Biogenic amines and hygienic quality of lucerne silage

Mlejnková, Veronika; Horký, Pavel; Komínková, Markéta; Skládanka, Jiří­; Hodulíková, Lucia; Adam, Vojtěch; Mlček, Jiří­; Jurí­ková, Tűnde; Sochor, Jiří­

doi:10.1515/biol-2016-0037

Cited by 9 publications

(6 citation statements)

References 22 publications

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“…Ash material was determined by weighing the material remaining after the burning of a fixed-weight sample at 550 °C and specified conditions. Nitrogenous substances were measured by the Kjeldal method (N × 6.25 coefficient) using the Kjeltec 2300 device (Foss Analytical, Hillerød, Denmark) [ 60 ].…”

Section: Methodsmentioning

confidence: 99%

Response of Normal and Low-Phytate Genotypes of Pea (Pisum sativum L.) on Phosphorus Foliar Fertilization

Škarpa

Školníková

Antošovský

et al. 2021

Plants

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Phosphorus (P) is an important nutrient in plant nutrition. Its absorption by plants from the soil is influenced by many factors. Therefore, a foliar application of this nutrient could be utilized for the optimal nutrition state of plants. The premise of the study is that foliar application of phosphorus will increase the yield of normal-phytate (npa) cultivars (CDC Bronco a Cutlass) and low-phytate (lpa) lines (1-2347-144, 1-150-81) grown in soils with low phosphorus supply and affect seed quality depending on the ability of the pea to produce phytate. A graded application of phosphorus (H₃PO₄) in four doses: without P (P0), 27.3 mg P (P1), 54.5 mg P (P2), and 81.8 mg P/pot (P3) realized at the development stages of the 6th true leaf led to a significant increase of chlorophyll contents, and fluorescence parameters of chlorophyll expressing the CO2 assimilation velocity. The P fertilization increased the yield of seeds significantly, except the highest dose of phosphorus (P3) at which the yield of the npa cultivars was reduced. The line 1-2347-144 was the most sensible to the P application when the dose P3 increased the seed production by 42.1%. Only the lpa line 1-150-81 showed a decreased tendency in the phytate content at the stepped application of the P nutrition. Foliar application of phosphorus significantly increased ash material in seed, but did not tend to affect the protein and mineral content of seeds. Only the zinc content in seeds was significantly reduced by foliar application of P in npa and lpa pea genotypes. It is concluded from the present study that foliar phosphorus application could be an effective way to enhance the pea growth in P-deficient condition with a direct effect on seed yield and quality.

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Section: Methodsmentioning

confidence: 99%

Response of Normal and Low-Phytate Genotypes of Pea (Pisum sativum L.) on Phosphorus Foliar Fertilization

Škarpa

Školníková

Antošovský

et al. 2021

Plants

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“…Maize forage compaction ranged from 155 to 198 kg TS /m 3 (Table 2). Then, the mini-silo was hermetically sealed and placed in the dark to prevent exposure to light, at a temperature of 28 • C ± 1 • C. Each container was equipped with a safety valve for the removal of excessive gaseous products [28,29]. After the lapse of the incubation time of 90 days [29], the micro-silages were opened and samples of them were homogenized.…”

Section: Production Of Mixed Culture Silage-preparation Of Model Silagementioning

confidence: 99%

Possibilities of Using White Sweetclover Grown in Mixture with Maize for Biomethane Production

Kintl¹,

Elbl

Vítěz

et al. 2020

Agronomy

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Methods of growing plant biomass for the production of biogas in anaerobic digestion plants have a decisive influence on arable land and on the evaluation of biogas plant technologies from the environmental point of view. The main benefit of anaerobic digestion is the possibility to use various agricultural crops for energy production. Some of these plant species, e.g., legumes, are generally considered to be beneficial for arable soil quality, as compared with maize monocultures with frequently manifested soil degradation and adverse environmental impact on arable land. A possible change is offered by cultivation systems composed of two and more crops and defined as mixed cropping (MC) systems. The systems are characterized by a more efficient utilization of natural resources of the site as well as by a greater potential for arable soil protection. A question remains as to whether the MC system of growing maize and white sweetclover can be used for biogas yield. In the presented research study, a mixed cropping system was tested with maize (Zea mays L.) and white sweetclover (Melilotus albus MED.). The goal of our research was to determine an optimum ratio of maize and white sweetclover (s.c.) shreddings in silage for a biogas plant. For this purpose, model micro-silages of monocultures were prepared: maize (100%), white s.c. (100%), as well as variants with different weight shares of these two crops (maize:white s.c.; 3:7, 1:1, 7:3, 8:2, 8.5:1.5, 9:1). The silages were subjected to biomethanation tests, in order to determine the influence of the increased addition of white s.c. biomass on methane yield and methane concentration in biogas. The highest values of biogas yield were recorded in the maize monoculture and in the MC variant of maize and white s.c. at 9:1 (>0.26 m3/kgVS). The lowest methane yield values were recorded in the white s.c. monoculture (0.16 m3/kgVS). It was found out that the yield of methane decreased with an increasing share of white sweetclover in the maize silage, due to the increased content of poorly degradable organic substances and the presence of fermentation inhibitors (e.g., coumarin).

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“…Numerous studies suggest that the occurrence of NH 3 and BAEs also exists in substantially well‐preserved silages (Mlejnkova et al, 2016; Scherer et al, 2015; Skladanka et al, 2017), but available studies merely focused on the effect of different additives on the concentrations of NH 3 and BAEs, whilst information on functional micro‐organisms and active mechanisms remain mostly undescribed. Our previous findings showed that sucrose addition could effectively improve the fermentation quality of alfalfa silage (Li, et al, 2020; Zheng et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis reveals the linkages between bacteria and the functional enzymes responsible for potential ammonia and biogenic amine production in alfalfa silage

Zheng

Cai

et al. 2022

Journal of Applied Microbiology

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Aims:To clarify the molecular mechanisms underlying ammonia (NH 3 ) and biogenic amines (BAEs) formation in alfalfa silage, whole metagenomic sequencing analysis was performed to identify the linkages between functional bacteria and their responsible enzymes in alfalfa silage prepared with and without sucrose addition. Methods and Results:Genes encoding nitrite reductase (nirB) resulting in NH 3 formation were the most abundant and were mostly assigned to Enterobacter cloacae and Klebsiella oxytoca. Putrescine-related genes, classified mainly to encode ornithine decarboxylase (odcA), were predominantly carried by Escherichia coli, Ent. cloacae and Citrobacter sp. Escherichia coli and Kl. oxytoca were the important species responsible for cadaverine and tyramine formation. Ent. cloacae, E. coli, and Kl. oxytoca dominated the bacterial community in naturally fermented alfalfa silage, whilst sucrose-treated silages greatly inhibited the growth of these species by promoting the dominance of Lactobacillus plantarum, thus decreasing the concentrations of NH 3 , cadaverine, putrescine and tyramine.Conclusions: Enterobacteriaceae bacteria are mainly responsible for the NH 3 , putrescine, cadaverine and tyramine formations in alfalfa silage. Significance and Impact of the Study:Whole metagenomic sequencing analysis served as a useful tool to identify the linkages between functional bacteria and associated enzymes responsible for NH 3 and BAEs formation.

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Biogenic amines and hygienic quality of lucerne silage

Cited by 9 publications

References 22 publications

Response of Normal and Low-Phytate Genotypes of Pea (Pisum sativum L.) on Phosphorus Foliar Fertilization

Response of Normal and Low-Phytate Genotypes of Pea (Pisum sativum L.) on Phosphorus Foliar Fertilization

Possibilities of Using White Sweetclover Grown in Mixture with Maize for Biomethane Production

Metagenomic analysis reveals the linkages between bacteria and the functional enzymes responsible for potential ammonia and biogenic amine production in alfalfa silage

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