Biogeochemical activity of microbial biofilms in the water column overlying uranium mine tailings

Bondici, Viorica F.; Khan, Nurul H.; Swerhone, G. D. W.; Dynes, James J.; Lawrence, John R.; Yergeau, Étienne; Wolfaardt, Gideon; Warner, Jeffrey A.; Korber, Darren R.

doi:10.1111/jam.12593

Cited by 16 publications

(4 citation statements)

References 66 publications

(183 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, we found a high relative abundance of Aquabacterium ( Figure 4 , ASV 17033, Betaproteobacteriales, Gammaproteobacteria) in the entire water column of the western subtropical Pacific Ocean. Bondici et al (2014) found the nature of Aquabacterium detected in the water column and the presence of reduced iron suggested that complex hydrocarbons were available for their growth and geochemical change, such as iron reduction. These observations raise questions on their adaptation to the marine environments they inhabit and the ecological functions they probably perform.…”

Section: Discussionmentioning

confidence: 99%

Biodiversity and Biogeography of Abundant and Rare Microbial Assemblages in the Western Subtropical Pacific Ocean

et al. 2022

View full text Add to dashboard Cite

The levels of chlorophyll a and nutrient concentrations in the surface waters of the western subtropical Pacific Ocean are among the lowest globally. In addition, our knowledge of basin-scale diversity and biogeography of microbial communities in this vast extremely oligotrophic environment is still rather limited. Here, high-throughput sequencing was used to examine the biodiversity and biogeography of abundant and rare microbial assemblages throughout the water column from the surface to a depth of 3,000 m across a horizontal distance of 1,100 km in the western Pacific Ocean. Microbial alpha diversity in the 200-m layer was higher than at other depths, with Gammaproteobacteria, Alphaproteobacteria, and Clostridia as the dominant classes in all samples. Distinctly vertical distributions within the microbial communities were revealed, with no difference horizontally. Some microbes exhibited depth stratification. For example, the relative abundances of Cyanobacteria and Alphaproteobacteria decreased with depth, while Nitrososphaeria, Actinobacteria, and Gammaproteobacteria increased with depth in the aphotic layers. Furthermore, we found that environmental (selective process) and spatial (neutral process) factors had different effects on abundant and rare taxa. Geographical distance showed little effect on the dispersal of all and abundant taxa, while statistically significant distance–decay relationships were observed among the rare taxa. Temperature and chlorophyll a were strongly associated with all, abundant, and rare taxa in the photic layers, while total inorganic nitrogen was recognized as the crucial factor in the aphotic layers. Variance partitioning analysis indicated that environmental selection played a relatively important role in shaping all and abundant taxa, while the variation in rare taxa explained by environmental and spatial processes was relatively low, as more than 70% of the variation remained unexplained. This study provides novel knowledge related to microbial community diversity in the western subtropical Pacific Ocean, and the analyzes biogeographical patterns among abundant and rare taxa.

show abstract

Section: Discussionmentioning

confidence: 99%

Biodiversity and Biogeography of Abundant and Rare Microbial Assemblages in the Western Subtropical Pacific Ocean

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Most studies of the microbial response to AgNPs have been conducted with planktonic cells, ,− even though bacteria in the environment commonly attach to surfaces and form biofilms . Whereas biofilms can beneficially participate in biogeochemical cycles, waste degradation, and clean energy generation, − they are also implicated in biofouling, biocorrosion, pathogenic infection, and disease outbreaks. − Thus, prevention of biofilm formation is one objective of incorporating AgNPs on material surfaces. ,, …”

Section: Introductionmentioning

confidence: 99%

Sublethal Concentrations of Silver Nanoparticles Stimulate Biofilm Development

Yang

Alvarez

2015

Environ. Sci. Technol. Lett.

View full text Add to dashboard Cite

Although silver nanoparticles (AgNPs) are used as antimicrobial agents in a wide variety of commercial products, sublethal exposure can counterproductively promote the development of biofilms. We observed by fluorescence microscopy denser biofilm growth with mixed cultures from a wastewater treatment plant after exposure to 21.6 μg/L 10 nm AgNPs. To further understand biofilm promotion mechanisms, experiments were conducted with a pure culture of Pseudomonas aeruginosa PAO1. Sublethal exposure of PAO1 to AgNPs (10.8 and 21.6 μg/L) also enhanced biofilm development and upregulated quorum sensing, lipopolysaccharide biosynthesis, and antibiotic resistance (efflux pump) genes. An increase in the sugar and protein contents of the PAO1 biofilm matrix (by 55 ± 3 and 114 ± 32%, respectively, relative to unexposed controls) corroborated the transcriptional upregulation of PAO1 biofilm-related genes. Enhanced biofilm development by exposure to low AgNP concentrations might accelerate biofouling and biocorrosion and harbor pathogens that pose a risk to public health.

show abstract

“…On the other hand, the relatively significant genus in the inoculated sludge was Candidatus , while the dominant micro‐organisms in sample FS2 changed into the denitrificating bacteria of genera Hydrogenophaga and Dechloromonas , due to enrichment cultivation (Achenbach et al, 2001; Bondici et al, 2014). Besides, the relative abundances of the genera Thauera , Azospirillum and Hyphomicrobium in the biofilm significantly increased as compared with those in the inoculated sludge, all of which are closely related to denitrification function (Chowdhury et al, 2007; Pacovsky, 1990; Zhang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Synergistic denitrification and phosphorus removal performance of a biofilm-microflocculation system and its microbial community variations: A pilot-scale study for a wastewater treatment plant

Jiang

Yang

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: For upgrading and reconstructing a municipal wastewater treatment plant, a biofilm-microflocculation filter system was designed and established towards synergistic improvement of denitrification and phosphorus removal from the secondary effluent. Methods and Results:The establishment of the biofilm-microflocculation filter system underwent several processes, including sludge inoculation, biofilm formation and polyaluminum chloride (PAC) addition as flocculating agent. Microbial community analysis indicated that the dominant denitrification bacteria of the biofilm filter were in the phylum Proteobacteria and the genera Hydrogenophaga and Dechloromonas. On the basis of the initiation of filter system under optimal parameters such as C/N ratio of 5.3, hydraulic retention time of 1.06 h and PAC of 5 mg L −1 , approximately 75% COD, 80% TN and 75% TP could be effectively removed to satisfy discharge standards. Comparing the variations of microbial community structure at the genus level during the operating period of the filter system, it was found that the relative abundance of denitrification bacteria merely shifted from 53.14% to 48.76%, demonstrating that the effect of PAC addition on the main micro-organisms is marginal. Conclusions:From the above results, it can be verified that the established biofilmmicroflocculation filter system has practical and reliable performance for simultaneous biological denitrification and phosphorus removal.

show abstract

Biogeochemical activity of microbial biofilms in the water column overlying uranium mine tailings

Cited by 16 publications

References 66 publications

Biodiversity and Biogeography of Abundant and Rare Microbial Assemblages in the Western Subtropical Pacific Ocean

Biodiversity and Biogeography of Abundant and Rare Microbial Assemblages in the Western Subtropical Pacific Ocean

Sublethal Concentrations of Silver Nanoparticles Stimulate Biofilm Development

Synergistic denitrification and phosphorus removal performance of a biofilm-microflocculation system and its microbial community variations: A pilot-scale study for a wastewater treatment plant

Contact Info

Product

Resources

About