Biogenic Amine Concentration in Testes of Rats at Different Ages

Zieher, Luis Marı́a; Debéljuk, Luciano; Iturriza, Fermín C.; Mancini, R. E.

doi:10.1210/endo-88-2-351

Cited by 47 publications

(28 citation statements)

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“…Taken together, the results of the present study and those reported by Prince and Tainio reveal a significant age-dependent change of adrenergic innervation of human testis. Although the mechanisms underlying such a change remain to be explored, there are several lines of evidence indicating that testicular adrenergic innervation is more important for the developing testes than for the fully functional adult ones (Zieher et al 1971;Nagai et al 1982; Anakwe & Fig. 3 VIP-IR nerves (black arrow) were observed in arterioles (L, large-sized arterioles; M, medium-sized arterioles) (A), but not in veins (V) (B).…”

Section: Discussionmentioning

confidence: 99%

Peptidergic not monoaminergic fibers profusely innervate the young adult human testis

Gong

Feng

et al. 2009

Journal of Anatomy

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Numerous studies have reported that intratesticular nerves exert important regulatory effects on the functions of the male gonad; however, as yet little is known about their distribution in the young adult human testis. The purpose of this study was to explore whether peptidergic and adrenergic nerves occur in the male gonad of this age, and, if present, to depict their distribution further. Thirty testes were collected from 15 reproductively healthy donors aged 21-32 years. Antibodies against protein gene product 9.5 (PGP 9.5), neuropeptide Y (NPY), C-terminal flanking peptide of NPY (CPON) and vasoactive intestinal peptide (VIP) were employed for immunohistochemical detection of intratesticular peptidergic nerves, and those against dopamine-beta-hydroxylase (DBH) and 5-hydroxytryptamine (5-HT) for monoaminergic ones. The testicular parenchyma exhibited a rich innervation by PGP 9.5-positive fibers, mainly associated with Leydig cell nests, blood vessels, and seminiferous tubules. Numerous NPY-and CPON-immunoreactive (IR) nerves also appeared in the gonads, but the vast majority were confined to blood vessels. A small number of VIP-IR fibers were detected in some arterioles. By contrast, however, no fibers displaying DBH or 5-HT immunoreactivity were observed within the testis. Additionally, expression of PGP-9.5, NPY, CPON, VIP, DBH and 5-HT was found in Leydig cells, PGP 9.5 in spermatogonia, and NPY and CPON in peritubular myoid cells. Our results suggest that the young adult human testis is devoid of monoaminergic nerves but profusely innervated by peptidergic fibers, which may serve as major neuronal regulators for testicular functions at this age.

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Section: Discussionmentioning

confidence: 99%

Peptidergic not monoaminergic fibers profusely innervate the young adult human testis

Gong

Feng

et al. 2009

Journal of Anatomy

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“…Since the concentration of testicular histamine is higher in the immature gonad than in the adult one (Zieher et al 1971), it can be assumed that the lower testicular weight observed in HDC-KO adult mice might be the consequence of histamine-dependent events during testicular development. This is supported by our finding that testicular weight is significantly reduced already in 7-day-old KO litters, at a time when the testes have not yet descended from the abdomen.…”

Section: Discussionmentioning

confidence: 99%

Histidine decarboxylase deficiency in gene knockout mice elevates male sex steroid production

Pap¹,

Rácz²,

Kovacs³

et al. 2002

Journal of Endocrinology

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Histamine is synthesized in cells by histidine decarboxylase (HDC). HDC-deficient knockout (KO) mice lack functional HDC and histamine in the tissues. In the present study we used this in vivo model for studying the role of HDC deficiency in the regulation of male steroid hormone metabolism.In agreement with earlier studies showing the lack of effects of central histamine on the basal secretion of gonadotrope hormones, we found no difference with in situ hybridization in the expression of GnRH in the hypothalamus of wild type and KO mice.The tissue concentrations of testosterone and several androgenic steroids were significantly elevated in the testes but not in the adrenal glands of HDC-KO mice. In contrast, serum estradiol levels failed to show a significant difference between the two groups. The weight of the testes was significantly smaller in both 7-day-old and adult KO mice. The ultrastructure of the adult testis indicated elevated steroid synthesis with more tightly coiled membranous whorls in Leydig cells.The present results suggest that changes in reproductive functions and sex steroid secretion in male HDC-KO mice are not due to altered hypothalamic GnRH expression but are probably related to definite modifications during fetal development of KO mice reinforced later by the lack of the effect of peripheral histamine. This may provide in vivo evidence that peripheral histamine is an important regulatory factor of male gonadal development during embryogenesis and of sex steroid metabolism later in adulthood.

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“…This drug has been shown to have the effect of blocking specifically the vascular permeability response to 5-hydroxytryptamine (5HT) in various other tissues in the rat, a species which is especially sensitive to this mediator (De Clerck et aL, 1985). Several authors have reported that the rat testis contains 5HT (Kormano and Penttila, 1968;Zieher et aL, 1971), although the claim by the latter authors that the highest concentrations were found in the testes of new-born rats was subsequently challenged (Lx)mbard-des Gouttes et aL, 1974). However, it is not clear from these reports whether the testicular artery was included in the tissue analysed, but it probably was not and the present finding of large numbers of mast cells in the vicinity of the testicular artery on the surface of the testis would suggest that high localized concentrations of 5HT may be produced under conditions of appropriate stimulation.…”

Section: Discussionmentioning

confidence: 99%

The Increase in Testicular Vascular Permeabilty Induced by Human Chorionic Gonadotrophin Involves 5‐hydroxytryptamine and Possibly Oestrogens, but Not Testosterone, Prostaglandins, Histamine or Bradykinin

Sowerbutts

Jarvis

Setchell

1986

Aust J Exp Biol Med

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Summary. Possible intermediates in the response of the rat testicular vasculature to human chorionic gonadotrophin (hCG) have been investigated. Ketanserin, an antagonist of 5-hydroxytryptamine, significantly reduced the increase in 1-h albumin space seen 20 h after hCG, as did one aromatase inhibitor (l,4,6-androstatriene-3, 17-dione), but the effect of another (testolactone) did not reach significance. Aminoglutethimide, which inhibits overall steroid synthesis as well as aromatase, reduced the albumin space in both control and hCG-injected rats but the hCG response, as judged by the ratio between treated and control rats, was unaffected. Inhibitors of overall steroid synthesis (WIN 32,729), prostaglandin synthesis (meclofenamic acid or indomethacin) and angiotensin converting enzyme (captopril) and blockers of HI and H2 histamine receptors (mepyramine, cimetidine or ranitidine) were without effect. The time course of the vascular response to hCG is quite different from the response in testosterone secretion by the testis. Considerable numbers of mast cells were found in the vicinity of the testicular artery in the testicular capsule, and these may be a source of 5-hydroxytryptamine. INTRODUCTIONRecently, it was shown that a single injection of hCG into a male rat produced a striking increase in the permeability of the testicular blood vessels to albumin, in the amount of extracellular fluid in the testis and in testicular lymph flow (Setchell and Sharpe, 1981). For several reasons, it was suggested in that paper that hCG was not acting directly on the blood vessels, but rather that the effect was due to some testicular product whose formation was increased by hCG. Two obvious possibilities were oestrogens and prostaglandins. The present paper reports the results of a series of experiments designed to establish whether these or other possible vasoactive substances are involved in the hCG effect. While this paper was being written, the results of an independent study were published (Veijola and Rajaniemi, 1985), confirming our observations on the lack of effect of indomethacin and aminoglutethimide.

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Biogenic Amine Concentration in Testes of Rats at Different Ages

Cited by 47 publications

References 0 publications

Peptidergic not monoaminergic fibers profusely innervate the young adult human testis

Peptidergic not monoaminergic fibers profusely innervate the young adult human testis

Histidine decarboxylase deficiency in gene knockout mice elevates male sex steroid production

The Increase in Testicular Vascular Permeabilty Induced by Human Chorionic Gonadotrophin Involves 5‐hydroxytryptamine and Possibly Oestrogens, but Not Testosterone, Prostaglandins, Histamine or Bradykinin

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