Bioindicators from Mosquitofish (Gambusia affinis) Sampled from the Imperial Valley in Southern California

Jenkins, Jill A.; Draugelis-Dale, Rassa O.

doi:10.3133/ofr20061307

Cited by 8 publications

(14 citation statements)

References 52 publications

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“…Genotoxic effects can increase the number of diploid spermatids due to a failure of meitotic chromosomes to segregate (Hacker-Klom et al, 1986). These LSS spermatogenic staging results were similar to those seen with western mosquitofish (Gambusia affinis) in an environment polluted with insecticides and organochlorines, at which the ploidy values showed fewer mature sperm forms at contaminated sites (Jenkins and Draugelis-Dale, 2006;Jenkins et al, 2009).…”

Section: Sperm Quality Parameterssupporting

confidence: 70%

“…Data were generated by flow cytometry, collecting 10K events in duplicate per sample. Gating was used to exclude doublets, and the percentage of haploid nuclei out of the total haploid plus over-haploid events was determined (Jenkins, 2011b;Jenkins and Draugelis-Dale, 2006). …”

Section: Spermatogenic Stagingmentioning

confidence: 99%

“…Based on cessation of motility, heat inactivation for LSS spermatozoa (2 × 10 8 cells/ml) occurred at 68°C for 10 min. Using both dead and live spermatozoa, viability and mitochondrial membrane potential (MMP) assays were then validated (using 318 mOsm/kg buffer) (Jenkins and Draugelis-Dale, 2006).…”

Section: Validation Assays and Antibiotics Selectionmentioning

confidence: 99%

“…Likewise, viability and MMP were 90.7% (SE 0.03) and 90.8% (SE 0.03) respectively in the 494 mOsm/kg buffer, but 97.0% (SE 0.01) and 97.0% (SE 0.01) respectively in the 318 mOsm/kg buffer. Validation assays (n = 3 fish) for viability and MMP using shipped milt or sperm from shipped testes (Jenkins and Draugelis-Dale, 2006) generated R 2 values ranging from 0.9476 to 0.9929. Therefore the 320 mOsm/kg HBSS was selected for shipment and testes were chosen over milt because extracted milt from shipped testes yielded better sperm quality.…”

Section: Shipping Buffersmentioning

confidence: 99%

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Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Jenkins

Olivier

Draugelis-Dale

et al. 2014

Science of The Total Environment

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Section: Sperm Quality Parameterssupporting

confidence: 70%

Section: Spermatogenic Stagingmentioning

confidence: 99%

Section: Validation Assays and Antibiotics Selectionmentioning

confidence: 99%

Section: Shipping Buffersmentioning

confidence: 99%

See 2 more Smart Citations

Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Jenkins

Olivier

Draugelis-Dale

et al. 2014

Science of The Total Environment

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“…Testicular cell counts were generated based on tissue mass after cell resuspension and filtering through 30 μm nylon mesh (Small Parts, Miami Lakes, FL) (Jenkins and Draugelis-Dale, 2006). In 2009, milt was fixed in 4% paraformaldehyde and sperm counts were based on volume of milt.…”

Section: Testicular Cell Countsmentioning

confidence: 99%

Reproductive health of yellow perch Perca flavescens in selected tributaries of the Chesapeake Bay

Blazer

Pinkney

Jenkins

et al. 2013

Science of The Total Environment

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Iwanowicz, Luke R.; Minkkinen, Steven; Draugelis-Dale, Rassa O.; and Uphoff, James H., "Reproductive health of yellow perch Perca flavescens in selected tributaries of the Chesapeake Bay" (2013 H I G H L I G H T S► Reduced recruitment of yellow perch has occurred in urban tributaries of Chesapeake Bay. ► We compared reproductive health biomarkers in perch from two urban, one developing, two less developed watersheds. ► Lack of final maturation, abnormal yolk and zona pellucida were noted in females. ► Leydig cell proliferation was noted in males. Reduced recruitment of yellow perch has been noted for a number of years in certain urbanized watersheds (South and Severn Rivers) of the Chesapeake Bay. Other rapidly developing watersheds such as Mattawoman Creek are more recently showing evidence of reduced recruitment of anadromous fishes. In this study, we used a battery of biomarkers to better document the reproductive health of adult yellow perch collected during spring spawning in [2007][2008][2009]. Perch were collected in the South and Severn Rivers, Mattawoman Creek and the less developed Choptank and Allen's Fresh watersheds for comparison. Gonadosomatic indices, plasma reproductive hormone concentrations, plasma vitellogenin concentrations and gonad histology were evaluated in mature perch of both sexes. In addition, sperm quantity (cell counts) and quality (total and progressive motility, spermatogenic stage and DNA integrity), were measured in male perch. Many of these biomarkers varied annually and spatially, with some interesting statistical results and trends. Male perch from the Choptank and Allen's Fresh had generally higher sperm counts. In 2008 counts were significantly lower in the perch from the Severn when compared to other sites. The major microscopic gonadal abnormality in males was the proliferation of putative Leydig cells, observed in testes from Severn and less commonly, Mattawoman Creek perch. Observations that could significantly impact egg viability were an apparent lack of final maturation, abnormal yolk and thin, irregular zona pellucida. These were observed primarily in ovaries from Severn, South and less commonly Mattawoman Creek perch. The potential association of these observations with urbanization, impervious surface and chemical contaminants is discussed. a b s t r a c t a r t i c l e i n f oPublished by Elsevier B.V.

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Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)

Jenkins

Draugelis-Dale

Pinkney³

et al. 2015

Theriogenology

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Bioindicators from Mosquitofish (Gambusia affinis) Sampled from the Imperial Valley in Southern California

Cited by 8 publications

References 52 publications

Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Reproductive health of yellow perch Perca flavescens in selected tributaries of the Chesapeake Bay

Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)

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