BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS

Abstract

Background: Bioactive compounds from the fruit of S. elaeagnifolium were isolated since could be highly potential source to develop functional foods or pharmaceutical products. Objectives: In this study a bioguided fractionation of the methanolic extract from S. elaeagnifolium fruit was carried out to evaluate their cytotoxicity and antitumoral potential on several cell lines and breast tumor explants, respectively. Methods: Microdilution method with A. salina was used to isolate bioactive compounds. Fractionation was performed by vacuum liquid chromatography, and the monitoring from fractions was done by thin layer chromatography. The effect of the fractions on viability in Vero, HeLa, and MCF-7 cells was assessed using WST-1 assay, whereas in breast tumor explants was evaluated by Alamar blue assay. A qualitative phytochemical analysis was performed to partially identify the compounds contained in the fractions and a spectroscopic characterization by RP-HPLC-MS was done to identify the group of compounds responsible for the effect on the cell lines and the mammary explants. Results: Several fractions were isolated from the fruit of S. elaeagnifolim. Notwithstanding, the FVLC7 showed a higher activity in A. salina assay. This fraction reduced the viability at 39 ± 1.67, 15.05 ± 0.09 and 66.10 ± 4 % in Vero, HeLa and MCF-7 cells, at 100 mg/mL, respectively. On the other hand, showed an effect in breast tumor explants obtained from a patient in remission. Qualitative phytochemical analysis showed that FVLC7 contains alkaloids, coumarins, and sesquiterpene lactones. Characterization by RP-HPLC-MS detected quinic acid, chlorogenic acid, dicaffeoylquinic acid as well as presence of an alkaloid. Conclusion: On this basis, our results suggest that cytotoxic effect of FVLC7 isolated from the fruit of S. elaeagnifolium could be mediated by quinic, chlorogenic, and dicaffeoylquinic acids.

Deciphering the effects of kosmotrope and chaotrope salts during aqueous two phase extraction (ATPE) of polyphenolic compounds and glycoalkaloids from the leaves of a nutraceutical plant, Solanum retroflexum, with the aid of UPLC-QTOF-MS

“…2. This highlighted that caffeoyl malate was synthesized from the esterification reaction of caffeic acid and malic acid in a similar way to which Solanum plants would synthesize CQA from CA and QA [14,40,41]. It is worth noting that caffeoyl malate was not detected in any other Solanum species to date, though similar metabolites such as feuralic malate and coumaric malate have been reported in Fabaceae plants, P. vulgaris, [27].…”

CARACTERIZACIÓN, DIGESTIBILIDAD Y EFECTO PREBIOTICO DEL EXTRACTO DEL FRUTO DE Stenocereus queretaroensis F.A.C. WEBER BUXBAUM

“…The difference in pH could be due to the concentration of chemical constituents (Harris, 2016). Furthermore, the pH can vary depending on the phenological conditions of the plant and the maturity of the fruit because its composition is related to the conditions of harvesting, post-harvest storage, biosynthesis, geographical region, season and age of the plant (Hernández et al, 2017).…”

The Ultrasound-Assisted Extraction of Polyphenols from Mexican Firecracker (Hamelia patens Jacq.): Evaluation of Bioactivities and Identification of Phytochemicals by HPLC-ESI-MS

“…According to the database ReSpect for Phytochemicals [29] and Hernández et al [28], the parent ion with m/z 395 may correspond to an alkaloid (peak 9). However, the information could not be corroborated since no fragments were reported for the MS 2 analysis; for that reason, it was considered an unknown compound.…”