Biohydrogen production from waste materials: benefits and challenges

Kamaraj, M.; Ramachandran, K. K.; Aravind, J.

doi:10.1007/s13762-019-02577-z

Cited by 66 publications

(17 citation statements)

References 149 publications

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“…Introducing the calculated solutions into Equations (5) and 9, we obtain the finalized equations: (14) Reactions (13) and (14) allow the estimation of methane and hydrogen recovery from an arbitrary substrate with the formula C a H b O c N d S e P f Me g , based on the following equations:…”

Section: Yield Estimationmentioning

confidence: 99%

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Sulfur, Phosphorus and Metals in the Stoichiometric Estimation of Biomethane and Biohydrogen Yields

2020

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The estimation of biomethane or biohydrogen yield is used to evaluate energy recovery during the process of the anaerobic treatment of waste and wastewater. Mathematically calculated theoretical values can also be used in biomethane or biohydrogen potential tests as reference points to calculate which fraction of substrate is decomposed, when the substrate degradation stopped and when the sample’s self-digestion begins. This study suggests expanded forms of equations for anaerobic processes leading to either biomethane or biohydrogen. The traditional equations describing the conversion of a substrate with known carbon, hydrogen, oxygen and nitrogen composition were expanded to account for the composition of sulfur (for biohydrogen yields) and phosphorus (both biohydrogen and biomethane yields). As an optional part, one metal cation was also incorporated into the chemical formula of the evaluated wastewater composition in case the compound of biodegradable interest exists as a salt. The equations derived here can be useful for researchers estimating energy recovery based on the elemental analysis of samples, such as algal biomass harvested during harmful algal blooms (HABs). Examples of biomethane and biohydrogen yield estimations from sulfur- and phosphorus-containing compounds are also provided.

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Section: Yield Estimationmentioning

confidence: 99%

“…Biomethane and biohydrogen are two main products considered in energy recovery from waste and wastewater [1][2][3][4], which are considered to be a replacement for conventional fossil fuels [5][6][7][8]. The anaerobic digestion process leading to those products is described by equations [9][10][11]:…”

Section: Introductionmentioning

confidence: 99%

Sulfur, Phosphorus and Metals in the Stoichiometric Estimation of Biomethane and Biohydrogen Yields

2020

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“…Hydrogen is the cleanest energy on earth, and has become the most competitively exploited renewable form of energy in the world [6][7][8][9]. In recent years, biohydrogen production from lignocellulose has become an important hydrogen production method due to its diversity, renewability, and cheapness [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Enhanced biohydrogen production from cotton stalk hydrolysate of Enterobacter cloacae WL1318 by overexpression of the formate hydrogen lyase activator gene

Zhang

You

et al. 2020

Preprint

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Background Biohydrogen production from lignocellulose has become an important hydrogen production method due to its diversity, renewability, and cheapness. Overexpression of the formate hydrogen lyase activator ( fhlA ) gene is a promising tactic for enhancement of hydrogen production in facultative anaerobic Enterobacter . However, little work has been reported in terms of the fhlA gene cloning and expression in Enterobacter cloacae as well as the engineered strain for lignocellulose-based hydrogen production.Results The formate hydrogen lyase activator ( fhlA ) gene was cloned and overexpressed in Enterobacter cloacae WL1318, and the cumulative hydrogen production and dynamics, glucose and xylose consumption, cell growth, and soluble metabolites were analyzed in the wild and recombinant strains. The results showed that the recombinant strain significantly enhanced cumulative hydrogen production by 188% following fermentation of cotton stalk hydrolysate for 24 h, and maintained improved production above 30% throughout the fermentation process compared to the wild strain. Accordingly, overexpression of the fhlA gene resulted in an enhanced hydrogen production potential ( P ) and maximum hydrogen production rate ( R m ), as well as a shortened lag phase time ( λ ) for the recombinant strain. Additionally, the recombinant strain also displayed improved glucose (12%) and xylose (3.4%) consumption and hydrogen yield Y(H 2 /S) (37.0%) compared to the wild strain. Moreover, the metabolites and specific enzyme profiles demonstrated that reduced flux in the competitive branch, including succinic, acetic, and lactic acids, and ethanol generation, coupled with increased flux in the pyruvate node and formate splitting branch, benefited hydrogen synthesis.Conclusions The results conclusively prove that overexpression of fhlA gene in E. cloacae WL1318 can effectively enhance the hydrogen production from cotton stalk hydrolysate, and reduce the metabolic flux in the competitive branch. It’s the first attempt to engineer the fhlA gene in the hydrogen producing bacterium E. cloacae . This work provides a highly efficient engineered bacterium for biohydrogen production from fermentation of lignocellulosic hydrolysate in the future.

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“…With the worsening energy crisis and exhaustion of traditional fossil energy dominated by petroleum, energy production based on lignocellulose bioconversion has attracted worldwide attention [1][2][3][4][5]. Hydrogen is the cleanest energy on earth, and has become the most competitively exploited renewable form of energy in the world [6][7][8][9]. In recent years, biohydrogen production from lignocellulose has become an important hydrogen production method due to its diversity, renewability, and cheapness [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Enhanced biohydrogen production from cotton stalk hydrolysate of Enterobacter cloacae WL1318 by overexpression of the formate hydrogen lyase activator gene

Zhang

You

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Biohydrogen production from lignocellulose has become an important hydrogen production method due to its diversity, renewability, and cheapness. Overexpression of the formate hydrogen lyase activator (fhlA) gene is a promising tactic for enhancement of hydrogen production in facultative anaerobic Enterobacter. As a species of Enterobacter, Enterobacter cloacae was reported as high efficient hydrogen-producing bacterium. However, little work has been reported in terms of cloning and expressing the fhlA gene in E. cloacae for lignocellulose-based hydrogen production.Results: In this study, the formate hydrogen lyase activator (fhlA) gene was cloned and overexpressed in Enterobacter cloacae WL1318. We found that the recombinant strain significantly enhanced cumulative hydrogen production by 188% following fermentation of cotton stalk hydrolysate for 24 h, and maintained improved production above 30% throughout the fermentation process compared to the wild strain. Accordingly, overexpression of the fhlA gene resulted in an enhanced hydrogen production potential (P) and maximum hydrogen production rate (Rm), as well as a shortened lag phase time (λ) for the recombinant strain. Additionally, the recombinant strain also displayed improved glucose (12%) and xylose (3.4%) consumption and hydrogen yield Y(H2/S) (37.0%) compared to the wild strain. Moreover, the metabolites and specific enzyme profiles demonstrated that reduced flux in the competitive branch, including succinic, acetic, and lactic acids, and ethanol generation, coupled with increased flux in the pyruvate node and formate splitting branch, benefited hydrogen synthesis. Conclusions: The results conclusively prove that overexpression of fhlA gene in E. cloacae WL1318 can effectively enhance the hydrogen production from cotton stalk hydrolysate, and reduce the metabolic flux in the competitive branch. It’s the first attempt to engineer the fhlA gene in the hydrogen producing bacterium E. cloacae. This work provides a highly efficient engineered bacterium for biohydrogen production from fermentation of lignocellulosic hydrolysate in the future.

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Biohydrogen production from waste materials: benefits and challenges

Cited by 66 publications

References 149 publications

Sulfur, Phosphorus and Metals in the Stoichiometric Estimation of Biomethane and Biohydrogen Yields

Sulfur, Phosphorus and Metals in the Stoichiometric Estimation of Biomethane and Biohydrogen Yields

Enhanced biohydrogen production from cotton stalk hydrolysate of Enterobacter cloacae WL1318 by overexpression of the formate hydrogen lyase activator gene

Enhanced biohydrogen production from cotton stalk hydrolysate of Enterobacter cloacae WL1318 by overexpression of the formate hydrogen lyase activator gene

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