“…Siddiqi et al (2022) also detected a reduction in C, H, and N contents, as well as extraction of some minerals, due to wheat straw pretreatment with NaOH. As a result of the loss of these elements, the contents of other elements, such as Ca, Cu, Ni, Fe, Zn, Mn, Co, Mo, and S, increased, as reported previously (Jaffar et al 2016;Hassan et al 2017;Al-Da'asen et al 2022;Rani et al 2022). Moreover, a statistical analysis showed a significant difference between the T3 and T0 values for most parameters, and straw pretreatment with NaOH (T3) was associated with the greatest reductions in the reducing elements, followed by those of KOH (T2) and Na2CO3 (T1).…”