Biogeographic variation in Mytilus galloprovincialis heat shock gene expression across the eastern Pacific range

Dutton, Jessica M.; Hofmann, Gretchen E.

doi:10.1016/j.jembe.2009.06.001

Cited by 34 publications

(20 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this scenario, the 40ЊC heat treatment would have exceeded the induction temperature in northern lizards but not in southern ones. Geographic variation in Hsp70 induction temperature has been previously shown in intertidal mollusks (Tomanek and Somero 1999;Dutton and Hofmann 2009), insects (Dahlhoff and Rank 2000), amphibians ), and reptiles (Ulmasov et al 1992). With minimal regional effects on air temperature in our study, the difference in the Hsp70 response between northern and southern collections of S. occidentalis can possibly be explained by long-lasting developmental effects or local adaptation.…”

Section: Discussionsupporting

confidence: 65%

Geographic Variation in the Effects of Heat Exposure on Maximum Sprint Speed and Hsp70 Expression in the Western Fence LizardSceloporus occidentalis

McMillan

Irschick²,

Rees³

2011

Physiological and Biochemical Zoology

View full text Add to dashboard Cite

We examined whether western fence lizards Sceloporus occidentalis occurring in thermally divergent environments display differential responses to high temperature in locomotor performance and heat-shock protein (Hsp) expression. We measured maximum sprint speed in S. occidentalis from four populations at paired latitudes and elevations before and after exposure to an experimental heat treatment and then quantified hind-limb muscle Hsp70 expression. Lizards collected from northern or high-elevation collection sites suffered a greater reduction in sprint speed after heat exposure than lizards collected from southern or low-elevation sites. In addition, lizards from northern collection sites also exhibited an increase in Hsp70 expression after heat exposure, whereas there was no effect of heat exposure on Hsp70 expression in lizards from southern collection sites. Across all groups, there was a negative relationship between Hsp70 expression and sprint speed after thermal stress. This result is significant because (a) it suggests that an increase in Hsp70 alone cannot compensate for the immediate negative effects of high-temperature exposure on sprint speed and (b) it demonstrates a novel correlation between an emergent property at the intersection of several physiological systems (locomotion) and a cellular response (Hsp70 expression). Ultimately, geographic variation in the effects of heat on sprint speed may translate into differential fitness and population viability during future increases in global air temperatures.

show abstract

Section: Discussionsupporting

confidence: 65%

Geographic Variation in the Effects of Heat Exposure on Maximum Sprint Speed and Hsp70 Expression in the Western Fence LizardSceloporus occidentalis

McMillan

Irschick²,

Rees³

2011

Physiological and Biochemical Zoology

View full text Add to dashboard Cite

show abstract

“…Changes in gene expression play an important role in physiological resilience to thermal stress (Hofmann 2005;Teranishi & Stillman 2007;Gracey et al 2008;Place et al 2008;Dutton & Hofmann 2009;Lockwood et al 2010). Recent evidence in intertidal limpets, mussels and periwinkle snails suggests that concentration increases of molecular chaperones such as heat shock proteins (Hsps) can repair heat-induced cellular damage (Bedulina et al 2010;Dong et al 2010;Lesser et al 2010;Wang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

RNA‐seq reveals regional differences in transcriptome response to heat stress in the marine snail Chlorostoma funebralis

2015

View full text Add to dashboard Cite

To investigate the role of gene expression in adaptation of marine ectotherms to different temperatures, we examined the transcriptome-wide thermal stress response in geographically separated populations of the intertidal snail Chlorostoma funebralis. Snails from two southern (heat tolerant) and two northern (heat sensitive) populations were acclimated to a common thermal environment, exposed to an environmentally relevant thermal stress and analysed using RNA-seq. Pooling across all populations revealed 306 genes with differential expression between control and heat-stressed samples, including 163 significantly upregulated and 143 significantly downregulated genes. When considered separately, regional differences in response were widely apparent. Heat shock proteins (Hsps) were upregulated in both regions, but the magnitude of response was significantly greater in northern populations for most Hsp70s, while the southern populations showed greater upregulation for approximately half of the Hsp40s. Of 177 stress-responsive genes in northern populations, 55 responded to heat stress only in northern populations. Several molecular chaperones and antioxidant genes that were not differentially expressed in southern populations showed higher expression under control conditions compared with northern populations. This suggests that evolution of elevated expression of these genes under benign conditions preadapts the southern populations to frequent heat stress and contributes to their higher thermal tolerance. These results indicate that evolution has resulted in different transcriptome responses across populations, including upregulation of genes in response to stress and preadaptation of genes in anticipation of stress (based on evolutionary history of frequent heat exposure). The relative importance of the two mechanisms differs among gene families and among populations.

show abstract

“…Previous studies on thermal tolerance (e.g. bay scallops, Argopecten irradians, Brun, Bricelj, MacRae, & Ross, 2008; and Mediterranean mussels, Mytilus galloprovincialis, Dutton & Hofmann, 2009) demonstrate that more frequent exposure to temperature extremes results in elevated heat shock proteins (HSPs) and HSP mRNA transcripts. In addition, Sørensen, Kristensen, and Loeschcke (2003) found that many species exhibit heritable HSP production patterns.…”

Section: Mortalitymentioning

confidence: 99%

Evidence of Ostrea lurida Carpenter, 1864, population structure in Puget Sound, WA, USA

et al. 2017

View full text Add to dashboard Cite

Species traits that carry adaptive advantages such as reproductive timing and stress resilience may differ among populations. Knowledge and consideration of these traits should, therefore, be integrated into conservation efforts that include long‐term persistence of species. To test for adaptive differences between Olympia oyster, Ostrea lurida, populations a reciprocal transplant experiment was carried out monitoring survival, growth and reproduction using three established populations of O. lurida within Puget Sound, WA, USA. Performance differed for each population. Ostrea lurida from Dabob Bay had the highest survival at all sites but the lowest reproductive activity and growth. Oysters from Oyster Bay demonstrated the greatest proportion of brooding females at a majority of sites with moderate growth and survival. Together these data suggest the existence of O. lurida population structure within Puget Sound and provide information on how broodstock should be selected for restoration purposes.

show abstract

Biogeographic variation in Mytilus galloprovincialis heat shock gene expression across the eastern Pacific range

Cited by 34 publications

References 29 publications

Geographic Variation in the Effects of Heat Exposure on Maximum Sprint Speed and Hsp70 Expression in the Western Fence LizardSceloporus occidentalis

Geographic Variation in the Effects of Heat Exposure on Maximum Sprint Speed and Hsp70 Expression in the Western Fence LizardSceloporus occidentalis

RNA‐seq reveals regional differences in transcriptome response to heat stress in the marine snail Chlorostoma funebralis

Evidence of Ostrea lurida Carpenter, 1864, population structure in Puget Sound, WA, USA

Contact Info

Product

Resources

About