Biogenic monoamines in mouse oocytes and preimplantation embryos

Sadykova, K. A.; Markova, L. N.; Baikenova, S. D.; Vsevolodov, E. B.; Buznikov, G. A.

doi:10.1007/bf00841442

Cited by 3 publications

(3 citation statements)

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“…The possible sources of norepinephrine and epinephrine in the oviductal fluid could be nerves, blood circulation or graafian follicles (Helm et al 1982, Fernandez-Pardal et al 1986, Kannisto et al 1986, Itoh et al 2000, Kotwica et al 2003. Moreover, it was speculated that catecholamines could be produced also by the early embryo itself (Burden & Lawrence 1973, Sadykova et al 1990). Epinephrine and norepinephrine present in oviductal fluid could influence the oviduct epithelium via adrenergic receptors which have been shown in oviduct epithelial cells of several species (Tolszczuk & Pelletier 1988, Dickens et al 1993, Einspanier et al 1999.…”

Section: Discussionmentioning

confidence: 99%

Expression of adrenergic receptors in mouse preimplantation embryos and ovulated oocytes

Číkoš

Rehák²,

Czikková

et al. 2007

Reproduction

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Epinephrine and norepinephrine can play an important role in basic developmental processes such as embryogenesis and morphogenesis, regulating cell proliferation, differentiation and migration. We showed that b-adrenergic receptors can mediate the effects of catecholamines on preimplantation embryos in our previous work. In the present study, we designed specific oligonucleotide primers which can distinguish among all members of the a-adrenergic receptor family, and showed (using RT-PCR) that the a2C-adrenergic receptor is transcribed in ovulated oocytes, 8-to 16-cell morulae and expanded blastocysts. We did not detect the a2C-adrenoceptor transcript in 4-cell embryos. Our immunohistochemical study showed the presence of a-2C-adrenoceptor protein in ovulated oocytes, 8-to 16-cell embryos and blastocysts, but the signal in 4-cell embryos was weak, and probably represents remaining protein of maternal origin. We did not detect any other a-adrenergic receptor in preimplantation embryos and oocytes. Exposure of mouse preimplantation embryos to the a2-adrenergic agonist UK 14 304 led to significant reduction of the embryo cell number, and the effect was dose dependent. Our results suggest that epinephrine and norepinephrine could affect the embryo development in the oviduct via adrenergic receptors directly and support the opinion that maternal stress can influence the embryo even in very early pregnancy.

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Section: Discussionmentioning

confidence: 99%

Expression of adrenergic receptors in mouse preimplantation embryos and ovulated oocytes

Číkoš

Rehák²,

Czikková

et al. 2007

Reproduction

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“…On the other hand, it has been shown that addition of beta adrenoceptors antagonists (alprenolol, propranolol) to mouse preimplantation embryos in vitro can inhibit cell proliferation and induce developmental anomalies, suggesting that beta adrenoceptors agonists may be produced by the embryo itself to influence developmental processes . The possibility that catecholamines could be produced by the early embryo itself has been suggested for the mouse, rat, and frog (Burden and Lawrence, 1973;Sadykova et al, 1990;Devic et al, 1997). Another possible explanation for the effects of beta adrenoceptors antagonists on mouse preimplantation embryos is that the antagonists could produce effects that mimic those elicited by agonists.…”

Section: Discussionmentioning

confidence: 99%

“…Production of catecholamines as well as expression of adrenergic receptors in mouse and rat fetus has been shown in several studies (Slotkin et al, 1994;Kobayashi et al, 1995;Thomas et al, 1995;Zhou et al, 1995;Fujinaga and Scott, 1997;Wang and Limbird, 1997;Ebert and Thompson, 2001). Moreover, there are results suggesting that catecholamines can influence the mouse embryo even in the very early stages of development-before its implantation into the uterus Sadykova et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Expression of beta adrenergic receptors in mouse oocytes and preimplantation embryos

et al. 2005

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Accumulating evidence indicates the role of endogenous catecholamines in mammalian embryogenesis. We searched public databases containing nucleotide sequences derived from mouse preimplantation cDNA libraries and found a partial sequence homology between a cDNA clone from mouse blastocysts and the mouse beta 2-adrenergic receptor sequence. No significant sequence homology was found for other mouse adrenergic and dopamine receptors. Using RT-PCR, we showed that beta 2-adrenoceptor is transcribed not only at blastocyst stage but also at earlier stages of preimplantation development as well as in oocytes. Moreover, we demonstrated that transcripts encoding both isoforms of the beta 3-adrenoceptor (beta 3a- and beta 3b-) are expressed in mouse oocytes and preimplantation embryos as well. We did not detect the beta 1-adrenoceptor transcript either in oocytes or in preimplantation embryos. Using an antibody against the mouse beta 2-adrenergic receptor, we showed that the receptor protein is expressed in oocytes and preimplantation embryos; in blastocysts, the immufluorescence labeling was stronger in the inner cell mass than in throphectodermal cells. The cell number of the in vitro cultured mouse preimplantation embryos exposed to isoproterenol (a potent beta adrenoceptor agonist) was lower than in control embryos, suggesting that activation of beta adrenergic receptors by appropriate agonist concentration can influence cell proliferation in mouse pre-implantation embryos. Thus, our results indicate that beta adrenergic receptors are expressed in mouse oocytes and preimplantation embryos and that ligands for the receptors can affect the mouse embryo even in the very early stages of development.

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Developmental regulation of catecholamine levels during sea urchin embryo morphogenesis

Anitole-Misleh

Brown

2004

Comparative Biochemistry and Physiology Part A: Molecular &

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Biogenic monoamines in mouse oocytes and preimplantation embryos

Cited by 3 publications

References 5 publications

Expression of adrenergic receptors in mouse preimplantation embryos and ovulated oocytes

Expression of adrenergic receptors in mouse preimplantation embryos and ovulated oocytes

Expression of beta adrenergic receptors in mouse oocytes and preimplantation embryos

Developmental regulation of catecholamine levels during sea urchin embryo morphogenesis

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