Biogenic antimicrobial silver nanoparticles produced by fungi

Abstract

Aspergillus tubingensis and Bionectria ochroleuca showed excellent extracellular ability to synthesize silver nanoparticles (Ag NP), spherical in shape and 35 ± 10 nm in size. Ag NP were characterized by transmission electron microscopy, X-ray diffraction analysis, and photon correlation spectroscopy for particle size and zeta potential. Proteins present in the fungal filtrate and in Ag NP dispersion were analyzed by electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Ag NP showed pronounced antifungal activity against Candida sp, frequently occurring in hospital infections, with minimal inhibitory concentration in the range of 0.11-1.75 μg/mL. Regarding antibacterial activity, nanoparticles produced by A. tubingensis were more effective compared to the other fungus, inhibiting 98.0 % of Pseudomonas. aeruginosa growth at 0.28 μg/mL. A. tubingensis synthesized Ag NP with surprisingly high and positive surface potential, differing greatly from all known fungi. These data open the possibility of obtaining biogenic Ag NP with positive surface potential and new applications.

Biosynthesis and Characterization of Titanium Dioxide Nanoparticles Using Pithecellobium Dulce and Lagenaria Siceraria Aqueous Leaf Extract and Screening their Free Radical Scavenging and Antibacterial Properties

“…Biological route synthesis of nanoparticles has received much focused attention from researchers in order to elucidate the mechanism of synthesis. To circumvent this many biological systems like bacteria [Kumar CG et al, 2011], fungi [Rodriques AG et al, 2012], yeast, cyanobacteria, actinomycetes and plants have been used. But the best one appears to be the use of plants.…”

Mycofabrication of common plasmonic colloids, theoretical considerations, mechanism and potential applications

“…Both mycogenerated particles were spherical with the sizes between 25 nm and 45 nm. These fungal AgNPs showed to be highly effective against human pathogen Candida sp., frequently occurring in hospital infections [136].…”

“…al. [136] from the Mangrove plant and their cell-free media used to synthesise AgNPs. While nanoparticles were produced rapidly by both fungi, Bionectria ochroleucaI produced medium was more efficient than that of Aspergillus tubingensis.…”

ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in <em>Saccharomyces cerevisiae</em>

“…9,10 Nanoparticles biosynthesized by organisms, without involving the toxic solvents, strict conditions, or expensive processes that are needed in chemical methods, have become a popular subject. 11 Various nanoparticles are reported that are biosynthesized by bacteria, [12][13][14] fungi, 15,16 plants, 17,18 mammalian cells, 19 or even earthworms. 20 Considering the simple culture methods, the low cost of the equipment, and the easy way to get the gradients, the biosynthesis of nanoparticles using microorganisms is the most economic and common approach.…”