Biogenesis of silver nanoparticles using endophytic fungus &lt;em&gt;Pestalotiopsis microspora &lt;/em&gt;and evaluation of their antioxidant and anticancer activities

Netala, Vasudeva Reddy; Bethu, Murali Satyanarayana; Bobbu, Pushpalatha; Baki, Vijaya Bhaskar; S, Aishwarya; Rao, J. Venkateswara; Vijaya, Tartte

doi:10.2147/ijn.s112857

Cited by 157 publications

(60 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…21,30,46 Collectively, the preliminary data that TUB-A and PdNPs alter cellular morphology provide evidence for their cytotoxicity. The well-established morphological criteria, such as distinct cell shrinkage and membrane blebbing, strongly correlate with apoptotic changes, such as chromatin condensation, nuclear and cell fragmentation, and internucleosomal DNA cleavage.…”

mentioning

confidence: 89%

Combination of palladium nanoparticles and tubastatin-A potentiates apoptosis in human breast cancer cells: a novel therapeutic approach for cancer

Yuan¹,

Peng²,

Gurunathan³

2017

IJN

View full text Add to dashboard Cite

Background Breast cancer is the most common malignant disease that occurs in women. Histone deacetylase (HDAC) inhibition has recently emerged as an effective and attractive target for the treatment of cancer. The aim of this study was to investigate the efficacy of a combined treatment of tubastatin A (TUB-A) and palladium nanoparticles (PdNPs) against MDA-MB-231 human breast cancer cells using two different cytotoxic agents that work by two different mechanisms, thereby decreasing the probability of chemoresistance in cancer cells and increasing the efficacy of toxicity, to provide efficient therapy for advanced stage of cancer without any undesired side effects. Methods PdNPs were synthesized using a novel biomolecule called R-phycoerythrin and characterized using various analytical techniques. The combinatorial effect of TUB-A and PdNPs was assessed by various cellular and biochemical assays and also by gene expression analysis. Results The biologically synthesized PdNPs had an average size of 25 nm and were spherical in shape. Treatment of MDA-MB-231 human breast cancer cells with TUB-A or PdNPs showed a dose-dependent effect on cell viability. The combination of 4 μM TUB-A and 4 μM PdNPs had a significant inhibitory effect on cell viability compared with either TUB-A or PdNPs alone. The combinatorial treatment also had a more pronounced effect on the inhibition of HDAC activity and enhanced apoptosis by regulating various cellular and biochemical changes. Conclusion Our results suggest that there was a strong synergistic interaction between TUB-A and PdNPs in increasing apoptosis in human breast cancer cells. These data provide an important preclinical basis for future clinical trials on this drug combination. This combinatorial treatment increased therapeutic potentials, thereby demonstrating a relevant targeted therapy for breast cancer. Furthermore, we have provided the first evidence for the combinatorial effect and mechanism of toxicity of TUB-A and PdNPs in human breast cancer cells. The novelties of the study were identification of a combination therapy that consists of suitable therapeutic molecules that kill cancer cells and also exploration of two different possible mechanisms involved to reduce chemoresistance in cancer cells.

show abstract

mentioning

confidence: 89%

Combination of palladium nanoparticles and tubastatin-A potentiates apoptosis in human breast cancer cells: a novel therapeutic approach for cancer

Yuan¹,

Peng²,

Gurunathan³

2017

IJN

View full text Add to dashboard Cite

show abstract

“…The 1:25 solution had the highest negative value of the zeta potential, and so was the most stable because the repulsive forces between negatively charged particles prevent agglomeration. [32][33][34] DLS is used to determine the size of the nanoparticle considering the metallic nucleus of the NP and the cloud of electrons that surrounds it as well as defining the zeta potential. 35,36 The results of the DLS analysis demonstrated that the size of the biosynthesized 1:100 AgNPs was between 36 and 486 nm, of the 1:50 AgNPs between 30 and 409 nm, and of the 1:25 AgNPs between 12 and 409 nm (Figure 3).…”

Section: Dls and Zeta Potentialmentioning

confidence: 99%

Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus <em>Duddingtonia flagrans</em>

Silva

Oliveira

Keijok

et al. 2017

IJN

View full text Add to dashboard Cite

The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the extracellular synthesis of highly stable silver NPs (AgNPs) using the nematophagous fungus Duddingtonia flagrans (AC001). The fungal cell-free filtrate was analyzed by the Bradford method and 3,5-dinitrosalicylic acid assay and used to synthesize the AgNPs in the presence of a 1 mM AgNO 3 solution. They have been characterized by UV–Vis spectroscopy, X-ray diffraction, transmission electron microscopy, dynamic light scattering, Zeta potential measurements, Fourier-transform infrared, and Raman spectroscopes. UV–Vis spectroscopy confirmed bioreduction, while X-ray diffractometry established the crystalline nature of the AgNPs. Dynamic light scattering and transmission electron microscopy images showed approximately 11, 38 nm monodisperse and quasispherical AgNPs. Zeta potential analysis was able to show a considerable stability of AgNPs. The N–H stretches in Fourier-transform infrared spectroscopy indicate the presence of protein molecules. The Raman bands suggest that chitinase was involved in the growth and stabilization of AgNPs, through the coating of the particles. Our results show that the NPs we synthesized have good stability, high yield, and monodispersion.

show abstract

“…32 MCF-7 cells and MCF-7/ADR cells were seeded in 96-well plates at a density of 5×10 3 cells per well and incubated for 24 h, respectively. The growth medium was replaced with fresh medium containing an indicated concentration (0.5, 1, 2, 4, 6, 8, 10 and 12 µg/mL for MCF-7 cancer cells; 1, 5, 10, 50, 100, 150, 200 and 300 µg/mL for MCF-7/ADR cancer cells) of the tested formulations (AT-M/DOX, pH T-M/DOX, endo E-M/ DOX, PT-M/DOX and DOX solution).…”

Section: In Vitro Cell Cytotoxicitymentioning

confidence: 99%

Rational design of multifunctional micelles against doxorubicin-sensitive and doxorubicin-resistant MCF-7 human breast cancer cells

Hong

Shi

Qiao

et al. 2017

IJN

View full text Add to dashboard Cite

Even though a tremendous number of multifunctional nanocarriers have been developed to tackle heterogeneous cancer cells, little attention has been paid to elucidate how to rationally design a multifunctional nanocarrier. In this study, three individual functions (active targeting, stimuli-triggered release and endo-lysosomal escape) were evaluated in doxorubicin (DOX)-sensitive MCF-7 cells and DOX-resistant MCF-7/ADR cells by constructing four kinds of micelles with active-targeting (AT-M), passive targeting, pH-triggered release ( pH T-M) and endo-lysosomal escape ( endo E-M) function, respectively. AT-M demonstrated the strongest cytotoxicity against MCF-7 cells and the highest cellular uptake of DOX due to the folate-mediated endocytosis. However, AT-M failed to exhibit the best efficacy against MCF-7/ADR cells, while endo E-M exhibited the strongest cytotoxicity against MCF-7/ADR cells and the highest cellular uptake of DOX due to the lowest elimination of DOX from the cells. This was attributed to the carrier-facilitated endo-lysosomal escape of DOX, which avoided exocytosis by lysosome secretion, resulting in an effective accumulation of DOX in the cytoplasm. The enhanced elimination of DOX from the MCF-7/ADR cells also accounted for the remarkable decrease in cytotoxicity against the cells of AT-M. Three micelles were further evaluated with MCF-7 cells and MCF-7/ADR-resistant cells xenografted mice model. In accordance with the in vitro results, AT-M and endo E-M demonstrated the strongest inhibition on the MCF-7 and MCF-7/ADR xenografted tumor, respectively. Active targeting and active targeting in combination with endo-lysosomal escape have been demonstrated to be the primary function for a nanocarrier against doxorubicin-sensitive and doxorubicin-resistant MCF-7 cells, respectively. These results indicate that the rational design of multifunctional nanocarriers for cancer therapy needs to consider the heterogeneous cancer cells and the primary function needs to be integrated to achieve effective payload delivery.

show abstract

Biogenesis of silver nanoparticles using endophytic fungus <em>Pestalotiopsis microspora </em>and evaluation of their antioxidant and anticancer activities

Cited by 157 publications

References 50 publications

Combination of palladium nanoparticles and tubastatin-A potentiates apoptosis in human breast cancer cells: a novel therapeutic approach for cancer

Combination of palladium nanoparticles and tubastatin-A potentiates apoptosis in human breast cancer cells: a novel therapeutic approach for cancer

Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus <em>Duddingtonia flagrans</em>

Rational design of multifunctional micelles against doxorubicin-sensitive and doxorubicin-resistant MCF-7 human breast cancer cells

Contact Info

Product

Resources

About