Biogenesis of Ribosomes in Eukaryotes

Hadjiolov, A.A.

doi:10.1007/978-1-4615-7948-9_1

Cited by 23 publications

(25 citation statements)

References 407 publications

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“…Processing of pre-rRNA and assembly of ribosomes are intricately related (Hadjiolov, 1980;Lastick, 19801, and any condition that limits the supply of ribosomal proteins could have an effect on rRNA precursor maturation. Protein synthesis is inhibited by 50-70% at 42°C in HeLa cells (Hickey and Weber, 1982;McCormick and Penman, 1969).…”

Section: Discussionmentioning

confidence: 99%

Effect of heat shock on RNA metabolism in HeLa cells

Sadis

Hickey

Weber

1988

Journal Cellular Physiology

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Incubation of HeLa cells at 42 degrees C results in pronounced inhibition of the accumulation of 18S and 28S ribosomal RNA (rRNA) and non-heat shock polyadenylated messenger RNA (mRNA) in the cytoplasm. Accumulation of transfer RNA and 5S ribosomal RNA is not affected. Transcription of rRNA precursor is reduced to approximately 50% of the 37 degrees C rate after 10 min of hyperthermia and declines to 30% of the control rate after 1 hr. In contrast, the accumulation of mature rRNA in the cytoplasm is inhibited more than 95%. Quantitative hybridization experiments and Northern blot analysis detect little accumulation of rRNA precursor sequences in nuclei, suggesting that the majority of the rRNA that is synthesized is degraded. Heat stress at 42 degrees C was found to have little effect on transcription of most non-heat shock mRNAs. However, accumulation of individual non-heat shock mRNAs in the cytoplasm proceeds at reduced rates. These results indicate that the primary effect of elevated temperature on RNA metabolism in mammalian cells is inhibition of processing and/or transport. Despite this, steady-state levels of abundant and rapidly turning over mRNA species remain unchanged during prolonged heat stress. We find that the half-life of c-myc mRNA increases greater than twofold at 42 degrees C. Thus, 42 degrees C heat stress appears to inhibit both accumulation and turnover of non-heat shock mRNA.

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Section: Discussionmentioning

confidence: 99%

Effect of heat shock on RNA metabolism in HeLa cells

Sadis

Hickey

Weber

1988

Journal Cellular Physiology

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“…Ribosomal subunits are made in discrete stages within the granular component of the nucleolus. Initially, the 45s rRNA precursor associates with both 5s rRNA and r-proteins forming a complex referred to as an 80s preribosome (reviewed by Hadjiolov 1980). Maturation of the preribosome parallels the processing of the primary rRNA transcript, suggesting a close link between these two events.…”

Section: Ribosome Assembly and Turnovermentioning

confidence: 98%

“…The primary 45s rRNA transcript is processed in sequential steps into 18S, 28S, and 5.8s rRNAs prior to their incorporation into large and small ribosomal subunits (reviewed by Hadjiolov 1980). snRNPs are involved in splicing and processing reactions required for mRNA maturation.…”

Section: Regulation Of Pre-rrna Processingmentioning

confidence: 99%

Control points in eucaryotic ribosome biogenesis

Larson¹,

Zahradka²,

Sells³

1991

Biochem. Cell Biol.

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Ribosome biogenesis in eucaryotic cells involves the coordinated synthesis of four rRNA species, transcribed by RNA polymerase I (18S, 28S, 5.8S) and RNA polymerase III (5S), and approximately 80 ribosomal proteins translated from mRNAs synthesized by RNA polymerase II. Assembly of the ribosomal subunits in the nucleolus, the site of 45S rRNA precursor gene transcription, requires the movement of 5S rRNA and ribosomal proteins from the nucleoplasm and cytoplasm, respectively, to this structure. To integrate these events and ensure the balanced production of individual ribosomal components, different strategies have been developed by eucaryotic organisms in response to a variety of physiological changes. This review presents an overview of the mechanisms modulating the production of ribosomal precursor molecules and the rate of ribosome biogenesis in various biological systems.

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“…We believe, however, that it is unlikely that the specific inhibition of production of cytoplasmic 28S rRNA is mediated at the level of assembly. The processing of pre-rRNA and its assembly into ribonucleoprotein particles are coupled processes, in which addition of proteins to RNA begins very soon after synthesis of 45S pre-rRNA and continues in parallel with rRNA processing in the nucleolus (10,14,24). Thus inhibition of any step in the assembly process would be predicted to be reflected in a corresponding alteration in nucleolar rRNA processing, a prediction that is not borne out during the earliest phases of AdS infection ( Fig.…”

Section: Downloaded Frommentioning

confidence: 99%

Effects of adenovirus infection on rRNA synthesis and maturation in HeLa cells.

Castiglia¹,

Flint²

1983

Mol. Cell. Biol.

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Virology 40:893-902, 1970). However, the effects on 28S cytoplasmic rRNA were observed considerably earlier in the infectious cycle than those on 18S rRNA. The inhibition of cellular protein synthesis and of the appearance in the cytoplasm of labeled cellular mRNA sequences (G. A. Beltz and S. J. Flint, J. Mol. Biol. 131:353-373, 1979) were also monitored in infected cultures. During the later periods of an infectious cycle, from 18 h after infection, nucleolar rRNA synthesis and processing and exit of 18S rRNA from the nucleus were inhibited, probably reflecting the failure of infected cells to synthesize normal quantities of ribosomal proteins. The earliest responses of cellular RNA metabolism to adenovirus infection were, however, the rapid and apparently coordinate reductions in the levels of newly synthesized 28S rRNA and cellular mRNA sequences entering the cytoplasm.

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Biogenesis of Ribosomes in Eukaryotes

Cited by 23 publications

References 407 publications

Effect of heat shock on RNA metabolism in HeLa cells

Effect of heat shock on RNA metabolism in HeLa cells

Control points in eucaryotic ribosome biogenesis

Effects of adenovirus infection on rRNA synthesis and maturation in HeLa cells.

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