Biogenesis and Function of
            <i>Porphyromonas Gingivalis</i>
            Outer Membrane Vesicles

Xie, Hua

doi:10.2217/fmb.15.63

Cited by 58 publications

(62 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[14][15][16][17] Porphyromonas gingivalis possesses a vast array of effector molecules and systems that enables its colonization and survival in the oral cavity, communication with other bacteria and, ultimately, virulence. [18][19][20][21] Two distinct fimbriae composed of FimA and Mfa1, respectively, are the most well-documented virulence factors of P gingivalis. 22 Fimbriae contribute to colonization, biofilm formation, cell invasion, bone resorption, and the evasion of the host defense systems.…”

Section: Introductionmentioning

confidence: 99%

Characterization and development of SAPP as a specific peptidic inhibitor that targets Porphyromonas gingivalis

Lamont

Chazin

et al. 2018

Molecular Oral Microbiology

View full text Add to dashboard Cite

SUMMARY Porphyromonas gingivalis is a keystone bacterium in the oral microbial communities that elicits a dysbiosis between the microbiota and the host. Therefore, inhibition of this organism in dental plaques has been one of strategies for preventing and treating chronic periodontitis. We previously identified a Streptococcal ArcA derived Anti-P. gingivalils Peptide (SAPP) that in vitro, is capable of repressing the expression of several virulence genes in the organism. This leads to a significant reduction in P. gingivalis virulence potential, including its ability to colonize on the surface of Streptococcus gordonii, to invade human oral epithelial cells, and to produce gingipains. In this study, we showed that SAPP had minimal cytotoxicity to human oral keratinocytes and gingival fibroblasts. We observed that SAPP directly bound to the cell surface of P. gingivalis, and that alterations in the sequence at the N-terminus of SAPP diminished its abilities to interact with P. gingivalis cells and repressed the expression of virulence genes. Most strikingly, we demonstrated using an ex-vivo assay that besides its inhibitory activity against P. gingivalis colonization, SAPP could also reduce the levels of several other oral Gram-negative bacteria strongly associated with periodontitis in multispecies biofilms. Our results provide a platform for the development of SAPP-targeted therapeutics against chronic periodontitis.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization and development of SAPP as a specific peptidic inhibitor that targets Porphyromonas gingivalis

Lamont

Chazin

et al. 2018

Molecular Oral Microbiology

View full text Add to dashboard Cite

show abstract

“…P. gingivalis vesicles originate from outer membrane blebbing and contain mostly outer membrane components including lipopolysaccharides and outer membrane proteins (Xie, 2015) and exhibit the primary features of this organism. In fact, we recently demonstrated that P. gingivalis vesicles exhibit much higher invasive efficiency than their originating bacterial cells, although it appears that both invasive processes involve a clathrin-mediated endocytic machinery (Ho et al, 2015, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles

Guo

Chunga

et al. 2016

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Atherosclerosis, a chronic inflammatory disease of the blood vessels, is one of the most common causes of morbidity and mortality world-wide. Involvement of Porphyromonas gingivalis in atherosclerosis is supported by observations from epidemiological, clinical, immunological, and molecular studies. Previously we reported that P. gingivalis vesicles have a much higher invasive efficiency than their originating cells. Here, we further compare the role of P. gingivalis cells and their vesicles in expression of chemoattractant proteins including CXCL1, CXCL2, and CXCL8, and adhesive molecules such as E-selectin in human umbilical vein endothelial cells (HUVECs). Both P. gingivalis 33277 cells and vesicles were able to up-regulate expression of these molecules, while the vesicles acted as more potent inducers of the inflammatory response associated with the development of atherosclerosis, consequently resulting in significant monocyte adhesion to a monolayer of HUVECs. Interestingly, we found that elevated expression of CXCL8 and E-selectin in endothelial cells induced by P. gingivalis correlated with the invasive ability of P. gingivalis cells and vesicles. Non-invasive bacterial cells and vesicles had no effect on expression of these genes. This study highlights the potential risk of P. gingivalis cells and vesicles in initiation of atherosclerosis and provides a potential target for the development of novel therapeutics against bacteria-associated atherosclerosis.

show abstract

“…Gram-negative organisms, including known oral pathobionts, have been shown to play important roles in interspecies interaction and to contain factors that trigger an inflammatory response (37,38).…”

mentioning

confidence: 99%

Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont,Desulfobulbus oralis

Cross

Chirania

Xiong

et al. 2018

mBio

View full text Add to dashboard Cite

The human oral microbiota encompasses representatives of many bacterial lineages that have not yet been cultured. Here we describe the isolation and characterization of previously uncultured Desulfobulbus oralis, the first humanassociated representative of its genus. As mammalian-associated microbes rarely have free-living close relatives, D. oralis provides opportunities to study how bacteria adapt and evolve within a host. This sulfate-reducing deltaproteobacterium has adapted to the human oral subgingival niche by curtailing its physiological repertoire, losing some biosynthetic abilities and metabolic independence, and by dramatically reducing environmental sensing and signaling capabilities. The genes that enable free-living Desulfobulbus to synthesize the potent neurotoxin methylmercury were also lost by D. oralis, a notably positive outcome of host association. However, horizontal gene acquisitions from other members of the microbiota provided novel mechanisms of interaction with the human host, including toxins like leukotoxin and hemolysins. Proteomic and transcriptomic analysis revealed that most of those factors are actively expressed, including in the subgingival environment, and some are secreted. Similar to other known oral pathobionts, D. oralis can trigger a proinflammatory response in oral epithelial cells, suggesting a direct role in the development of periodontal disease.IMPORTANCE Animal-associated microbiota likely assembled as a result of numerous independent colonization events by free-living microbes followed by coevolution with their host and other microbes. Through specific adaptation to various body sites and physiological niches, microbes have a wide range of contributions, from beneficial to disease causing. Desulfobulbus oralis provides insights into genomic and physiological transformations associated with transition from an open environment to a host-dependent lifestyle and the emergence of pathogenicity. Through a multifaceted mechanism triggering a proinflammatory response, D. oralis is a novel periodontal pathobiont. Even though culture-independent approaches can provide insights into the potential role of the human microbiome "dark matter," cultivation and experimental characterization remain important to studying the roles of individual organisms in health and disease.KEYWORDS evolution, genome analysis, oral microbiology, periodontitis, proteomics

show abstract

Biogenesis and Function of Porphyromonas Gingivalis Outer Membrane Vesicles

Cited by 58 publications

References 80 publications

Characterization and development of SAPP as a specific peptidic inhibitor that targets Porphyromonas gingivalis

Characterization and development of SAPP as a specific peptidic inhibitor that targets Porphyromonas gingivalis

Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles

Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont,Desulfobulbus oralis

Contact Info

Product

Resources

About