Biography of Joshua R. Sanes

Davis, Thomas J.

doi:10.1073/pnas.0408739102

Cited by 8 publications

(14 citation statements)

References 23 publications

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“…The authors proposed that the Pcdh-γs that are present in perisynaptic astrocytic processes promote synaptogenesis, which is in agreement with our observation with the localization of Pcdh-γC5. Nevertheless, synaptogenesis occurs in the absence of astrocytes (as we have shown above) and in the total absence of Pcdh-γs expression in the mouse Pcdh-γ KOs (Wang et al, 2002; Weiner et al, 2005). Moreover, we have found that Pcdh-γC5 clusters that are present at contact points between neurons and astrocytes, are not necessarily perisynaptic, suggesting a role for the Pcdh-γC5-mediated neuronal-astrocyte interactions wider than promoting neuronal connectivity.…”

Section: Discussionmentioning

confidence: 60%

“…These mutants also showed apoptosis of interneurons in the spinal cord (see also Prasad et al, 2008), which has been interpreted as Pcdh-γs having a role in neuronal survival, thus complicating the interpretation of the results in terms of a possible synaptic role of the Pcdh-γs. Weiner et al (2005) generated a Pcdh-γ KO mutant that also lacked the proapoptotic protein Bax. These double KO animals had normal neuronal survival but reduced number of glutamatergic and GABAergic synapses and smaller excitatory and inhibitory synaptic currents in the spinal cord.…”

Section: Discussionmentioning

confidence: 99%

“…An antibody that recognizes all Pcdh-γs (Phillips et al, 2003) and several mouse knock out (KO) mutants, in which the whole Pcdh-γ family has been deleted (Wang et al, 2002; Hambsch et al, 2005; Weiner et al, 2005; Lefebvre et al, 2008; Garret and Weiner, 2009), have been used for studying the distribution and function of the combined Pcdh-γ family. Specific hybridization probes have also been used to study the brain distribution and developmental expression of the mRNAs of the individual members of the Pcdh-γ family by in situ hybridization and Northern blot analysis (Frank et al, 2005; Zou et al, 2007; Allen Brain Atlas http://www.brain-map.org).…”

Section: Introductionmentioning

confidence: 99%

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Synaptic and nonsynaptic localization of protocadherin‐γC5 in the rat brain

Serwanski

Miralles

et al. 2010

J of Comparative Neurology

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It has been proposed that γ-protocadherins (Pcdh-γs) are involved in the establishment of specific patterns of neuronal connectivity. Contrary to the other Pcdh-γs, which are expressed in the embryo, Pcdh-γC5 is expressed postnatally in the brain, coinciding with the peak of synaptogenesis. We have developed an antibody specific for Pcdh-γC5 to study the expression and localization of Pcdh-γC5 in brain. Pcdh-γC5 is highly expressed in the olfactory bulb, corpus striatum, dentate gyrus, CA1 region of the hippocampus, layers I and II of the cerebral cortex, the molecular layer of the cerebellum. Pcdh-γC5 is expressed in both neurons and astrocytes. In hippocampal neuronal cultures, and in the absence of astrocytes, a significant percentage of synapses, more GABAergic than glutamatergic, have associated Pcdh-γC5 clusters. Some GABAergic axons show Pcdh-γC5 in the majority of their synapses. Nevertheless, many Pcdh-γC5 clusters are not associated with synapses. In the brain, a significant number of Pcdh-γC5 clusters are located at contact points between neurons and astrocytes. Electron microscope immunocytochemistry of the rat brain shows that i) Pcdh-γC5 is present in some GABAergic and glutamatergic synapses both pre- and postsynaptically; ii) Pcdh-γC5 is also extrasynaptically localized in membranes and in cytoplasmic organelles of neurons and astrocytes; and iii) that Pcdh-γC5 is also localized in perisynaptic astrocyte processes. The results support the notion that i) Pcdh-γC5 plays a role in synaptic specificity and/or synaptic maturation, and ii) that Pcdh-γC5 is involved in neuron-neuron synaptic interactions and in neuron-astrocyte interactions, including perisynaptic neuron-astrocyte interactions.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synaptic and nonsynaptic localization of protocadherin‐γC5 in the rat brain

Serwanski

Miralles

et al. 2010

J of Comparative Neurology

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“…Datasets NKI (http://ccb.nki.nl/data/) and GSE3494 (http://www.ncbi.nlm.nih.gov/geo/) were used for validation [31, 32]. Robust Multichip Average (RMA) [51] method was used for normalization.…”

Section: Methodsmentioning

confidence: 99%

Gene regulatory pattern analysis reveals essential role of core transcriptional factors’ activation in triple-negative breast cancer

Min

Zhang

et al. 2017

Oncotarget

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BackgroundTriple-negative breast cancer (TNBC) is an aggressive breast cancer subtype. Genome-scale molecular characteristics and regulatory mechanisms that distinguish TNBC from other subtypes remain incompletely characterized.ResultsBy combining gene expression analysis and PANDA network, we defined three different TF regulatory patterns. A core TNBC-Specific TF Activation Driven Pattern (TNBCac) was specifically identified in TNBC by computational analysis. The essentialness of core TFs (ZEB1, MZF1, SOX10) in TNBC was highlighted and validated by cell proliferation analysis. Furthermore, 13 out of 35 co-targeted genes were also validated to be targeted by ZEB1, MZF1 and SOX10 in TNBC cell lines by real-time quantitative PCR. In three breast cancer cohorts, non-TNBC patients could be stratified into two subgroups by the 35 co-targeted genes along with 5 TFs, and the subgroup that more resembled TNBC had a worse prognosis.MethodsWe constructed gene regulatory networks in breast cancer by Passing Attributes between Networks for Data Assimilation (PANDA). Co-regulatory modules were specifically identified in TNBC by computational analysis, while the essentialness of core translational factors (TF) in TNBC was highlighted and validated by in vitro experiments. Prognostic effects of different factors were measured by Log-rank test and displayed by Kaplan-Meier plots.ConclusionsWe identified a core co-regulatory module specifically existing in TNBC, which enabled subtype re-classification and provided a biologically feasible view of breast cancer.

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“…gambiae and anti-fungal responses in Ae. aegypti 113; 114 . The intracellular signaling module of the Toll and Imd pathways in mosquitoes is very similar to that in Drosophila , although genes involved in pathogen detection and effector molecules show some diversification, which is expected due to different selection pressures 112 .…”

Section: Nf-κb Innate Immune Pathwaysmentioning

confidence: 99%

Insect Antiviral Innate Immunity: Pathways, Effectors, and Connections

Kingsolver

Huang

Hardy

2013

Journal of Molecular Biology

274

271

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Insects are infected by a wide array of viruses some of which are insect-restricted and pathogenic, and some of which are transmitted by biting insects to vertebrates. The medical and economic importance of these viruses heightens the need to understand the interaction between the infecting pathogen and the insect immune system in order to develop transmission interventions. The interaction of the virus with the insect host innate immune system plays a critical role in the outcome of infection. The major mechanism of antiviral defense is the siRNA pathway that responds through the detection of virus-derived dsRNA to suppress virus replication. However, other innate antimicrobial pathways such as Imd, Toll, Jak-STAT, and the autophagy pathway have also been shown to play important roles in antiviral immunity. In this review we provide an overview of the current understanding of the main insect antiviral pathways and examine recent findings that further our understanding of the roles of these pathways in facilitating a systemic and specific response to infecting viruses.

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Biography of Joshua R. Sanes

Cited by 8 publications

References 23 publications

Synaptic and nonsynaptic localization of protocadherin‐γC5 in the rat brain

Synaptic and nonsynaptic localization of protocadherin‐γC5 in the rat brain

Gene regulatory pattern analysis reveals essential role of core transcriptional factors’ activation in triple-negative breast cancer

Insect Antiviral Innate Immunity: Pathways, Effectors, and Connections

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