Biohydrogen production from fermentation of cotton stalk hydrolysate by Klebsiella sp. WL1316 newly isolated from wild carp (Cyprinus carpio L.) of the Tarim River basin

Li, Yanbin; Zhang, Qin; Deng, Li; Liu, Zhanwen; Jiang, Hui; Fang, Wang

doi:10.1007/s00253-018-8882-z

Cited by 32 publications

(25 citation statements)

References 40 publications

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“…The initial conditions (neutral pH) are usually more suitable for methane production (Rabii et al, 2019) than hydrogen production. However, dark fermentation for some substrates in nonacidic pH can give high volumes of hydrogen like Li et al (2018) Klebsiella sp. WL1316, could effectively produce a high yield of hydrogen by using cotton stalk hydrolysate as substrate.…”

Section: Resultsmentioning

confidence: 99%

Hydrogen and Methane Production Under Conditions of Anaerobic Digestion of Key-Lime and Cabbage Wastes

et al. 2019

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In this article, the results of key lime fruit (Citrus aurantifolia) wastes and cabbage (Brassica L.) wastes anaerobic digestion are presented. Anaerobic digestion of the wastes was performed in batch process, neutral pH (key-lime 7.47 and cabbage 7.67) and substrate concentration of Volatile Suspended Solids (VSS) 10 gVSS/L. One of the aims of this research was to check the availability of these substrates to be the source of methane and hydrogen. Key lime wastes produced 32 times more methane than raw cabbage. However, hydrogen production from cabbage was 149 times higher than key lime. The percentage of methane production in cabbage was up to 81% and in key lime was up to 75%. This research showed from the substrates comparison that efficient hydrogen production is less dependent on low pKa, pH than on total solids of the substrates.

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Section: Resultsmentioning

confidence: 99%

Hydrogen and Methane Production Under Conditions of Anaerobic Digestion of Key-Lime and Cabbage Wastes

et al. 2019

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“…The fermentable sugar solution of the cotton stalk hydrolysate was prepared following optimum hydrolysis, then detoxified and decolorized as previously described [37,38,45]. The main sugar components in the hydrolysate solution were glucose and xylose [38,46], which were modified to a previously determined optimum concentration (total reducing sugar concentration was 40 g/L) and used as the substrate medium in the following fermentation experiments.…”

Section: Preparation Of Cotton Stalk Hydrolysatementioning

confidence: 99%

Enhanced biohydrogen production from cotton stalk hydrolysate of Enterobacter cloacae WL1318 by overexpression of the formate hydrogen lyase activator gene

Zhang

You

et al. 2020

Biotechnol Biofuels

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Background: Biohydrogen production from lignocellulose has become an important hydrogen production method due to its diversity, renewability, and cheapness. Overexpression of the formate hydrogen lyase activator (fhlA) gene is a promising tactic for enhancement of hydrogen production in facultative anaerobic Enterobacter. As a species of Enterobacter, Enterobacter cloacae was reported as a highly efficient hydrogen-producing bacterium. However, little work has been reported in terms of cloning and expressing the fhlA gene in E. cloacae for lignocellulose-based hydrogen production. Results: In this study, the formate hydrogen lyase activator (fhlA) gene was cloned and overexpressed in Enterobacter cloacae WL1318. We found that the recombinant strain significantly enhanced cumulative hydrogen production by 188% following fermentation of cotton stalk hydrolysate for 24 h, and maintained improved production above 30% throughout the fermentation process compared to the wild strain. Accordingly, overexpression of the fhlA gene resulted in an enhanced hydrogen production potential (P) and maximum hydrogen production rate (R m), as well as a shortened lag phase time (λ) for the recombinant strain. Additionally, the recombinant strain also displayed improved glucose (12%) and xylose (3.4%) consumption and hydrogen yield Y(H 2 /S) (37.0%) compared to the wild strain. Moreover, the metabolites and specific enzyme profiles demonstrated that reduced flux in the competitive branch, including succinic, acetic, and lactic acids, and ethanol generation, coupled with increased flux in the pyruvate node and formate splitting branch, benefited hydrogen synthesis. Conclusions: The results conclusively prove that overexpression of fhlA gene in E. cloacae WL1318 can effectively enhance the hydrogen production from cotton stalk hydrolysate, and reduce the metabolic flux in the competitive branch. It is the first attempt to engineer the fhlA gene in the hydrogen-producing bacterium E. cloacae. This work provides a highly efficient engineered bacterium for biohydrogen production from fermentation of lignocellulosic hydrolysate in the future.

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“…An interesting issue, undertaken in this research, is related to the possibility of using cotton waste, including cotton stalk [18] as a source of hydrogen. Fast depletion of fossil fuels [19] means that there is a growing need to seek renewable ways of hydrogen production, an important resource for chemistry [20] and carbon free fuel [8].…”

Section: Introductionmentioning

confidence: 99%

“…In most cases the optimal dark fermentation process proceeds under acidic conditions (pH ~ 5.0), at mesophilic temperature and using stressed inoculum. However, for some substrates like potato waste [31] or cotton stalk, optimal conditions [18] are closer to pH neutral or basic. Therefore, it is important to clarify these differences and determine conditions (including pH, temperature, microaeration rate, etc.)…”

Section: Introductionmentioning

confidence: 99%

Rapid hydrogen generation from cotton wastes by mean of dark fermentation

et al. 2020

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Dark fermentation of textile wastes is discussed in the paper. In the experiment cotton wastes were fermented. Before fermentation the cotton was hydrolyzed using 0.1 M HCl acidic solution. The inoculum was pretreated by means of heat shock for 0.5 h at 105 °C. The fermentation was carried out under mesophilic conditions at a load of 5 g VSS/L, and pH 5. Oxygen was added in small quantities during fermentation. The oxygen flow rates (OFR) were between 0.3 and 1.0 mL/h. The fermentation was carried out for a few days at temperatures between 40 and 43 °C. Hydrogenesis prevailed at the lower temperature (40 °C) and methanogenesis at the higher (43 °C). Conversion of cotton waste to methane (3.4%) was slightly higher than conversion to hydrogen (2.6%). The highest hydrogen production was obtained for OFR 0.8 mL/h and the percentage of hydrogen in biogas was 43%. At higher temperatures (43 °C) no hydrogen production was observed

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Biohydrogen production from fermentation of cotton stalk hydrolysate by Klebsiella sp. WL1316 newly isolated from wild carp (Cyprinus carpio L.) of the Tarim River basin

Cited by 32 publications

References 40 publications

Hydrogen and Methane Production Under Conditions of Anaerobic Digestion of Key-Lime and Cabbage Wastes

Hydrogen and Methane Production Under Conditions of Anaerobic Digestion of Key-Lime and Cabbage Wastes

Enhanced biohydrogen production from cotton stalk hydrolysate of Enterobacter cloacae WL1318 by overexpression of the formate hydrogen lyase activator gene

Rapid hydrogen generation from cotton wastes by mean of dark fermentation

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