Biogenic Synthesis of Chitosan/Silver Nanocomposite by Escherichia coli D8 (MF062579) and its Antibacterial Activity

doi:10.54319/jjbs/160212

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…The biological synthesis of NPs is preferable due to its low cost, simplicity, safety, increased biocompatibility and stability, and non-toxic, high-productivity method for the production of NPs [ 23 ]. The biological method depends on the green chemistry that can be performed by living organisms or their natural secondary metabolites such as proteins including plants [ 24 , 25 ], fungi [ 26 ], yeast [ 27 ], algae [ 28 ], non-living viruses [ 29 ] and bacteria [ 23 , 30 , 31 ]. Among the significant categories of microorganisms involved in biosynthesis of NPs, the probiotic lactic acid bacteria (LAB) are recommended as a safe, rapid, easy to culture, available in dairy products and cheap bio-nano-factory for NPs production [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Anticandidal applications of selenium nanoparticles biosynthesized with Limosilactobacillus fermentum (OR553490)

Mohamed,

El‑Zahed

2024

Discover Nano

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Candida albicans is one of the most dangerous pathogenic fungi in the world, according to the classification of the World Health Organization, due to the continued development of its resistance to currently available anticandidal agents. To overcome this problem, the current work provided a simple, one-step, cost-effective, and safe technique for the biosynthesis of new functionalized anticandidal selenium nanoparticles (Se NPs) against C. albicans ATCC10231 using the cell-free supernatant of Limosilactobacillus fermentum (OR553490) strain. The bacterial strain was isolated from yogurt samples available in supermarkets, in Damietta, Egypt. The mixing ratio of 1:9 v/v% between cell-free bacterial metabolites and sodium selenite (5 mM) for 72 h at 37 °C were the optimum conditions for Se NPs biosynthesis. Ultraviolet–visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), Zeta analyses, and elemental analysis system (EDS) were used to evaluate the optimized Se NPs. The Se NPs absorption peak appeared at 254 nm. Physicochemical analysis of Se NPs revealed the crystalline-shaped and well-dispersed formation of NPs with an average particle size of 17–30 nm. Se NPs have − 11.8 mV, as seen by the zeta potential graph. FT-IR spectrum displayed bands of symmetric and asymmetric amines at 3279.36 cm−1 and 2928.38 cm−1, aromatic and aliphatic (C–N) at 1393.32 cm−1 and 1237.11.37 cm−1 confirming the presence of proteins as stabilizing and capping agents. Se NPs acted as a superior inhibitor of C. albicans with an inhibition zone of 26 ± 0.03 mm and MIC value of 15 µg/mL compared to one of the traditional anticandidal agent, miconazole, which revealed 18 ± 0.14 mm and 75 µg/mL. The cytotoxicity test shows that Se NPs have a low toxic effect on the normal keratinocyte (IC50 ≈ 41.5 μg/mL). The results indicate that this green synthesis of Se NPs may have a promising potential to provide a new strategy for drug therapy.

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Section: Introductionmentioning

confidence: 99%

Anticandidal applications of selenium nanoparticles biosynthesized with Limosilactobacillus fermentum (OR553490)

Mohamed,

El‑Zahed

2024

Discover Nano

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Antimicrobial Activity of N‐Substituted Derivatives of 5‐Chloro‐2,3‐ Diphenyl Indole

Sofan,

El‐Ablack,

Elsayed

2023

ChemistrySelect

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The indole motif plays a significant role in the quest for innovative drugs. As proposed, novel N‐substituted indole derivatives were synthesized, and their efficacy against bacteria and fungi was evaluated in vitro. The synthesis of these compounds was confirmed using easily accessible building blocks, and their structural formulas were supported by corrected analytical and spectroscopic analyses. Fresh strains of Candida albicans, Escherichia coli, and Pseudomonas aeruginosa, as well as Bacillus subtilis and Staphylococcus aureus, were utilized to assess the antibacterial and antifungal activities. Notably, the derivatives 1‐(phenyl (piperidine‐1‐yl) methyl)‐1H‐indole and 4‐(1H‐indol‐1‐yl) (phenyl) morpholine have strong antifungal effects on Candida albicans that are equivalent to those of the widely used drug fluconazole.

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Antibacterial activity of extracellular biosynthesized iron oxide nanoparticles against locally isolated β-lactamase-producing Escherichia coli from Egypt

Abou-Dobara,

Kamel,

El-Sayed

et al. 2024

Discov Appl Sci

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Nowadays, extended spectrum β-lactamase (ESBL) producing Escherichia coli has been recognized and recorded worldwide as one of the main causing agents and a major contributor to nosocomial infections. The current study aimed to isolate and detect β-lactamase-producing E. coli and use it in the extracellular biosynthesis of iron oxide nanoparticles (Fe2O3 NPs). Fifteen Gram-negative (G-ve), lactose-fermenting, negative citrate and non-spore-forming coliform bacteria were isolated from the total bacterial isolates from water samples. Different tests were performed to detect β-lactamase-producing E. coli isolates including the chromogenic methods as acidimetric and iodometric techniques and the phenotypic methods as cloverleaf test and Masuda double-disc test. ESBL-producing E. coli was detected and confirmed by a modified double disc synergy test using ceftazidime, cefotaxime, ceftriaxone, amoxicillin combined with clavulanic acid, imipenem, cefepime, and cefoxitin. The quantitative assay of β-lactamase was done using a micro-iodometric assay. Among E. coli bacterial isolates, S1B1 isolate (the highest isolate of β-lactamase activity) was selected and tested for the extracellular biosynthesis of Fe2O3 NPs. The produced nanoparticles (NPs) were characterized by UV–visible spectroscopy, X-ray diffraction analysis, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscope and Zeta analysis. Results confirmed the successful biosynthesis of Fe2O3 NPs which displayed an absorption peak at 346 nm and a Fe2O3 crystallographic lattice plane at (104). Fe2O3 NPs were negatively charged spherical-shaped NPs with an average size of ≈ 24 ± 2 nm. The FTIR spectrum refers to the presence of NPs-associated proteins which act as stabilizing and capping agents. Antibacterial activity of Fe2O3 NPs was tested against Staphylococcus aureus ATCC25923, Bacillus cereus ATCC6633 (G+ve bacterium), Pseudomonas aeruginosa ATCC27853 (G-ve bacterium), as well as the β-lactamase-producing E. coli S1B1 strain. Fe2O3 NPs revealed moderate to strong antibacterial action against the tested strains with a minimum inhibition concentration (MIC) ranging from 25 to 40 µg/ml.

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Biogenic Synthesis of Chitosan/Silver Nanocomposite by Escherichia coli D8 (MF062579) and its Antibacterial Activity

Cited by 3 publications

References 47 publications

Anticandidal applications of selenium nanoparticles biosynthesized with Limosilactobacillus fermentum (OR553490)

Anticandidal applications of selenium nanoparticles biosynthesized with Limosilactobacillus fermentum (OR553490)

Antimicrobial Activity of N‐Substituted Derivatives of 5‐Chloro‐2,3‐ Diphenyl Indole

Antibacterial activity of extracellular biosynthesized iron oxide nanoparticles against locally isolated β-lactamase-producing Escherichia coli from Egypt

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