Biogenic amine receptors in parasitic nematodes: what can be learned from Caenorhabditis elegans?

Komuniecki, Richard; Hobson, Robert J.; Rex, Elizabeth; Hapiak, Vera; Komuniecki, Patricia R.

doi:10.1016/j.molbiopara.2004.05.010

Cited by 63 publications

(66 citation statements)

References 76 publications

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“…For example, after 15 min on 5-HT, ser-4;mod-1 and wild-type animals had laid 12.3 6 3.2 and 4.8 6 1.1 eggs, respectively, while after 60 min they had laid 13.6 6 2.6 and 11.7 6 1.8 eggs, respectively (Figure 1). Together, these results support earlier observations that SER-4 and MOD-1 provide inhibitory input into the egglaying process (Komuniecki et al 2004;Carnell et al 2005;Dempsey et al 2005). Surprisingly, although 5-HT did not stimulate egg laying in ser-1, ser-7 or ser-7 ser-1 animals, 5-HT still significantly stimulated egg laying in ser-4;mod-1;ser-7 ser-1 animals ( Figure 1 and Figure 2; Hobson et al 2006).…”

Section: Resultssupporting

confidence: 91%

“…The ser-4;mod-1;ser-7 ser-1 animals were viable, although they failed to respond to 5-HT in most locomotory, feeding, and behavioral assays (data not shown). All ser-4, mod-1 or ser-4;mod-1 null animals laid eggs more rapidly on 5-HT than wild-type animals, and this increased 5-HT responsiveness was more much apparent earlier in the egg-laying assays (Figure 1; data not shown; Komuniecki et al 2004). For example, after 15 min on 5-HT, ser-4;mod-1 and wild-type animals had laid 12.3 6 3.2 and 4.8 6 1.1 eggs, respectively, while after 60 min they had laid 13.6 6 2.6 and 11.7 6 1.8 eggs, respectively (Figure 1).…”

Section: Resultsmentioning

confidence: 91%

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Dual Excitatory and Inhibitory Serotonergic Inputs Modulate Egg Laying inCaenorhabditis elegans

Hapiak

Hobson²,

Hughes

et al. 2009

Genetics

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Serotonin (5-HT) regulates key processes in both vertebrates and invertebrates. Previously, four 5-HT receptors that contributed to the 5-HT modulation of egg laying were identified in Caenorhabditis elegans. Therefore, to assess potential receptor interactions, we generated animals containing combinations of null alleles for each receptor, especially animals expressing only individual 5-HT receptors. 5-HTstimulated egg laying and egg retention correlated well with different combinations of predicted excitatory and inhibitory serotonergic inputs. For example, 5-HT did not stimulate egg laying in ser-1, ser-7, or ser-7 ser-1 null animals, and ser-7 ser-1 animals retained more eggs than wild-type animals. In contrast, 5-HT-stimulated egg laying in ser-4;mod-1 animals was greater than in wild-type animals, and ser-4;mod-1 animals retained fewer eggs than wild-type animals. Surprisingly, ser-4;mod-1;ser-7 ser-1 animals retained the same number of eggs as wild-type animals and exhibited significant 5-HT-stimulated egg laying that was dependent on a previously uncharacterized receptor, SER-5. 5-HT-stimulated egg laying was absent in ser-5;ser-4;mod-1;ser-7 ser-1 animals, and these animals retained more eggs than either wild-type or ser-4;mod-1;ser-7 ser-1 animals. The 5-HT sensitivity of egg laying could be restored by ser-5 muscle expression. Together, these results highlight the dual excitatory/inhibitory serotonergic inputs that combine to modulate egg laying.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 91%

Dual Excitatory and Inhibitory Serotonergic Inputs Modulate Egg Laying inCaenorhabditis elegans

Hapiak

Hobson²,

Hughes

et al. 2009

Genetics

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“…Nematodes, like insects, lack adrenergic receptors, relying instead upon other biogenic amines and neuropeptides to control key physiological processes (Komuniecki et al, 2004). In the model C. elegans, there are at least 15 potential amine receptor candidates detected by BLAST comparison of the genome with a Drosophila 5-HT receptor.…”

Section: Discussionmentioning

confidence: 99%

A transporter for phenolamine uptake in the arthropod CNS

Donly

Caveney

2005

Arch Insect Biochem Physiol

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Biogenic monoamines play central roles in the nervous control of physiological processes in both vertebrates and invertebrates, each using a suite of neurotransmitters tailored through evolution. Among the ancillary proteins necessary for the deployment of monoamine transmitters are membrane-bound transporters that enable the reuptake of synaptically released transmitters. Transporters responsible for monoamine uptake include a novel transporter discovered in a pest insect, the cabbage looper Trichoplusia ni, which has high affinity for the phenolamines octopamine and tyramine. Sequence analysis suggests that this transporter has no direct ortholog in the sequenced genomes of model invertebrates. We report here a preliminary investigation into the true extent of the distribution of this type of transporter using RT-PCR with a set of degenerate primers selective for monoamine transporters on cDNAs made from the nervous systems of a range of arthropods. PCR products encoding the N-terminal region of orthologs of this transporter were detected in a variety of insect orders, as well as in a crustacean, but were not found in representatives of either the Diptera or the Hymenoptera. Thus, although this transporter is widely expressed in invertebrates, there are various invertebrates that appear to have evolved alternate ways of recycling phenolamine neurotransmitters released at the nerve synapse.

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“…Dopamine signaling also leads to more efficient searches for new food sources. After wild-type nematodes exhaust an immediate food supply, they continue to search the nearby area before expanding their exploration [79]. This localized search is referred to as "restricted searching" and is characterized by high-angled turns [61].…”

Section: Neurotransmitters: Dopaminementioning

confidence: 99%