Biogenesis of MiRNA‐195 and its role in biogenesis, the cell cycle, and apoptosis

Junfeng, HE; Luo, Yang; Wan, Xinhong; De-qian, Jiang

doi:10.1002/jbt.20396

Cited by 114 publications

(72 citation statements)

References 27 publications

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“…This highlights that the study of a single disease, such as laryngeal cancer, is advantageous over a combination study of various types of head and neck tumors in multiple sites. miR-195 is an important member of the miRNA-15/16/195/424/497 family that is downregulated in various types of cancer, including liver, gastric, breast, bladder cancer and chronic lymphocytic leukemia, which suggests that miR-195 may be an important tumor suppressor (30). The present study represents the first report to demonstrate that miR-195 has reduced expression in laryngeal cancer; however, further investigation is required to confirm this result at the cellular level.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA differential expression spectrum and microRNA-125a-5p inhibition of laryngeal cancer cell proliferation

Yao

Wang

2017

Experimental and Therapeutic Medicine

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Abstract. The present study aimed to screen and analyze the differential expression spectrum of microRNA (miRNA) between laryngeal cancer tissue and surrounding normal laryngeal mucosa in order to provide an indication for further study to determine the role of miRNA in the initiation and development of laryngeal cancer. A total of 42 pairs of specimens of laryngeal carcinoma tissues and adjacent normal laryngeal mucosa were collected. A total of 10 pairs of specimens were randomly selected for miRNA microarray gene chip analysis, and the remaining 32 pairs of specimens were used for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) verification to identify miRNA that were differentially expressed in laryngeal cancer tissues. Methylthiazolyldiphenyl-tetrazolium bromide and clone formation assays were utilized to elucidate the physiological relevance of the miRNA miR-125a-5p on the proliferation of laryngeal cancer human epithelial type 2 (Hep2) cells. Results demonstrated that the expression levels of six miRNA were significantly downregulated in laryngeal carcinoma tissue, as identified by gene chip analysis and RT-qPCR (P<0.05). The six miRNA included let-7f-5p, miR-10a-5p, miR-125a-5p, miR-144-3p, miR-195-5p and miR-203. Compared with the control group, the proliferative ability of laryngeal cancer Hep2 cells was inhibited in a transfected miR-125a-mimics group. In contrast, proliferation was promoted in a transfected miR-125a-inhibitor group. In conclusion, the results of gene chip analysis were consistent with that of RT-qPCR. Results demonstrated that miRNA in laryngeal cancer and normal laryngeal mucosa exhibited evident differential expression, which may contribute to the laryngeal cancer incidence and invasion. miR-125a was able to inhibit the proliferation of Hep2 laryngeal cancer cells and, therefore, may serve as a novel target for laryngeal cancer biological therapy.

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Section: Discussionmentioning

confidence: 99%

MicroRNA differential expression spectrum and microRNA-125a-5p inhibition of laryngeal cancer cell proliferation

Yao

Wang

2017

Experimental and Therapeutic Medicine

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“…miR-124 is a novel member of these miRNAs, which has been characterized as a tumor suppressor in human cancers by targeting critical cancer-related pathways. It is shown that miR-124 could affect the STAT3 signal pathway and enhance the anticarcinogenic effect [9][10][11]. Notably, a recent study indicated that miR-124 is associated with tumor metastatic capability; however, the role of miR-124 in GBM proliferation and invasion remains unknown.…”

Section: Introductionmentioning

confidence: 95%

RETRACTED ARTICLE: miR-124 Acts as a Tumor Suppressor in Glioblastoma via the Inhibition of Signal Transducer and Activator of Transcription 3

Huang

et al. 2016

Mol Neurobiol

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MicroRNAs are important regulators of multiple cellular processes, and aberrant miRNA expression has been observed in human glioblastoma (GBM). The present study was to evaluate the level of miR-124 and signal transducer and activator of transcription 3 (STAT3) in GBM tissues and cells. We further investigated the molecular mechanisms of miR-124 and STAT3 in GBM cell lines U87 and U251. Here, we found that miR-124 expression was downregulated in GBM tissues and U87 and U251 cells (all p < 0.001) but not associated with blood routine (RBC, WBC count, etc.) and liver and renal function indicators (all p > 0.05). By contrast, STAT3 was upregulated. Furthermore, the expression of miR-124 was inversely proportional to that of STAT3 mRNA or protein (p = 0.013, p = 0.015, respectively). In vitro studies demonstrated that the overexpression of miR-124 played a suppressor role in the proliferation of U87 and U251 cells and promoted cell apoptosis. Luciferase reporter assays confirmed that miR-124 binding to the 3'-UTR regions of STAT3 inhibited the expression of STAT3 in U87 and U251 cells. However, the inhibitor of miR-124 promoted the expression of STAT3 and cell proliferation. In conclusion, our data suggest that miR-124 may have a potential role in treatment of GBM patients and that miR-124 is a novel regulator of invasiveness and tumorigenicity in GBM cells by targeting STAT3. The miR-124/STAT3 pathway may be a useful therapeutic agent in GBM patients.

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“…Apoptosis, also called programmed cell death, is critical in normal development, and in the initiation and progression of cancers (39). Mutated and deleted pro-apoptotic genes give rise to carcinogenesis and tumor growth.…”

Section: Gas5 Tumor-suppressive Functionsmentioning

confidence: 99%

Long non-coding RNA growth arrest-specific transcript 5 in tumor biology

2015

Oncology Letters

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Abstract. The recognition of the biological relevance of long non-coding RNA (lncRNA) molecules has only recently been recognized as one of the most significant advances in contemporary molecular biology. A growing body of evidence indicates that lncRNAs act not only as the intermediary between DNA and protein but also as significant protagonists of cellular functions. The dysregulation of lncRNAs has increasingly been linked to numerous human diseases, particularly cancers. Recent studies have demonstrated that the lncRNA growth arrest-specific transcript 5 (GAS5) was pervasively downexpressed in most human cancers compared with non-cancerous adjacent tissues including gastric, breast, lung and prostate cancer. In addition, patients with decreased GAS5 expression have a significantly poorer prognosis than those with higher expression. Furthermore, GAS5 is involved in the control of cell apoptosis, proliferation, metastasis, angiogenesis, DNA repair and tumor cell metabolism. This review provides an overview of the current knowledge concerning the role of GAS5 in tumor expression and biology function.

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Biogenesis of MiRNA‐195 and its role in biogenesis, the cell cycle, and apoptosis

Cited by 114 publications

References 27 publications

MicroRNA differential expression spectrum and microRNA-125a-5p inhibition of laryngeal cancer cell proliferation

MicroRNA differential expression spectrum and microRNA-125a-5p inhibition of laryngeal cancer cell proliferation

RETRACTED ARTICLE: miR-124 Acts as a Tumor Suppressor in Glioblastoma via the Inhibition of Signal Transducer and Activator of Transcription 3

Long non-coding RNA growth arrest-specific transcript 5 in tumor biology

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