Bioinformatic analyses reveal the prognostic significance and potential role of ankyrin 3 (ANK3) in kidney renal clear cell carcinoma

Abstract

Kidney renal clear cell carcinoma (KIRC) is one of the most aggressive cancer type of the urinary system. Metastatic KIRC patients have poor prognosis and limited therapeutic options. Ankyrin 3 (ANK3) is a scaffold protein that plays important roles in maintaining physiological function of the kidney and its alteration is implicated in many cancers. In this study, we investigated differential expression of ANK3 in KIRC using GEPIA2, UALCAN, and HPA databases. Survival analysis was performed by GEPIA2, Kaplan-Meier plotter, and OSkirc databases. Genetic alterations of ANK3 in KIRC were assessed using cBioPortal database. Interaction network and functional enrichment analyses of ANK3-correlated genes in KIRC were performed using GeneMANIA and Shiny GO, respectively. Finally, the TIMER2.0 database was used to assess correlation between ANK3 expression and immune infiltration in KIRC. We found that ANK3 expression was significantly decreased in KIRC compared to normal tissues. The KIRC patients with low ANK3 expression had poorer survival outcomes than those with high ANK3 expression. ANK3 mutations were found in 2.4% of KIRC patients and were frequently co-mutated with several genes with a prognostic significance. ANK3-correlated genes were significantly enriched in various biological processes, mainly involved in peroxisome proliferator-activated receptor (PPAR) signaling pathway, in which positive correlations of ANK3 with PPARA and PPARG expressions were confirmed. Expression of ANK3 in KIRC was significantly correlated with infiltration level of B cell, CD8+ T cell, macrophage, and neutrophil. These findings suggested that ANK3 could serve as a prognostic biomarker and promising therapeutic target for KIRC.

Analysis and Verification of Glycosylation Characteristics of Septic Acute Kidney Injury

“…ANK3 was distributed in the proximal and distal convoluted tubules of the kidney and was expressed in the renal tissues of patients with kidney cancer, and the abundance of ANK3 in kidney cancer tissues was positively correlated with the prognosis. 25 The gene encoding uromodulin illustrates how a kidney-specific protein of major physiological importance is involved in a spectrum of kidney disorders. 26 The in vitro knockdown of COL7A1 expression significantly affected ccRCC cell migration.…”

Identifying and validating MMP family members (MMP2, MMP9, MMP12, and MMP16) as therapeutic targets and biomarkers in kidney renal clear cell carcinoma (KIRC)

“…Kidney cancer, also known as renal cell carcinoma, is a serious and potentially life-threatening condition that affects millions of people worldwide [ 1 – 3 ]. The most common type of kidney cancer is clear cell renal carcinoma (KIRC), which accounts for approximately 70% of all cases [ 4 ]. KIRC arises from the lining of the small tubes in the kidney that filter blood and remove waste [ 5 ].…”