Biogenic phytochemicals (cassinopin and isoquercetin) capped copper nanoparticles (ISQ/CAS@CuNPs) inhibits MRSA biofilms

Lotha, Robert; Shamprasad, Bhanuvalli R.; Sundaramoorthy, Niranjana Sri; Nagarajan, Saisubramanian; Sivasubramanian, Aravind

doi:10.1016/j.micpath.2019.05.005

Cited by 50 publications

(15 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S5 A) it was observed that bactericidal effects of both AgNPs and CuNPs were almost as similar to standard over a period of 24 h. From the alizarin test, it was confirmed that biogenic CuNPs release their cations (Cu + ) into the growth medium (Fig. S5 B) and these Cu + ions using the electrostatic attraction stick on the cell wall of the bacterial cells or sometimes, these CuNPs can penetrate the bacteria through the membrane and lead to cell death 63 , 64 .…”

Section: Discussionmentioning

confidence: 78%

Metal nanoparticles functionalized with nutraceutical Kaempferitrin from edible Crotalaria juncea, exert potent antimicrobial and antibiofilm effects against Methicillin-resistant Staphylococcus aureus

Shamprasad

Lotha

Nagarajan

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Kaempferitrin (KF), a flavonol glycoside, was isolated from the edible plant Crotalaria juncea. Optimization for the synthesis of silver (AgNPs) and copper (CuNPs) nanoparticles using C. juncea extract and kaempferitrin were attempted for the first time. A detailed study on size and stability analysis have been reported. Efficacy of KF@AgNPs and KF@CuNPs against biofilm formation and planktonic mode of growth on methicillin-resistant Staphylococcus aureus (MRSA) along with possible mechanisms has been explored. Release of Cu(II) upon prolonged treatment with KF@CuNPs in the presence of MRSA was quantified through Alizarin red test, indicating the antibacterial effect is initiated by the CuNPs itself. Time kill curve depicted both the NPs have similar kill kinetics to curtail the pathogen and imaging with Crystal violet assay, Fluorescent live dead imaging and SEM analysis revealed a 60% reduction in biofilm formation at the Sub-MIC concentration of KF@AgNPs and KF@CuNPs. Furthermore, the membrane permeability and cell surface hydrophobicity were altered in the presence of both the NPs. The colony count from the in vivo infection zebrafish model in the treatment group showed a decline of > 1.8 fold for KF@AgNPs and > two fold for KF@CuNPs. Toxicity studies did not reveal any abnormality in liver and brain enzyme levels. Liver morphology images show no severe cytological alterations when treated with KF@AgNPs and were almost similar to the normal liver. Thus, KF@AgNPs was nontoxic and caused significant reduction in biofilm formation in MRSA, also reduced bacterial bioburden in the infected zebrafish, which has the potential to be explored in higher animal models.

show abstract

Section: Discussionmentioning

confidence: 78%

Metal nanoparticles functionalized with nutraceutical Kaempferitrin from edible Crotalaria juncea, exert potent antimicrobial and antibiofilm effects against Methicillin-resistant Staphylococcus aureus

Shamprasad

Lotha

Nagarajan

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…CuO NPs (11.4–14.5 nm) fabricated using the leaf extract of Cymbopogon citratus suppressed the survival of MRSA, MSSA and E. coli , caused morphological deformations of bacterial cells, in which accumulation of Cu was observed; a dose of 2000 μg CuO NPs/mL resulted in the 49.0 ± 3.1% and 33.0 ± 3.2% inhibition of biofilm produced by MRSA and E. coli [ 297 ]. CuNPs capped with isoquercetin and cassinopin glycosides from Crotalaria candicans exhibited >50% reduction in biofilm formation by MRSA, suggesting that for antibiofilm activity the NPs predominantly alter membrane permeability and reduce surface hydrophobicity and the released Cu 2+ ions are not responsible for this [ 298 ]. A lipopeptide produced by human skin bacterium Paenibacillus thiaminolyticus and CuNPs/CuO NPs encapsulated in multilamellar liposomes exhibited pronounced reduction of the growth of MRSA and P. aeruginosa along with superb antibiofilm activity via their adverse impact on the cell metabolism, secreted virulence such as staphyloxanthin, pyocyanin, and extracellular polysaccharides [ 299 ].…”

Section: Applied Nanomaterialsmentioning

confidence: 99%

Advances in Nanostructures for Antimicrobial Therapy

Jampílek

Kráľová

2022

Materials

View full text Add to dashboard Cite

Microbial infections caused by a variety of drug-resistant microorganisms are more common, but there are fewer and fewer approved new antimicrobial chemotherapeutics for systemic administration capable of acting against these resistant infectious pathogens. Formulation innovations of existing drugs are gaining prominence, while the application of nanotechnologies is a useful alternative for improving/increasing the effect of existing antimicrobial drugs. Nanomaterials represent one of the possible strategies to address this unfortunate situation. This review aims to summarize the most current results of nanoformulations of antibiotics and antibacterial active nanomaterials. Nanoformulations of antimicrobial peptides, synergistic combinations of antimicrobial-active agents with nitric oxide donors or combinations of small organic molecules or polymers with metals, metal oxides or metalloids are discussed as well. The mechanisms of actions of selected nanoformulations, including systems with magnetic, photothermal or photodynamic effects, are briefly described.

show abstract

“…Rasool et al, (2019) studied the antibiofilm activity against fluconazole resistant Candida albicans biofilm. Lotha et al, (2019) studied about Cu-NPs capped with biogenic phytochemicals like cassinopin as well as isoquercitin and their antibiofilm effect against MRSA. Elsayed et al, (2020) found that disinfectant loaded Cu-NPs could be used for Staphylococcal biofilm eradication in poultry farm as well as in abattoirs.…”

Section: Copper Nanoparticlesmentioning

confidence: 99%

Metal Based Nanoparticles, a Novel Approach to Control Biofilm: Review

Das¹

2020

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Biogenic phytochemicals (cassinopin and isoquercetin) capped copper nanoparticles (ISQ/CAS@CuNPs) inhibits MRSA biofilms

Cited by 50 publications

References 44 publications

Metal nanoparticles functionalized with nutraceutical Kaempferitrin from edible Crotalaria juncea, exert potent antimicrobial and antibiofilm effects against Methicillin-resistant Staphylococcus aureus

Metal nanoparticles functionalized with nutraceutical Kaempferitrin from edible Crotalaria juncea, exert potent antimicrobial and antibiofilm effects against Methicillin-resistant Staphylococcus aureus

Advances in Nanostructures for Antimicrobial Therapy

Metal Based Nanoparticles, a Novel Approach to Control Biofilm: Review

Contact Info

Product

Resources

About