Biogenic synthesis of silver nanoparticle from wasp nest soil fungus, Penicillium italicum and its analysis against multi drug resistance pathogens

Nayak, B. K.; Nanda, Anima; Prabhakar, V.

doi:10.1016/j.bcab.2018.09.014

Cited by 40 publications

(12 citation statements)

References 17 publications

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“…Most of the particles observed were spherical and separated from each other, with little agglomeration and size distribution between 10 and 30 nm. These results suggest that in this process, biological residues (capping agents) may have performed the function of reduction and stabilization of AgNPs 36,37 , as reported by Seetharaman et al 14 , Saravanan et al 19 and Balakumaran et al 38 in studies using different types of fungi. Analysis through Energy Dispersive X-ray (EDX) confirmed the presence of elemental silver signal (Fig.…”

Section: Effects Of Growth Time Of the Fungus Anamorphous Bjerkanderasupporting

confidence: 66%

Synthesis of silver nanoparticles using white-rot fungus Anamorphous Bjerkandera sp. R1: influence of silver nitrate concentration and fungus growth time

Osorio-Echavarría

Orozco

Gómez-Vanegas

2021

Sci Rep

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Currently, silver nanoparticles (AgNPs) constitute an interesting field of study in medicine, catalysis, optics, among others. For this reason, it has been necessary to develop new methodologies that allow a more efficient production of AgNPs with better antimicrobial and biological properties. In this research growth time effects Anamorphous Bjerkandera sp. R1 and the silver nitrate (AgNO3) concentration over AgNPs synthesis were studied. Through the protocol used in this work, it was found that the action of the capping proteins on the surface of the mycelium played a determining role in the reduction of the Ag+ ion to Ag0 nanoparticles producing a particle size that oscillated between 10 and 100 nm. The progress of the reaction was monitored using visible UV–Vis spectroscopy and the synthesized AgNPs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared radiation (FTIR) spectroscopy. The best synthetic properties were found at 1 mM of AgNO3 concentration, growth time of 8 days, and reaction time of 144 h. Nanometals obtention from microorganisms could be considered as a new method of synthesis, due to reducing abilities of metal ions through its enzymatic system and represents low-cost synthesis that reduces the generation of harmful toxic wastes.

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Section: Effects Of Growth Time Of the Fungus Anamorphous Bjerkanderasupporting

confidence: 66%

Synthesis of silver nanoparticles using white-rot fungus Anamorphous Bjerkandera sp. R1: influence of silver nitrate concentration and fungus growth time

Osorio-Echavarría

Orozco

Gómez-Vanegas

2021

Sci Rep

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“…A few others reported that the surface plasmon absorbance peaks range were about 412–440 nm ( Keller, 2004 ; Devika et al, 2012 ). The presence of plasmon band at 420 nm due to dipole plasmon resonance showing that the AgNPs have a spherical shape is reported by different researchers ( Nayak et al, 2018a ; Muthukrishnan et al, 2019a ). The particular mechanism for the biosynthesis of silver nanoparticles has not been clear yet, but it has been recommended that the fungal biomass contains reducing agents, such as NADH-dependent nitrate reductase enzyme, the nitrate reductase secreted by the fungal cells ( Devi and Joshi, 2012 ; Nanda et al, 2018 ).…”

Section: Resultsmentioning

confidence: 64%

“…Microbes are considered as important sources of bioactive natural products with enormous potential for the invention of new biomolecules for drug breakthrough, industrial utility, and agricultural applications ( Scott et al, 2008 ; Usha et al, 2010 ; Devaraj et al, 2013 ; Nayak et al, 2018a ). The earlier studies based on estimation of microbial populations have given away that only about 1% of bacteria and 5% of fungi are characterized and the rest remain uncharted for their contribution to human welfare ( Usha et al, 2010 ; Nayak et al, 2018a ). In recent years, the microbes have become resistant to the drugs and are subsequently modifying their genomic sequence.…”

Section: Introductionmentioning

confidence: 99%

“…Symbiotic microbes may represent a particularly promising source because microbial symbioses are widespread, widely unexplored for natural products ( Demain, 1999 ; Prakash and Thiagarajan, 2012 ) and often involve the exchange of small molecules between symbionts and host including compounds mediating host defense ( Sagar and Ashok, 2012 ; Nayak and Anitha, 2014 ; Bhat et al, 2015 ; Nayak et al, 2018b ). Among symbiotic associations, insect–microbe symbioses that involve fungi may be of particular interest in natural product discovery ( Sagar and Ashok, 2012 ; Nayak et al, 2018a ).…”

Section: Introductionmentioning

confidence: 99%

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Myco-Facilitated Biosynthesis of Nano-Silver From Wasp Nest Fungus, Paecilomyces variotii, and Its Antimicrobial Activity Against MTCC Strains

2022

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The utility of fungi as stabilizing and reducing agents in the biogenic synthesis of silver nanoparticles is striking due to the production of large quantities of biomolecules of minute toxic residuals. During the current study, sunlight- and dark-assessed silver nanoparticles were synthesized from wasp nest fungus, Paecilomyces variotii, at different pHs. Synthesized silver nanoparticles (AgNPs) at 6 pH were found to be more prominent than at 7 and 8 pHs. AgNPs were within the 20- to 90-nm range and were polygonal and elongated in shape. FTIR spectra of light-mediated AgNPs showed diverse transmittance bands than the silver nanoparticles synthesized in the dark. The synthesized AgNPs were found with diverse antimicrobial activities against pathogenic MTCC bacterial strains, i.e., Staphylococcus aureus, Vibrio parahaemolyticus, Escherichia coli, Shewanella putrefaciens, and fungus, Candida albicans. Aqueous filtrate and filtrate-mediated AgNPs combined with methanol solvent extract of yeast extract manitol broth (YEMB) had more inhibitory effects on all bacteria and Candida albicans. Furthermore, the combined effect of AgNPs and methanol solvent extract from YEMB culture filtrate was found more effective against E. coli, while AgNPs combined with methanol solvent of aqueous filtrate had inhibitory effects on E. coli and Candida albicans.

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“…In recent studies, many attempts have been made to improve the performance of CαAMPs. These strategies involve re-engineering natural CαAMP sequences by site-directed mutation (Ahmad et al, 2009;Irazazabal et al, 2016;Li et al, 2016;Jiang et al, 2020), fragmentation (Luo et al, 2021) or chemical modification (Zarina and Nanda, 2014;Nayak et al, 2018;Mourtada et al, 2019;Mwangi et al, 2019;Li et al, 2020), computational approaches based on natural templates and statistical analysis (Loose et al, 2006;Nagarajan et al, 2018Nagarajan et al, , 2019, or designing de novo sequences by using simple alkaline vs hydrophobic amino acid combinations (Deslouches et al, 2005(Deslouches et al, , 2013Hu et al, 2011;Lakshmaiah Narayana et al, 2020). However, partly due to the complexity of the structure-activity relationships, which are derived from the sequence diversity of the peptides, progress in CαAMP optimization is still very limited and few CαAMPs have successfully achieved FDA approval (Greber and Dawgul, 2017;Ghosh et al, 2019;Torres et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Fine-Tuning of Alkaline Residues on the Hydrophilic Face Provides a Non-toxic Cationic α-Helical Antimicrobial Peptide Against Antibiotic-Resistant ESKAPE Pathogens

Luo

Zhu

et al. 2021

Front. Microbiol.

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Antibiotic-resistant ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) has become a serious threat to public health worldwide. Cationic α-helical antimicrobial peptides (CαAMPs) have attracted much attention as promising solutions in post-antibiotic era. However, strong hemolytic activity and in vivo inefficacy have hindered their pharmaceutical development. Here, we attempt to address these obstacles by investigating BmKn2 and BmKn2-7, two scorpion-derived CαAMPs with the same hydrophobic face and a distinct hydrophilic face. Through structural comparison, mutant design and functional analyses, we found that while keeping the hydrophobic face unchanged, increasing the number of alkaline residues (i.e., Lys + Arg residues) on the hydrophilic face of BmKn2 reduces the hemolytic activity and broadens the antimicrobial spectrum. Strikingly, when keeping the total number of alkaline residues constant, increasing the number of Lys residues on the hydrophilic face of BmKn2-7 significantly reduces the hemolytic activity but does not influence the antimicrobial activity. BmKn2-7K, a mutant of BmKn2-7 in which all of the Arg residues on the hydrophilic face were replaced with Lys, showed the lowest hemolytic activity and potent antimicrobial activity against antibiotic-resistant ESKAPE pathogens. Moreover, in vivo experiments indicate that BmKn2-7K displays potent antimicrobial efficacy against both the penicillin-resistant S. aureus and the carbapenem- and multidrug-resistant A. baumannii, and is non-toxic at the antimicrobial dosages. Taken together, our work highlights the significant functional disparity of Lys vs Arg in the scorpion-derived antimicrobial peptide BmKn2-7, and provides a promising lead molecule for drug development against ESKAPE pathogens.

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Biogenic synthesis of silver nanoparticle from wasp nest soil fungus, Penicillium italicum and its analysis against multi drug resistance pathogens

Cited by 40 publications

References 17 publications

Synthesis of silver nanoparticles using white-rot fungus Anamorphous Bjerkandera sp. R1: influence of silver nitrate concentration and fungus growth time

Synthesis of silver nanoparticles using white-rot fungus Anamorphous Bjerkandera sp. R1: influence of silver nitrate concentration and fungus growth time

Myco-Facilitated Biosynthesis of Nano-Silver From Wasp Nest Fungus, Paecilomyces variotii, and Its Antimicrobial Activity Against MTCC Strains

Fine-Tuning of Alkaline Residues on the Hydrophilic Face Provides a Non-toxic Cationic α-Helical Antimicrobial Peptide Against Antibiotic-Resistant ESKAPE Pathogens

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