications

References 119 publications

Novel Syntrophic Populations Dominate an Ammonia-Tolerant Methanogenic Microbiome

Novel Syntrophic Populations Dominate an Ammonia-Tolerant Methanogenic Microbiome

Effects of added enzymes on sorted, unsorted and sorted-out barley: A model study on realtime viscosity and process potentials using rapid visco analyser

Genome-Guided Analysis of the Syntrophic Acetate Oxidizer <em>C. ultunense</em> and Comparative Genomics Reveal Different Strategies for Acetate Oxidation and Energy Conservation in Syntrophic Acetate-Oxidising Bacteria

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