Biogenic zinc oxide nanoparticles-enhanced biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L

Abstract

Zinc oxide nanoparticles (NPs) have emerged as a novel elicitor for enhanced biosynthesis of secondary metabolites in in vitro plant cell cultures. The current study was aimed to explore elicitation abilities of ZnO-NPs for enhanced accumulation of lignans and neolignans in cell cultures of Linum usitatissimum. We optimized concentration of zinc oxide NPs before carrying out a full-fledged experiment. Subsequently, an optimum dose of 100 mg/l was introduced into the culture medium on day 0, days 0 and 15, and finally days 0 and 25. We observed that repeated elicitation stimulated various parameters and physiological responses in Linum usitatissimum cell cultures than one-time elicitation. Repeated elicitation of cell cultures on day 0 and 15 resulted in highest fresh weight (412.16 g/l) and lignans production (secoisolariciresinol diglucoside 284.12 mg/l: lariciresinol diglucoside 86.97 mg/l). Contrarily, repeated elicitation on day 0 and 25 resulted in highest DW (13.53 g/l), total phenolic production (537.44 mg/l), total flavonoid production (123.83 mg/l) and neolignans production (dehydrodiconiferyl alcohol glucoside 493.28 mg/l: guaiacylglycerol-b-coniferyl alcohol ether glucoside 307.69 mg/l). Enhancement in plant growth and secondary metabolites accumulation was several fold higher than controls. Furthermore, a linear relationship existed between total phenolic and flavonoid contents which in turn was correlated with higher antioxidant activities. ARTICLE HISTORY

Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis

“…Accordingly, the concentration of Zn provided to plants should be precisely controlled for optimal plant development [ 24 ]. On the contrary, zinc oxide nanoparticles (ZnONPs) elicitation capability has been used to promote the production of bioactive secondary metabolites in medicinal plants [ 25 , 26 ].…”

<p>Nanoparticles Affect the Expression Stability of Housekeeping Genes in Plant Cells</p>

“…Magnetic NPs negatively affect the metabolism and induce biosynthesis of protective compounds in suspension cultures of Tobacco BY-2 cells. 22 Biomass fresh weight and production of secondary metabolites reportedly increase after treatment with zinc oxide 23 and silver 24 NPs in cell suspension cultures of Linum usitatissimum. Copper oxide NPs treatment significantly increases the production of gymnemic acid II and phenolic compounds in Gymnema sylvestre suspension cell cultures.…”

Nano-Elicitation as an Effective and Emerging Strategy for In Vitro Production of Industrially Important Flavonoids

“…Seventy-five milligrams per liter of biogenic ZnONPs was reported to produce 3.7 mg/g DW flavonoids as compared to commercially available micro-sized ZnO, which produced 3.01 mg/g DW flavonoids in callus culture of Echinacea purpurea [67]. Biogenic ZnONPs effectively elicited in vitro flavonoid production in a cell suspension culture of Flax [68]. The shoot culture of S. rebaudiana and Lilium ledebourii showed increased secondary metabolism and TFC in response to engineered ZnONP elicitation [69,70].…”