Biogeneration of ethyl valerate by whole cells of Pseudomonas fragi CRDA 037 in aqueous medium

Lamer, Sophie Le; Leblanc, Danielle; Morin, André; Kermasha, Sélim

doi:10.1007/bf00159508

Cited by 10 publications

(6 citation statements)

References 8 publications

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“…fragi (Morin et al, 1994;Leblanc et al, 1998) and its production from ethanol and valeric acid is influenced by pH, temperature, substrate concentration and ratio, and cell biomass (Lamer, Leblanc, Morin, & Kermasha, 1996). Indeed, Ps.…”

Section: Ester Biosynthesis By Dairy Pseudomonadsmentioning

confidence: 99%

Esters and their biosynthesis in fermented dairy products: a review

Liu

Holland

Crow

2004

International Dairy Journal

338

269

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Section: Ester Biosynthesis By Dairy Pseudomonadsmentioning

confidence: 99%

Esters and their biosynthesis in fermented dairy products: a review

Liu

Holland

Crow

2004

International Dairy Journal

338

269

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“…Biotransformations were performed in Dreschel gas washing bottles coupled with a sintered glass, porosity no 3 (Quickfit from Pegasus, Agincourt, Ontario, Canada) according to the procedures of Lamer et al 12 The reaction mixture was composed of 10 mol m −3 butyric acid, 20 mol m −3 food grade ethyl alcohol and 10% (v/v) of the 25% cell suspension in 100 mol m −3 Tris–HCl buffer, pH 9.0.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Waters Silica Sep-Pak cartridges for short chain fatty acid ethyl ester recovery

Leblanc

Morin

Houde

2000

J. Chem. Technol. Biotechnol.

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Waters Silica Sep-Pak cartridges were evaluated for their performance in trapping short chain fatty acid ethyl esters. Liquid solutions of ethyl butyrate, ethyl valerate and ethyl caproate were injected onto cartridges. All the esters bound to the matrix and were eluted with four bed volumes of ethanol for ethyl butyrate and two bed volumes of ethanol for ethyl valerate and ethyl caproate. Samples of esters stripped with an air stream for 1 min and trapped on cartridges were found to vary by less than 8% for ethyl propionate, ethyl butyrate, ethyl valerate and ethyl caproate. Production of ethyl butyrate by Pseudomonas fragi CRDA 037 resting cells during biotransformation was ef®ciently evaluated using these cartridges. Variations between samples were found to be less than 10%.

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“…The esterifying activity was assayed in 250-ml Dreschel gas washing bottles outfitted with a sintered-glass gas inlet (porosity no. 3) and a gas outlet at 12°C for 6 h as described by Lamer et al (9). The reaction mixture consisted of 90 ml of Tris-HCl buffer (0.1 M, pH 9.0), 10 ml of 25% cell suspension, 0.01 M valeric acid (Laboratoire Mat, Beauport, Quebec, Canada), and 0.02 M food-grade ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…For every assay of esterifying activity, three traps were collected and analyzed. The aroma compounds were desorbed from the Tenax traps by using a thermal AERO Trap Desorber 6000 (Tekmar, Cincinnati, Ohio) and injected into a Perkin-Elmer model 8320 gas chromatograph operating with a flame ionization detector under the conditions reported by Lamer et al (9). The gas chromatograph analysis gave the amount of ethyl valerate in micrograms produced per 2 min.…”

Section: Methodsmentioning

confidence: 99%

Effect of Growth Temperature on Hydrolytic and Esterifying Activities from Pseudomonas fragi CRDA 037 Grown on Whey

Fonchy

Morin

Rodrigue

et al. 1999

Appl Environ Microbiol

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The production of hydrolytic and esterifying activities ofPseudomonas fragi CRDA 037 grown on a whey-based medium was investigated at different temperatures over time. The optimal temperature was found to be critical and different for the production of both activities. The highest hydrolytic activity was detected with bacteria cultivated at between 24°C (149.2 U/liter) and 27°C (133.8 U/liter), while the highest production of ethyl valerate (esterifying activity) was observed by using biomass grown at 15°C (0.75 U/liter). When the fermentation temperature was increased, the incubation time necessary to reach the maximal concentration of both activities was reduced. Studies of the thermostability of both activities showed that the hydrolytic activity was more stable than the esterifying activity at 15 and 24°C. Statistical analysis allowed the determination of the equations that predicted the production of hydrolytic and esterifying activities as a function of time and growth temperature. The optimal assay temperatures for the hydrolytic and esterifying activities were 37°C and 12 to 15°C, respectively.

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Biogeneration of ethyl valerate by whole cells of Pseudomonas fragi CRDA 037 in aqueous medium

Cited by 10 publications

References 8 publications

Esters and their biosynthesis in fermented dairy products: a review

Esters and their biosynthesis in fermented dairy products: a review

Evaluation of Waters Silica Sep-Pak cartridges for short chain fatty acid ethyl ester recovery

Effect of Growth Temperature on Hydrolytic and Esterifying Activities from Pseudomonas fragi CRDA 037 Grown on Whey

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