Biogenesis of cytoplasmic lipid droplets: From the lipid ester globule in the membrane to the visible structure

Ohsaki, Yuki; Cheng, Jinglei; Suzuki, Michitaka; Shinohara, Yuki; Fujita, Akikazu; Fujimoto, Toyoshi

doi:10.1016/j.bbalip.2008.10.002

Cited by 136 publications

(139 citation statements)

References 102 publications

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“…Turró et al reported a proteome study on hepatocytes showing calnexin was present as one of the associated proteins in the LD fraction ( 40 ). Recently, Fujimoto et al proposed that LDs and ERs form complexes, which explains the unique structure called the "apoB crescent" ( 38,39 ). Since our iLD fractions showed HDL-like density and much larger particle sizes, it is possible that there are LD-ER complexes like the apoB crescent in the iLD fractions.…”

Section: Induction Of Adrp-associated Ild Fractions In Huh-7 Human Hementioning

confidence: 99%

Glucagon regulates intracellular distribution of adipose differentiation-related protein during triacylglycerol accumulation in the liver

Takahashi

Sasabe

Ohshima

et al. 2010

Journal of Lipid Research

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Supplementary key words fatty liver • hepatocytes • HuH-7 • lipid droplets • NASH • phospholipase C • protein kinase A • sucrose density gradient centrifugationIntracellular lipid droplets (LD) are cellular organelles observed ubiquitously in physiological and pathological conditions. LDs are composed of a core of neutral lipids containing cholesteryl esters and/or triacylglycerol (TG), a surrounding surface monolayer of phospholipids, and several associated proteins. LDs are considered to be a storage site of neutral lipids and to play a role in wholebody energy homeostasis ( 1-3 ).A number of proteomic studies on LD in various cells have revealed the unique protein profi les of LD ( 4-8 ). A Abstract Cellular lipid droplets (LD) are organelles involved in cellular lipid metabolism. When liver cellular components were fractionated using sucrose density gradient centrifugation, adipose differentiation-related protein (ADRP) was distributed in both the top and bottom fractions, which correspond to the LD and membranous fractions, respectively, in the mouse liver under normal feeding conditions. After overnight fasting, triacylglycerol and ADRP increased nearly 2.5-fold in the mouse liver, and a portion appeared in the intermediate-density LD (iLD) fractions. ADRP in the iLD fractions was also increased in a mouse nonalcoholic steatohepatitis model induced by methione/choline-defi cient diet. When HuH-7 human hepatoma cells were incubated with oleic acid for 24 h, the amount of ADRP increased, and it was distributed in both the LD and membrane fractions. However, ADRP appeared in the iLD fractions upon treatment of HuH-7 cells with glucagon. This behavior of ADRP was cAMP-dependent, as the ADRP-positive iLD fractions were induced by dibutylyl cAMP and were blocked by protein kinase A inhibitors. A portion of ADRP colocalized microscopically with calnexin, which is present in the iLD fractions, by treatment of HuH-7 cells or human primary hepatocytes with oleic acid and glucagon, but not by treatment with oleic acid alone. Glucagon has a role in the reorganization of endoplasmic reticulum membranes to generate ADRP-associated lipid-poor particles in hepatic cells, which is related to LD formation during lipid storage. -Takahashi, K., N. Sasabe, K. Ohshima, K. Kitazato, R. Kato, Y. Masuda, M. Tsurumaki, T. Obama, S-i. Okudaira, J. Aoki, H. Arai, T. Yamaguchi, and H. Itabe. MEXT, 2005MEXT, -2009 Abbreviations: Ab, antibody; ADRP, adipose differentiation-related protein; ER, endoplasmic reticulum; GRP78/BiP, glucose-regulated protein of 78-kDa/binding protein; iLD, intermediate-density lipid droplet; LD, lipid droplet; MCD, methione/choline defi cient; MTP, microsomal triacylglycerol transfer protein; NASH; nonalcoholic steatohepatitis; OA, oleic acid; PAT, perilipin-ADRP-TIP47; PLP, 4% paraformaldehydelysine-sodium periodate; PC, phosphatidylcholine; PKA, protein kinase A; PKI, PKI14-22amide; PLC, phospholipase C; PNS, post-nuclear supernatant; PPAR, peroxisome proliferator-activated receptor; Rp, Rp-8-Br-cAMPS; SCD...

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Section: Induction Of Adrp-associated Ild Fractions In Huh-7 Human Hementioning

confidence: 99%

Glucagon regulates intracellular distribution of adipose differentiation-related protein during triacylglycerol accumulation in the liver

Takahashi

Sasabe

Ohshima

et al. 2010

Journal of Lipid Research

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“…Some scientists believe that they are formed in cytoplasm as naked droplets (Lung & Weselake, 2006) which are later surrounded by a half unit membrane, others suggest that both ER membranes take part in their biogenesis (Robenek et al, 2011). However, the most common hypothesis is based on old ultrastructural observations (Frey-Wyssling et al, 1963) of plant cells which indicate that ER contributes to lipid bodies formation resulting from the accumulation of lipids between leaflets of a phospholipid bilayer (Fujimoto et al, 2008;Ducharme & Bickel, 2008;Guo et al, 2009;Ohsaki et al, 2009). …”

Section: Lipid Bodies -Biogenesis and Lipid Synthesismentioning

confidence: 99%

Lipotubuloids - Structure and Function

Kwiatkowska¹,

Popłońska²,

Stępiński³

et al. 2012

Advances in Selected Plant Physiology Aspects

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The book provides general principles and new insights of some plant physiology aspects covering abiotic stress, plant water relations, mineral nutrition and reproduction. Plant response to reduced water availability and other abiotic stress (e.g. metals) have been analysed through changes in water absorption and transport mechanisms, as well as by molecular and genetic approach. A relatively new aspects of fruit nutrition are presented in order to provide the basis for the improvement of some fruit quality traits. The involvement of hormones, nutritional and proteomic plant profiles together with some structure/function of sexual components have also been addressed. Written by leading scientists from around the world it may serve as source of methods, theories, ideas and tools for students, researchers and experts in that areas of plant physiology.

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“…The material was not incubated (embryo cells) or incubated (root cells) in 15 μM taxol dissolved in the microtubule stabilizing buffer MSB (100 mM PIPES, 1 mM MgCl 2 , 5 mM EGTA) pH 7.2 at RT for 2 h then fixed in a mixture of 1% OsO 4 and 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.2) for 2 h and postfixed in 1% OsO 4 in the same buffer at 4°C for 3 h.…”

Section: Methodsmentioning

confidence: 99%

“…Lipid bodies (lipid droplets, oleosomes) are the focus of interest for many scientists, as demonstrated by the large number of reviews concerning them [1][2][3][4][5][6][7]. They have become an important, and fascinating, object of research because they take part in many intracellular processes.…”

Section: Introductionmentioning

confidence: 99%

DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules

Kwiatkowska¹,

Stępiński²,

Popłońska³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations. Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated in lipid synthesis. A similar phenomenon may take place in

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Biogenesis of cytoplasmic lipid droplets: From the lipid ester globule in the membrane to the visible structure

Cited by 136 publications

References 102 publications

Glucagon regulates intracellular distribution of adipose differentiation-related protein during triacylglycerol accumulation in the liver

Glucagon regulates intracellular distribution of adipose differentiation-related protein during triacylglycerol accumulation in the liver

Lipotubuloids - Structure and Function

DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules

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