Biogenic nanoparticles: Synthesis, stability and biocompatibility mediated by proteins of Pseudomonas aeruginosa

Quinteros, Melisa de Los Ángeles; Bonilla, José Oscar; Alborés, Silvana; Villegas, Liliana Beatriz; Páez, Paulina Laura

doi:10.1016/j.colsurfb.2019.110517

Cited by 34 publications

(12 citation statements)

References 39 publications

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“…The use of fungi for silver nanoparticle production provides improved production capacity due to the high quantities of secreted substances; these bioactive substances play a dual functional role as reducing and stabilizing agents to the colloidal nanoparticles [21]. Confocal Raman Microscopy was used to characterize the surface functional groups capping of the silver nanoparticles ( Figure 5).…”

Section: Confocal Raman Microscopy: Surface Functional Groups Of Pchnpsmentioning

confidence: 99%

Biofilm Eradication Using Biogenic Silver Nanoparticles

et al. 2020

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Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of Phanerochaete chrysosporium, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45–nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to −30 mV and showed colloidal stability from pH 3–9 and under conditions of high ionic strength ([NaCl] = 10–500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-E. coli interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of E. coli cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of E. coli and C. albicans biofilms.

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Section: Confocal Raman Microscopy: Surface Functional Groups Of Pchnpsmentioning

confidence: 99%

Biofilm Eradication Using Biogenic Silver Nanoparticles

et al. 2020

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“…In particular, due to the sterilization ability, [ 1 , 2 ] the research and application of AgNPs in biological systems has received widespread attention [ 3 , 4 ]. As we all know, NPs are enshrouded by a layer of biomolecules after being exposed to a biological matrix, generating a novel interface called ‘protein corona’, thus changing the physicochemical properties of NPs to influence their biological effect [ 5 , 6 ]. In general, the competitive binding between NPs and proteins is a dynamic process.…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic and Kinetic Binding Behaviors of Human Serum Albumin to Silver Nanoparticles

2022

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A nanoparticle, under biological milieu, is inclined to be combined with various biomolecules, particularly protein, generating an interfacial corona which provides a new biological identity. Herein, the binding interaction between silver nanoparticles (AgNPs) and human serum albumin (HSA) was studied with transmission electron microscopy (TEM), circular dichroism (CD), and multiple spectroscopic techniques. Due to the ground state complex formed mainly through hydrophobic interactions, the fluorescence titration method proved that intrinsic fluorescence for HSA was probably statically quenched by AgNPs. The complete thermodynamic parameters were derived, indicating that the interaction between HSA and AgNPs is an entropy-driven process. Additionally, synchronous fluorescence and CD spectrum results suggested the conformational variation it has upon binding to AgNPs and the α-helix content has HSA visibly decreased. The kinetic experiments proved the double hysteresis effect has in HSA’s binding to the AgNPs surface. Moreover, the binding has between HSA and AgNPs follows the pseudo-second-order kinetic characteristic and fits the Freundlich model for multilayer adsorption. These results facilitate the comprehension about NPs’ underlying biological effects under a physiological environment and promote the secure applications of NPs biologically and medically.

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“…Noteworthy that the AgNPs synthesized from bacterial strains showed greater antibacterial activity in comparison with metallic nanoparticles synthesized by physicochemical methods, due to the presence of proteins that can interact with biomolecules, resulting in greater biocompatibility, which can act mainly in cell lysis, through a change in membrane potential, loss of cytoplasmic material and inhibition of the respiratory chain [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%