Biohydrogen production by dark fermentation: Experiences of continuous operation in large lab scale

Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant

“…A crucial point for a biotechnological approach is that of devising methods to optimise and stabilise the microbial consortia that ensure high productivity [2,3], as well as elucidating the biochemical pathways that can be tuned or controlled for the hydrogen production purpose. A particular interest concerning the precise mode and timing of hydrogen production is relevant for bio-hytane production, a very promising fuel that can be obtained in two-phase dark fermentation plants by suitably combining bio-hydrogen and bio-methane streams [4].…”

Biorefineries – factories of the future

“…Dark fermentation differs from photofermentation because it only occurs in the absence of light (Levin et al, 2004). Dark fermentation (Krupp and Widmann, 2009) refers to the production of H 2 by various heterotrophic bacteria that may be cultured in a variety of optimal conditions. Unfortunately, in practice not only H 2 but also other gases, mainly CO 2 , CH 4 and volatile fatty acids, are produced depending on the route used for fermentation.…”

Feasibility of Bioenergy Production from Ultrafiltration Whey Permeate Using the UASB Reactors

“…It is well known that only biological hydrogen production processes from the fermentation of renewable substrates, such as organic wastewater or other wastes are the promising alternative for hydrogen generation. Several strategies for the production of biohydrogen by fermentation in lab-scale have been found in the literature: photo-fermentation (Gadhamshetty et al, 2008), dark-fermentation (Krupp & Widmann, 2009) and combined-fermentation, which refers to the two fermentations combined (Nath & Das, 2009). However, no strategies for industrial scale productions have been found.…”