Biohydrogen and 5-aminolevulinic acid production from waste barley by Rhodobacter sphaeroides O.U.001 in a biorefinery concept

Cumhuriyet Science Journal

Ceylan

2019

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In the present study, valorization of waste barley by producing hydrogen (H2) and 5-aminolevulinic acid (5-ALA) using Rhodobacter sphaeroides O.U.001 was aimed. Firstly, 3 % (w/v) waste barley hydrolysate was prepared by treating 3 g of powdered waste barley with H2SO4 in a total volume of 100 mL mixture and then autoclaving this mixture at 121 ºC for 30 min. Upon generation of fermentable simple sugars by pretreatment and analytical examination of the hydrolysate in terms of ammonium content, element composition and light transmittance, various types of growth media containing various concentrations of sugar (5-6-7-8 g/L) were prepared. The cells were cultivated in these media under photo-heterotrophic conditions which favor H2 and 5-ALA generations. pH changes, growth, H2 production and 5-ALA generation were monitored in the media. The results showed that all the media prepared from 3 % (w/v) waste barley hydrolysate sustained the cell growth appreciably. The highest OD value (OD660: 1.71) was attained when using 8 g/L sugar. Furthermore, biological H2 evolution was seen in each bioreactor. In particular, the highest hydrogen accumulation (0.29 L H2/L) was achieved in 6 g/L sugar-containing medium. However, 5-ALA was not detected in any of the media. To conclude, considerable cell growth and biological hydrogen production was achieved using 3 % (w/v) waste barley hydrolysate under the conditions tested but there was no detectable 5-ALA generation.

Section: Ph Changes and Bacterial Growthsupporting

confidence: 69%

Ön işlemden geçirilmiş atık arpa kullanarak Rhodobacter sphaeroides O.U.001 ile hidrojen üretimi

Cumhuriyet Science Journal

Ceylan

2019

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“…(11). Aminolevulinate (Aminolevulinic acid, 5-ALA) is metabolized to PpIX through heme pathway (12). PpIX activation induces the generation of singlet oxygen species in mitochondria and cell death is experienced (13,14,15).…”

Section: Introductionmentioning

confidence: 99%

Revealing the therapeutic effects of aminolevulinate mediated femtosecond laser induced photo-chemotherapy in different cancer cells

The EuroBiotech Journal

Yıldırım

Gündoğdu

et al. 2020

Photodynamic therapy (PDT) is a photo chemotherapeutic strategy that is the application of photosensitizing agent and light on disease or tumor site. The aim of this study is to confirm the feasibility for femtosecond (fs) laser for aminolevulinate (ALA) mediated PDT on skin, breast and bladder cancer cells. Also the remarkable aspects of ALA mediated and laser induced PDT with respect to other literally known applications were investigated.Metastatic melanoma cells SK-MEL30, mammary epithelial carcinoma cells MCF-7 and bladder cancer cells UMUC-3 were treated with ALA and then the cells were irradiated by fs laser at thirty wavelengths in between 230 and 800 nm for 30s and 60s. Anti-cancer effects of ALA phototherapy on different cancer cell lines were determined. Protoporphyrin IX (PpIX) accumulation was visualized by confocal microscopy. The effective PDT wavelengths were applied to evaluate the degree of apoptosis and necrosis in cells.The viability tests demonstrated that wavelengths 400-440 nm and 600-630 nm were found to decrease the viability on three model cell lines. PDT at 630 nm exerted cell death by necrosis and apoptosis after 30 s and 60 s periods.This paper confirms that ALA and femtosecond laser mediated PDT may be used together as therapeutic and diagnostic method to target breast, skin and urinary bladder cancer cells. The use of fs laser allows the flexibility for optimization of wavelength for photosensitizing agents.

“…Rhodobacter sphaeroides is a robust microorganism which can generate several high value-added products like hydrogen [1,2,3], 5-aminolevulinic acid (5-ALA, 5-amino-4-oxo-pentanoic acid) [1,2] and poly-β-hydroxybutyric acid [4]. As being non-pathogenic and non-immunogenic [5], R. sphaeroides could be a safe and sustainable platform for the green generation of high value-added products.…”

Section: Introductionmentioning

confidence: 99%

“…(1) 2H + + 2e -+ 4ATP → H2 + 4ADP + 4Pi (2) Recently, there is an increasing concern in the world on the use of hydrogen as a biofuel in transportation vehicles like cars. This will in turn promote the research on hydrogen production technologies.…”

Section: Introductionmentioning

confidence: 99%

“…One of the pillars of the biological hydrogen production process is the substrate since the total reducing power of the cell is mainly determined by the type of substrate and its assimilation pathway in the cell. For instance, organic acids like lactate, malate and acetate and simple sugars like glucose and fructose could readily be used by PNS bacteria for hydrogen productions [2,12,14]. Among them, acetate is a two-carbon compound and its theoretical conversion into hydrogen is given in Equation 3.…”

Section: Introductionmentioning

confidence: 99%

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Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001

International Journal of Energy Applications and Technologies

Alparslan

2020

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The objective of this work was to reveal optimum culture composition for hydrogen and 5aminolevulinic acid productions by Rhodobacter sphaeroides O.U.001 regarding substrate concentration and supplementations of elements and vitamins. Acetate was chosen as carbon source and five distinct concentrations (20, 25, 30, 35 and 40 mM) were tested in two experimental setups. While, elements (FeSO4, 2 g L-1 and Na2MoO4.2H2O, 0.2 g L-1) and vitamins (Biotin, 0.015 g L-1 , Niacin, 0.5 g L-1 and Thiamine, 0.5 g L-1) were added into the media in the first setup, they were omitted in the latter for comparison. As a result, the highest hydrogen production (0.33 L H2 L-1 culture) was attained in the presence of supplements using 20 mM acetate. Similarly, the maximum amount of 5-ALA generation (16.54 mM) was achieved in 20 mM acetate containing medium under the same conditions. On the other hand, the greatest bacterial growth (OD660: 4.412, 2.162 g cdw L-1) was achieved in the absence of supplements using 40 mM acetate. To conclude, while element and vitamin supplementations promoted hydrogen and 5-ALA productions, absence of these had a positive effect on cell biomass. Specifically, the medium containing 20 mM acetate together with elements and vitamins could be suggested as the optimum growth culture for the highest hydrogen and 5-ALA productions.