Biogenesis and release of endothelial extracellular vesicles: Morphological aspects

Elsner, Clara; Ergün, Süleyman; Wagner, Nicole

doi:10.1016/j.aanat.2022.152006

Cited by 9 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microvascular endothelium is activated in various disease states (e.g. severe sepsis) by the cytokine TNF-α, triggering the release of endothelial EVs into the bloodstream [ 29 ]. Plasma concentrations of TNF-α were shown to be increased in patients with severe malaria and TNF-α levels are discussed to be a potential prognostic biomarker [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Surface proteome of plasma extracellular vesicles as mechanistic and clinical biomarkers for malaria

et al. 2023

View full text Add to dashboard Cite

Purpose Malaria is a life-threatening mosquito-borne disease caused by Plasmodium parasites, mainly in tropical and subtropical countries. Plasmodium falciparum (P. falciparum) is the most prevalent cause on the African continent and responsible for most malaria-related deaths globally. Important medical needs are biomarkers for disease severity or disease outcome. A potential source of easily accessible biomarkers are blood-borne small extracellular vesicles (sEVs). Methods We performed an EV Array to find proteins on plasma sEVs that are differentially expressed in malaria patients. Plasma samples from 21 healthy subjects and 15 malaria patients were analyzed. The EV array contained 40 antibodies to capture sEVs, which were then visualized with a cocktail of biotin-conjugated CD9, CD63, and CD81 antibodies. Results We detected significant differences in the protein decoration of sEVs between healthy subjects and malaria patients. We found CD106 to be the best discrimination marker based on receiver operating characteristic (ROC) analysis with an area under the curve of > 0.974. Additional ensemble feature selection revealed CD106, Osteopontin, CD81, major histocompatibility complex class II DR (HLA-DR), and heparin binding EGF like growth factor (HBEGF) together with thrombocytes to be a feature panel for discrimination between healthy and malaria. TNF-R-II correlated with HLA-A/B/C as well as CD9 with CD81, whereas Osteopontin negatively correlated with CD81 and CD9. Pathway analysis linked the herein identified proteins to IFN-γ signaling. Conclusion sEV-associated proteins can discriminate between healthy individuals and malaria patients and are candidates for future predictive biomarkers. Trial registration The trial was registered in the Deutsches Register Klinischer Studien (DRKS-ID: DRKS00012518).

show abstract

Section: Discussionmentioning

confidence: 99%

Surface proteome of plasma extracellular vesicles as mechanistic and clinical biomarkers for malaria

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Annexin A1 which belongs to the family of Ca 2+ -dependent phospholipid-binding membrane proteins has been identified as a specific marker of MVs ( Jeppesen et al, 2019 ). In mammals, MVs can be released by almost all cell types such as blood cells (platelets, leukocytes, and erythrocytes) ( Wolf, 1967 ), endothelial cells ( Elsner et al, 2023 ), and vascular smooth muscle cells ( Boulanger et al, 2006 ). Apart from differences in their size and biogenesis, MVs and exosomes express different surface molecules used as biomarkers for their identification ( Wu et al, 2013 ).…”

Section: Extracellular Vesicles (Evs) Their Origin Subtypes and Compo...mentioning

confidence: 99%

Wharton’s jelly mesenchymal stem cells: a concise review of their secretome and prospective clinical applications

Drobiova,

Sindhu,

Ahmad

et al. 2023

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Accumulating evidence indicates that most primary Wharton’s jelly mesenchymal stem cells (WJ-MSCs) therapeutic potential is due to their paracrine activity, i.e., their ability to modulate their microenvironment by releasing bioactive molecules and factors collectively known as secretome. These bioactive molecules and factors can either be released directly into the surrounding microenvironment or can be embedded within the membrane-bound extracellular bioactive nano-sized (usually 30–150 nm) messenger particles or vesicles of endosomal origin with specific route of biogenesis, known as exosomes or carried by relatively larger particles (100 nm–1 μm) formed by outward blebbing of plasma membrane called microvesicles (MVs); exosomes and MVs are collectively known as extracellular vesicles (EVs). The bioactive molecules and factors found in secretome are of various types, including cytokines, chemokines, cytoskeletal proteins, integrins, growth factors, angiogenic mediators, hormones, metabolites, and regulatory nucleic acid molecules. As expected, the secretome performs different biological functions, such as immunomodulation, tissue replenishment, cellular homeostasis, besides possessing anti-inflammatory and anti-fibrotic effects. This review highlights the current advances in research on the WJ-MSCs’ secretome and its prospective clinical applications.

show abstract

Insight into endothelial cell-derived extracellular vesicles in cardiovascular disease: Molecular mechanisms and clinical implications

Wang,

2024

Pharmacological Research

View full text Add to dashboard Cite

Biogenesis and release of endothelial extracellular vesicles: Morphological aspects

Cited by 9 publications

References 43 publications

Surface proteome of plasma extracellular vesicles as mechanistic and clinical biomarkers for malaria

Surface proteome of plasma extracellular vesicles as mechanistic and clinical biomarkers for malaria

Wharton’s jelly mesenchymal stem cells: a concise review of their secretome and prospective clinical applications

Insight into endothelial cell-derived extracellular vesicles in cardiovascular disease: Molecular mechanisms and clinical implications

Contact Info

Product

Resources

About