Biogenic concrete protection driven by the formate oxidation by Methylocystis parvus OBBP

Ganendra, Giovanni; Wang, Jianyun; Ramos, J.A.; Derluyn, Hannelore; Rahier, Hubert; Cnudde, Veerle; Ho, Adrian; Boon, Nico

doi:10.3389/fmicb.2015.00786

Cited by 18 publications

(8 citation statements)

References 46 publications

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“…In addition, our results for H. hauckii DDAs found no evidence of growth rate photoinhibition at the highest light level used (500 µmol m −2 s −1 ). While instantaneous solar PAR may reach ~2,000 µmol m −2 s −1 ( Björkman et al, 2015 ) at Station ALOHA near Hawaii (22° 45′ N 158° 00′ W), average daily PAR incident at Sta. ALOHA over the diurnal is ~850 µmol m −2 s −1 from June to August (calculated from Letelier et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Isolation, growth, and nitrogen fixation rates of the Hemiaulus-Richelia (diatom-cyanobacterium) symbiosis in culture

Pyle

Johnson

Villareal

2020

PeerJ

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Nitrogen fixers (diazotrophs) are often an important nitrogen source to phytoplankton nutrient budgets in N-limited marine environments. Diazotrophic symbioses between cyanobacteria and diatoms can dominate nitrogen-fixation regionally, particularly in major river plumes and in open ocean mesoscale blooms. This study reports the successful isolation and growth in monocultures of multiple strains of a diatom-cyanobacteria symbiosis from the Gulf of Mexico using a modified artificial seawater medium. We document the influence of light and nutrients on nitrogen fixation and growth rates of the host diatom Hemiaulus hauckii Grunow together with its diazotrophic endosymbiont Richelia intracellularis Schmidt, as well as less complete results on the Hemiaulus membranaceus-R. intracellularis symbiosis. The symbioses rates reported here are for the joint diatom-cyanobacteria unit. Symbiont diazotrophy was sufficient to support both the host diatom and cyanobacteria symbionts, and the entire symbiosis replicated and grew without added nitrogen. Maximum growth rates of multiple strains of H. hauckii symbioses in N-free medium with N2 as the sole N source were 0.74–0.93 div d−1. Growth rates followed light saturation kinetics in H. hauckii symbioses with a growth compensation light intensity (EC) of 7–16 µmol m−2s−1and saturation light level (EK) of 84–110 µmol m−2s−1. Nitrogen fixation rates by the symbiont while within the host followed a diel pattern where rates increased from near-zero in the scotophase to a maximum 4–6 h into the photophase. At the onset of the scotophase, nitrogen-fixation rates declined over several hours to near-zero values. Nitrogen fixation also exhibited light saturation kinetics. Maximum N2 fixation rates (84 fmol N2 heterocyst−1h−1) in low light adapted cultures (50 µmol m−2s−1) were approximately 40–50% of rates (144–154 fmol N2 heterocyst−1h−1) in high light (150 and 200 µmol m−2s−1) adapted cultures. Maximum laboratory N2 fixation rates were ~6 to 8-fold higher than literature-derived field rates of the H. hauckii symbiosis. In contrast to published results on the Rhizosolenia-Richelia symbiosis, the H. hauckii symbiosis did not use nitrate when added, although ammonium was consumed by the H. hauckii symbiosis. Symbiont-free host cell cultures could not be established; however, a symbiont-free H. hauckii strain was isolated directly from the field and grown on a nitrate-based medium that would not support DDA growth. Our observations together with literature reports raise the possibility that the asymbiotic H. hauckii are lines distinct from an obligately symbiotic H. hauckii line. While brief descriptions of successful culture isolation have been published, this report provides the first detailed description of the approaches, handling, and methodologies used for successful culture of this marine symbiosis. These techniques should permit a more widespread laboratory availability of these important marine symbioses.

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Section: Discussionmentioning

confidence: 99%

Isolation, growth, and nitrogen fixation rates of the Hemiaulus-Richelia (diatom-cyanobacterium) symbiosis in culture

Pyle

Johnson

Villareal

2020

PeerJ

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“…Few anaerobic methane-oxidizing microorganisms have been isolated from diverse environments such as Kidd mud volcano in the gulf, salt dome cap rocks and Tuscan archipelago and the mechanism of bicarbonate formation via methane oxidation was evaluated [34,35,36]. Also, as reported in other studies, Methylo cystisparvus microbe was employed and optimized for the calcium carbonate biomineral formation to produce environment-friendly building materials via the methane oxidation mineralization process [37][38][39]. Although this metabolic pathway is well-described and carried out under laboratory conditions, implementing it in prevailing in situ environmental conditions demands further research and analysis.…”

Section: Methane Oxidationmentioning

confidence: 99%

A critical review on microbial carbonate precipitation via denitrification process in building materials

2021

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The naturally occurring biomineralization or microbially induced calcium carbonate (MICP) precipitation is gaining huge attention due to its widespread application in various fields of engineering. Microbial denitrification is one of the feasible metabolic pathways, in which the denitrifying microbes lead to precipitation of carbonate biomineral by their basic enzymatic and metabolic activities. This review article explains all the metabolic pathways and their mechanism involved in the MICP process in detail along with the benefits of using denitrification over other pathways during MICP implementation. The potential application of denitrification in building materials pertaining to soil reinforcement, bioconcrete, restoration of heritage structures and mitigating the soil pollution has been reviewed by addressing the finding and limitation of MICP treatment. This manuscript further sheds light on the challenges faced during upscaling, real field implementation and the need for future research in this path. The review concludes that although MICP via denitrification is an promising technique to employ it in building materials, a vast interdisciplinary research is still needed for the successful commercialization of this technique.

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“…The technique may also be used to quantify the amount of healing (volume of healing products and distribution along the crack) next to qualitatively studying the healing products . In this way, solid conclusions may be drawn on the amount of autogenous healing capacity in the interior of specimens, the amount of bacterial calcium carbonate crystallization or precipitation by a bacterial treatment or the amount of polymerization of polyurethanes from tubular capsules . The healing can also be studied as a function of time by subsequent imposed healing cycles or water curing .…”

Section: Healing Efficiencymentioning

confidence: 99%

Validation of Self‐Healing Properties of Construction Materials through Nondestructive and Minimal Invasive Testing

Snoeck

Malm

Cnudde

et al. 2018

Adv Materials Inter

Self Cite

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When studying the self‐healing properties of construction materials, a plethora of destructive and nondestructive testing (NDT) techniques can be used. In this review, the applicability of different nondestructive test methods is discussed in detail. The methods can be categorized whether they are used to study the encapsulation and/or protection mechanism of the healing agent, the sequestered healing agent itself, the distribution of healing agents, the trigger mechanism for healing, the healing efficiency, or healing performance. Based on this categorization, nondestructive techniques found in literature are discussed. In this way, a robust understanding of the different techniques can be used for future research on self‐healing construction materials.

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Biogenic concrete protection driven by the formate oxidation by Methylocystis parvus OBBP

Cited by 18 publications

References 46 publications

Isolation, growth, and nitrogen fixation rates of the Hemiaulus-Richelia (diatom-cyanobacterium) symbiosis in culture

Isolation, growth, and nitrogen fixation rates of the Hemiaulus-Richelia (diatom-cyanobacterium) symbiosis in culture

A critical review on microbial carbonate precipitation via denitrification process in building materials

Validation of Self‐Healing Properties of Construction Materials through Nondestructive and Minimal Invasive Testing

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