Biogeography, autecology, and phylogeny of Percolomonads based on newly described species

Hohlfeld, Manon; Meyer, Claudia; Schoenle, Alexandra; Nitsche, Frank; Arndt, Hartmut

doi:10.1111/jeu.12930

Cited by 5 publications

(5 citation statements)

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“…Our findings support their cosmopolitan distribution and show that they are also widely distributed in benthic habitats around the Azores islands at various depths. The species Lula levis and Nonamonas santamariensis were not found in two major metabarcoding datasets in our previous study [ 63 ], leading to the assumption that they are restricted in their distribution. However, the current dataset suggests that they are as well widely distributed around the Azores islands in a wide range of depths.…”

Section: Resultsmentioning

confidence: 98%

“…The samples analyzed in this study were also used for a cultivation-based approach [ 15 ], resulting in several monoclonal cultures belonging mainly to Cafeteriaceae [ 50 ] and Percolomonadida [ 63 ]. We used these groups as model groups to check whether we can find V9 sequences belonging to our cultivated strains in the metabarcoding dataset.…”

Section: Resultsmentioning

confidence: 99%

“…The high proportion of rediscovered sequences of cultivated strains supports the reliability of the dataset analyzed in this study. Both Cafeteria burkhardae and Percolomonas adaptabilis were previously described to be widely distributed in marine habitats all over the world [ 49 , 63 ]. Our findings support their cosmopolitan distribution and show that they are also widely distributed in benthic habitats around the Azores islands at various depths.…”

Section: Resultsmentioning

confidence: 99%

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Distribution Patterns of Benthic Protist Communities Depending on Depth Revealed by Environmental Sequencing—From the Sublittoral to the Deep Sea

Hohlfeld

Arndt

2023

Microorganisms

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Protists are key components of the microbial food web in marine pelagic systems because they link algal and bacterial production to higher trophic levels. However, their functioning and bathymetric distribution in benthic deep-sea ecosystems are still only poorly understood. However, biogeographical patterns of communities can be coupled to the functioning of ecosystems and are therefore important to understand ecological and evolutionary processes. In this study, we investigated the diversity and distribution of benthic protist communities from the sublittoral down to the deep seafloor (50–2000 m) around three islands of the Azores in the North Atlantic Ocean. Using amplicon sequencing of the V9 region (18S rDNA) of 21 samples, we found that protist community compositions from different depths were significantly different. Three assemblages were separated along the following depths: 50 m, 150–500 m and 1000–2000 m, which indicate that deep-sea areas surrounding islands might act as isolating barriers for benthic protist species. A limited gene flow between the communities could favor speciation processes, leading to the unique protist communities found at the different investigated islands.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Distribution Patterns of Benthic Protist Communities Depending on Depth Revealed by Environmental Sequencing—From the Sublittoral to the Deep Sea

Hohlfeld

Arndt

2023

Microorganisms

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“…from Azores) each form an individual branch. This may be signs for an allopatric speciation in remote areas/islands(Hernandez-Hernandez et al, 2021) as it was postulated for percolomonads and other protist species(Arndt et al, 2020;Hohlfeld et al, 2022). Jet, genetic differences between different protist genera can widely vary depending on the genus and family.…”

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confidence: 88%

Cryptic Cryptophytes – revision of the genusGoniomonas

Sachs,

Nitsche,

Arndt

2024

Preprint

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Cryptomonad protists are ubiquitously distributed over marine and freshwater habitats. As an exception to the coloured cryptomonads, the heterotrophic cryptomonads of the genusGoniomonashave an ancestral phylogenetic position. They lack any kind of chloroplast and most likely represent a basal group to those cryptomonad groups having obtained their chloroplast by secondary endosymbiosis. Earlier studies have shown a deep divergence between freshwater and marine clades of goniomonads that comprise large genetic distances between members within the group and also between the two groups of marine and freshwater taxa. Still, marine and freshwater species carry the same genus name, and until up to date only few species have been described. We therefore restructured goniomonad systematics based not only on a separation of marine and freshwater taxa, but, taking the large genetic distances into account, also several new genera are described. Based on morphological as well as phylogenetic data (18S rDNA sequences), this leads to the formation of the freshwater generaLimnogoniomonasn. g.,GoniomonasandAquagoniomonasn. g. and the marine generaNeptunogoniomonasn. g.,Marigoniomonasn. g.,Thalassogoniomonasn. g.,Poseidogoniomonasn. g. andCosmogoniomonasn. g. To give the restructuring process a stable basis, we additionally propose a neotype forGoniomonas truncata.

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“…), in saline microbial mats and hypersaline lagoons harboring biosignatures classified as diatoms, bicosoecids, and ciliates as detected by metabarcoding (Saghaï et al, 2017;Rybarski et al, 2023). Efforts to isolate and sequence the cultivable and uncultivable protist diversity from saline to hypersaline lagoons unveiled a novel endemic diversity including species from Opisthokonta (choanoflagellates), Rhizaria (Cercomonadida), Discoba (percolomonads), Stramenopiles (bicosoecids and placidids), and Alveolata (ciliates) (Schiwitza et al, 2018(Schiwitza et al, , 2019(Schiwitza et al, , 2021Arndt et al, 2020;Carduck et al, 2021;Rybarski et al, 2021Rybarski et al, , 2023Schoenle et al, 2022;Acosta et al, 2023;Hohlfeld et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Protist communities of microbial mats from the extreme environments of five saline Andean lagoons at high altitudes in the Atacama Desert

Acosta,

Nitsche,

Dorador

et al. 2024

Front. Microbiol.

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IntroductionHeterotrophic protists colonizing microbial mats have received little attention over the last few years, despite their importance in microbial food webs. A significant challenge originates from the fact that many protists remain uncultivable and their functions remain poorly understood.MethodsMetabarcoding studies of protists in microbial mats across high-altitude lagoons of different salinities (4.3–34 practical salinity units) were carried out to provide insights into their vertical stratification at the millimeter scale. DNA and cDNA were analyzed for selected stations.ResultsSequence variants classified as the amoeboid rhizarian Rhogostoma and the ciliate Euplotes were found to be common members of the heterotrophic protist communities. They were accompanied by diatoms and kinetoplastids. Correlation analyses point to the salinity of the water column as a main driver influencing the structure of the protist communities at the five studied microbial mats. The active part of the protist communities was detected to be higher at lower salinities (<20 practical salinity units).DiscussionWe found a restricted overlap of the protist community between the different microbial mats indicating the uniqueness of these different aquatic habitats. On the other hand, the dominating genotypes present in metabarcoding were similar and could be isolated and sequenced in comparative studies (Rhogostoma, Euplotes, Neobodo). Our results provide a snapshot of the unculturable protist diversity thriving the benthic zone of five athalossohaline lagoons across the Andean plateau.

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Biogeography, autecology, and phylogeny of Percolomonads based on newly described species

Cited by 5 publications

References 39 publications

Distribution Patterns of Benthic Protist Communities Depending on Depth Revealed by Environmental Sequencing—From the Sublittoral to the Deep Sea

Distribution Patterns of Benthic Protist Communities Depending on Depth Revealed by Environmental Sequencing—From the Sublittoral to the Deep Sea

Cryptic Cryptophytes – revision of the genusGoniomonas

Protist communities of microbial mats from the extreme environments of five saline Andean lagoons at high altitudes in the Atacama Desert

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