Biohydrogenation of Linoleic Acid by Anaerobic Bacteria Isolated from Rumen

Fujimoto, Kenshiro; Kimoto, Hiromi; Shishikura, Mieko; Endo, Yasushi; Ogimoto, Keiji

doi:10.1271/bbb.57.1026

Cited by 25 publications

(14 citation statements)

References 2 publications

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“…Of the 10 10 -10 11 bacteria residing in the rumen of a dairy cow, only a few species are thought to carry out these biohydrogenation reactions which may function as a means of protection against the toxic effects of the PUFAs to the producing organisms (Lock & Bauman, 2004). One of the best known ruminal microorganisms capable of CLA production is the strict anaerobe Butyrivibrio fibrisolvens (Fujimoto, Kimoto, Shishikura, Endo, & Ogimoto, 1993), a microorganism also found in human faeces (Brown & Moore, 1960). Indeed, a strain of B. fibrisolvens has recently been touted as a potential probiotic treatment for animals to provide a constant supply of CLA (Fukuda, Suzuki, Murai, Asanuma, & Hino, 2006).…”

Section: Microbial Production Of Clamentioning

confidence: 99%

Specific metabolite production by gut microbiota as a basis for probiotic function

Ross

Mills

Hill

et al. 2010

International Dairy Journal

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Section: Microbial Production Of Clamentioning

confidence: 99%

Specific metabolite production by gut microbiota as a basis for probiotic function

Ross

Mills

Hill

et al. 2010

International Dairy Journal

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“…The trans C18:1 FA is an intermediate produced during ruminal biohydrogenation of C18:3 and C18:2; many isomers of the trans C18:1 FA have been identified, e.g., trans-9 C18:1 (Fujimoto et al 1993), trans-15, and trans-11 C18:1 (Harfoot and Hazlewood 1988). The trans-11 C18:1 is the most abundant trans isomer of C18:1 produced in the rumen under normal feeding conditions (Harfoot and Hazlewood 1988).…”

Section: Growth Stage and N Fertilization (Experiments 1)mentioning

confidence: 99%

Fatty acids in forages. II. In vitro ruminal biohydrogenation of linolenic and linoleic acids from timothy

Boufaïed¹,

Chouinard²,

Tremblay³

et al. 2003

Can. J. Anim. Sci.

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G. 2003. Fatty acids in forages. II. In vitro ruminal biohydrogenation of linolenic and linoleic acids from timothy. Can. J. Anim Sci. 83: 513-522. Polyunsaturated fatty acids from forages are hydrogenated in the rumen and this biohydrogenation depends on several factors. The objective of this study was to determine and compare in vitro ruminal biohydrogenation of linolenic (C18:3) and linoleic (C18:2) acids in timothy harvested from different growth stages, produced with or without fertilization, or conserved by different methods. In exp. 1, timothy was grown with (120 kg ha -1 ) and without N and harvested at four growth stages. In exp. 2, harvested timothy was conserved as fresh grass, wilted grass, dry hay, haylage, and silage, some with additives: haylage with lactic acid bateria (LAB), haylage with formic acid, silage with LAB, and silage with formic acid. Samples were incubated with rumen fluid in three trials to compare: (1) samples from exp. 1; (2) the five basic conserving methods of exp. 2, and; (3) how the silage additives affected biohydrogenation of C18:3 and C18:2. Effective disappearances and ruminal bypass of C18:3 and C18:2 were fastest in timothy harvested at stem elongation but slowed linearly with increasing maturity (P < 0.01). Although N fertilization increased (P < 0.01) the effective disappearance and bypass of C18:3 and the bypass of C18:2, it did not affect their rate of disappearance. These last rates were not affected by maturity, but were faster in haylage and silage than in hay (P < 0.01). Ruminal bypass of C18:3 was greater in fresh grass, wilted grass, and dry hay than in haylage and silage (P < 0.01). Additions of formic acid and LAB to haylage and silage had no significant effect on C18:3 and C18:2 disappearances and bypass. To maximize the amount of C18:3 that bypasses ruminal biohydrogenation timothy should be fertilized with N, harvested at an early growth stage, and conserved as dry hay. (1) les échantillons de l'expérience 1; (2) les cinq méthodes de conservation de base de l'expérience 2 et; (3) comment est-ce que les additifs pour ensilage affectent la biohydrogénation des C18:3 et C18:2. L'hydrogénation effective et le bypass ruminal des C18:3 et C18:2 étaient élevés dans la fléole récoltée au début montaison, puis diminuaient linéairement avec la maturité (P < 0,01). La fertilisation azotée augmentait (P < 0,01) l'hydrogénation effective et le bypass des C18:3 ainsi que le bypass des C18:2, mais n'affectait pas les taux de biohydrogénation des C18:3 et C18:2. Ces derniers taux n'étaient pas affectés par la maturité, mais ils étaient plus élevés pour l'ensilage préfané et l'ensilage humide que pour le foin (P < 0,01). Le bypass ruminal des C18:3 était plus élevé dans le fourrage frais, le fourrage préfané et le foin que dans l'ensilage préfané et l'ensilage humide (P < 0,01). L'addition d'acide formique ou de LAB à l'ensilage préfané et l'ensilage humide de fléole n'avait pas d'effet sur l'hydrogénation et le bypass ruminal des C18:3 et C18:2. La quantité de C18:3 qui ...

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“…The chemical structure of CLA1 was not identiˆed: it may be cis-9,trans-11-or trans-9,cis-11-18:2. 5-7) Some studies found that linoleic acid is converted to 9,11-18:2 by several species of rumen bacteria [8][9][10][11][12][13][14][15] and by dairy starter cultures. 16) However, to our knowledge, exact identiˆcation of the geometric conˆguration of the 9,11-18:2 produced has not been done.…”

mentioning

confidence: 99%

Structural Analysis of Conjugated Linoleic Acid Produced byLactobacillus plantarum, and Factors Affecting Isomer Production

Kishino

Ogawa

Ando

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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Biohydrogenation of Linoleic Acid by Anaerobic Bacteria Isolated from Rumen

Cited by 25 publications

References 2 publications

Specific metabolite production by gut microbiota as a basis for probiotic function

Specific metabolite production by gut microbiota as a basis for probiotic function

Fatty acids in forages. II. In vitro ruminal biohydrogenation of linolenic and linoleic acids from timothy

Structural Analysis of Conjugated Linoleic Acid Produced byLactobacillus plantarum, and Factors Affecting Isomer Production

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