Biogenic action of Lactobacillus plantarum SBT2227 promotes sleep in Drosophila melanogaster

Ko, Taro; Murakami, Hiroki; Kamikouchi, Azusa; Ishimoto, Hiroshi

doi:10.1016/j.isci.2022.104626

Cited by 3 publications

(8 citation statements)

References 98 publications

(120 reference statements)

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“…Furthermore, BA2786 significantly shortened the sleep latency but did not alter the length and number of sleep bouts during the night or the waking activity index during the daytime. These characteristics are similar to the sleep responses of flies after the administration of LP2227 (Ko et al, 2022). Fly sleep can be categorized into several phenomena, such as morning or evening sleep, the onset of night‐time sleep, the second half of night‐time sleep, and falling asleep, each of which are regulated by distinct mechanisms (Agosto et al, 2008; Gmeiner et al, 2013; Liang et al, 2019).…”

Section: Discussionsupporting

confidence: 75%

“…One of the upregulated genes in BA2786‐fed flies was insulin‐like receptor ( InR ), which was also upregulated in flies fed LP2227, a L . plantarum strain that promotes sleep in fruit flies (Ko et al, 2022) (Table S1). Therefore, we focused on InR and examined the effects of BA2786 on sleep in an InR hypomorphic mutant.…”

Section: Resultsmentioning

confidence: 99%

“…Fly food consumption of BA2786 or BA003 and control food was measured as previously reported, with some modifications (Ko et al, 2022; Shell et al, 2018; Wu et al, 2020). Briefly, blue dye (Blue No.…”

Section: Methodsmentioning

confidence: 99%

“…We recently identified Lactiplantibacillus plantarum SBT2227 (hereafter, referred to as LP2227), which promotes sleep in D. melanogaster (Ko et al, 2022). L. plantarum is a gram‐positive lactic acid bacterium present in several niches, such as the gastrointestinal tract, feces, meat, vegetables, and dairy substrates (Siezen et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Behavioral screening of sleep‐promoting effects of human intestinal and food‐associated bacteria on Drosophila melanogaster

Murakami

Kobayashi

et al. 2023

Genes to Cells

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Commensal microbes influence various aspects of vertebrate and invertebrate brain function. We previously reported that Lactiplantibacillus plantarum SBT2227 promotes sleep in the fruit fly, Drosophila melanogaster. However, how widely the sleep‐promoting effects are conserved in gut bacterial species remains unknown. In this study, we orally administered human intestinal and food‐associated bacterial species (39 in total) to flies and investigated their effects on sleep. Six species of bacteria were found to have significant sleep‐promoting effects. Of these, we further investigated Bifidobacterium adolescentis, which had the greatest sleep‐promoting effect, and found that the strength of the sleep effect varied among strains of the same bacterial species. The B. adolescentis strains BA2786 and BA003 showed strong and weak effects on sleep, respectively. Transcriptome characteristics compared between the heads of flies treated with BA2786 or BA003 revealed that the gene expression of the insulin‐like receptor (InR) was increased in BA2786‐fed flies. Furthermore, a heterozygous mutation in InR suppressed the sleep‐promoting effect of BA2786. These results suggest that orally administered sleep‐promoting bacteria (at least BA2786), may act on insulin signaling to modulate brain function for sleep.

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Section: Discussionsupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Behavioral screening of sleep‐promoting effects of human intestinal and food‐associated bacteria on Drosophila melanogaster

Murakami

Kobayashi

et al. 2023

Genes to Cells

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“…Other studies showed that some bacterial strains could modulate the expression of gut peptides. For example, Ko et al (2022) reported that the administration of L. plantarum SBT2227 promotes sleep in Drosophila melanogaster through the induction of neuropeptide F (a homolog of mammalian neuropeptide Y; Ko et al, 2022 ). On the other hand, different types of proteases are produced by the gut microbiome, which results in the generation of a large number of peptides during the digestion of food proteins.…”

Section: Other Microbiota-produced Neuroactive Metabolitesmentioning

confidence: 99%

Neuromicrobiology, an emerging neurometabolic facet of the gut microbiome?

et al. 2023

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The concept of the gut microbiome is emerging as a metabolic interactome influenced by diet, xenobiotics, genetics, and other environmental factors that affect the host’s absorption of nutrients, metabolism, and immune system. Beyond nutrient digestion and production, the gut microbiome also functions as personalized polypharmacy, where bioactive metabolites that our microbes excrete or conjugate may reach systemic circulation and impact all organs, including the brain. Appreciable evidence shows that gut microbiota produce diverse neuroactive metabolites, particularly neurotransmitters (and their precursors), stimulating the local nervous system (i.e., enteric and vagus nerves) and affecting brain function and cognition. Several studies have demonstrated correlations between the gut microbiome and the central nervous system sparking an exciting new research field, neuromicrobiology. Microbiome-targeted interventions are seen as promising adjunctive treatments (pre-, pro-, post-, and synbiotics), but the mechanisms underlying host-microbiome interactions have yet to be established, thus preventing informed evidence-based therapeutic applications. In this paper, we review the current state of knowledge for each of the major classes of microbial neuroactive metabolites, emphasizing their biological effects on the microbiome, gut environment, and brain. Also, we discuss the biosynthesis, absorption, and transport of gut microbiota-derived neuroactive metabolites to the brain and their implication in mental disorders.

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Probiotic Lactobacillus spp. improves Drosophila memory by increasing lactate dehydrogenase levels in the brain mushroom body neurons

Ho,

Tsai,

Lai

et al. 2024

Gut Microbes

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Probiotics are live microorganisms that offer potential benefits to their hosts and can occasionally influence behavioral responses. However, the detailed mechanisms by which probiotics affect the behavior of their hosts and the underlying biogenic effects remain unclear. Lactic acid bacteria, specifically Lactobacillus spp. are known probiotics. Drosophila melanogaster , commonly known as the fruit fly, is a well-established model organism for investigating the interaction between the host and gut microbiota in translational research. Herein, we showed that 5-day administration of Lactobacillus acidophilus (termed GMNL-185) or Lacticaseibacillus rhamnosus (termed GMNL-680) enhances olfactory-associative memory in Drosophila . Moreover, a combined diet of GMNL-185 and GMNL-680 demonstrated synergistic effects on memory functions. Live brain imaging revealed a significant increase in calcium responses to the training odor in the mushroom body β and γ lobes of flies that underwent mixed feeding with GMNL-185 and GMNL-680. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and whole-mount brain immunohistochemistry revealed significant upregulation of lactate dehydrogenase (LDH) expression in the fly brain following the mixed feeding. Notably, the genetic knockdown of Ldh in neurons, specifically in mushroom body, ameliorated the beneficial effects of mixed feeding with GMNL-185 and GMNL-680 on memory improvement. Altogether, our results demonstrate that supplementation with L. acidophilus and L. rhamnosus enhances memory functions in flies by increasing brain LDH levels.

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Biogenic action of Lactobacillus plantarum SBT2227 promotes sleep in Drosophila melanogaster

Cited by 3 publications

References 98 publications

Behavioral screening of sleep‐promoting effects of human intestinal and food‐associated bacteria on Drosophila melanogaster

Behavioral screening of sleep‐promoting effects of human intestinal and food‐associated bacteria on Drosophila melanogaster

Neuromicrobiology, an emerging neurometabolic facet of the gut microbiome?

Probiotic Lactobacillus spp. improves Drosophila memory by increasing lactate dehydrogenase levels in the brain mushroom body neurons

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