Bioinert Surface of Pluronic-Immobilized Flask for Preservation of Hematopoietic Stem Cells

Higuchi, Akon; Aoki, Nobuo; Yamamoto, Taro; Gomei, Yumiko; Egashira, Satsuki; Matsuoka, Yuki; Miyazaki, Toshihisa; Fukushima, Hisashi; Jyujyoji, Shin; Natori, Shizue

doi:10.1021/bm050868n

Cited by 19 publications

(19 citation statements)

References 27 publications

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“…Therefore, most cells do not grow on these polymers, which have been used as tissue adhesion barriers. However, it was shown that Pluronics® can be a good substrate for hematopoietic stem cells, supporting their culture and preservation more than conventional tissue culture dishes [57,58]. Recently, the use of Pluronic® F127 (synonymous to poloxamer 407) was reported for tissue engineering applications.…”

Section: Peo/ppo-based Systemsmentioning

confidence: 99%

Thermoresponsive hydrogels in biomedical applications

Klouda

Mikos

2008

European Journal of Pharmaceutics and Biopharmaceutics

1,135

696

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Environmentally responsive hydrogels have the ability to turn from solution to gel when a specific stimulus is applied. Thermoresponsive hydrogels utilize temperature change as the trigger that determines their gelling behavior without any additional external factor. These hydrogels have been interesting for biomedical uses as they can swell in situ under physiological conditions and provide the advantage of convenient administration. The scope of this paper is to review the aqueous polymer solutions that exhibit transition to gel upon temperature change. Typically, aqueous solutions of hydrogels used in biomedical applications are liquid at ambient temperature and gel at physiological temperature. The review focuses mainly on hydrogels based on natural polymers, N-isopropylacrylamide polymers, poly(ethylene oxide)-b-poly(propylene oxide)-bpoly(ethylene oxide) polymers as well as poly(ethylene glycol)-biodegradable polyester copolymers.

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Section: Peo/ppo-based Systemsmentioning

confidence: 99%

Thermoresponsive hydrogels in biomedical applications

Klouda

Mikos

2008

European Journal of Pharmaceutics and Biopharmaceutics

1,135

696

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“…Also, Pluronic F127 was activated using carbonyldiimidazole and immobilized on the surface of a lysine-coated polystyrene tissue culture flask. 23 In this study, new materials with improved characteristics are prepared by mixing PVA and Pluronic F127 in aqueous solutions and their rheological properties are investigated in order to design thermoresponsive materials with potential biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Temperature Responsive Gels Based on Pluronic F127 and Poly(vinyl alcohol)

Bercea¹,

Darie²,

Niţă³

et al. 2011

Ind. Eng. Chem. Res.

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Aqueous mixtures of Pluronic F127 and poly(vinyl alcohol) (20% polymer concentration in the system) were prepared and investigated by rheology and laser particle size distribution, in order to obtain temperature responsive gels for biomedical applications. The influence of the gelation conditions and system composition on the viscoelastic parameters and particle size distribution (which are very sensitive to the sol-gel transition process) was followed. The investigations realized at physiological temperature (37°C) showed the thixotropic profile of the gels, the yield stress induced by the Pluronic presence, and the high elasticity evidenced in the creep-recovery tests.

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“…albumin, or fibrinogen) on the PEO‐PPO‐PEO surface and did not involve comparison of different single proteins or examine mixed proteins in a complex media. Therefore, although the low biofouling property of PEO‐PPO‐PEO triblock copolymers has been demonstrated previously,22, 24, 25 there is still a lack of complete study of protein adsorption from both single protein solutions and complex media using well‐controlled PEO‐PPO‐PEO surfaces 16, 26…”

Section: Introductionmentioning

confidence: 99%

A systematic SPR study of human plasma protein adsorption behavior on the controlled surface packing of self‐assembled poly(ethylene oxide) triblock copolymer surfaces

Chang

Chu

Chen

et al. 2009

J Biomedical Materials Res

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A well-controlled biocompatible nonfouling surface is significant for biomedical requirements, especially for the improvement of biocompatibility. We demonstrate the low or nonbiofouling surfaces by coating hydrophobic-hydrophilic triblock copolymers of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) on the CH(3)-terminated self-assembled monolayer (SAM). Two types of copolymers are used to modify the surface, one with different PEO/PPO ratios ( approximately 20/80, 40/60, and 80/20, w/w) but the same PPO molecular weight ( approximately 2 k), the other with different copolymer MWs ( approximately 9, 11, and 15 k) but the same PEO/PPO ratio (80/20, w/w). In situ surface plasmon resonance (SPR) sensor is used to evaluate polymer adsorption on the SAMs and subsequent protein adsorption on the copolymer-treated surface. The effects of PEO-PPO-PEO molecular weight, PPO-to-PEO ratio, and ionic strength on protein adsorption from single protein solutions of fibrinogen, BSA, and complex mixed proteins are systematically investigated. A Pluronic F108 treated surface is highly resistant to nonspecific protein adsorption under the optimized conditions (MW of 15 k and PEO/PPO ratio of 80/20). This work demonstrates that the PEO-PPO-PEO polymer is able to achieve ultra low fouling surface via surface modification by controlling surface packing density of polymers (molecular weight, hydrophobic/hydrophilic ratio, and hydrophilic group coverage).

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Bioinert Surface of Pluronic-Immobilized Flask for Preservation of Hematopoietic Stem Cells

Cited by 19 publications

References 27 publications

Thermoresponsive hydrogels in biomedical applications

Thermoresponsive hydrogels in biomedical applications

Temperature Responsive Gels Based on Pluronic F127 and Poly(vinyl alcohol)

A systematic SPR study of human plasma protein adsorption behavior on the controlled surface packing of self‐assembled poly(ethylene oxide) triblock copolymer surfaces

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