Bioinformatic analysis of antivirus‐related TRIM genes in Dabry’s sturgeon Acipenser dabryanus

Abstract

TRIM proteins are a group of highly conserved proteins that participate in a variety of biological processes in innate immunity. However, the roles of the Acipenseridae trim genes in the anti‐bacterial immune response remained uncertain. In the present study, the Dabry’s sturgeon (Acipenser dabryanus) TRIM2, TRIM8, and TRIM59 protein sequences were found to be similar to Chicken (Gallus gallus), with identities 92.8%, 89.1%, and 79.4%, respectively. Meanwhile, the TRIM23, TRIM37, and TRIM82 proteins shared 95.8%, 81.7%, and 78.1% similarity with Zebrafish (Danio rerio) homologs, respectively. Sequence alignment indicated that these six adTRIMs contained incomplete domains at their N‐termini, including a RING‐finger domain and one or two B‐boxes domains. The six adtrim genes were constitutively expressed in immune related tissues, such as the head kidney, and were also expressed in non‐immune related tissues, like the blood, brain, muscle, and liver. After challenge with Aeromonas hydrophila, the expression levels of adtrims changed during the early stage of infection. Stimulation by A. hydrophila upregulated the expression levels of adtrim8 and adtrim59 in the spleen and caudal kidney, while the expression of adtrim82 in the spleen and caudal kidney was inhibited at 12 hr post‐infection. A. hydrophila challenge led to significant upregulation of adtrim2 and downregulation of adtrim23 in the spleen. In addition, adtrim37 was only weakly affected by A. hydrophila stimulation. These results suggested that adtrims were related to the immune response to bacterial infection, which provides the fundamental basis for protecting Dabry’s sturgeon against pathogen attack.

The immune system of sturgeons and paddlefish (Acipenseriformes): a review with new data from a chromosome‐scale sturgeon genome

“…Although in sharks, sturgeon and teleosts a more complex receptor system is suggested than in mammals (Secombes & Zou 2017;Luo et al 2018b), all signalling pathways, downstream of IFNRs (interferon receptors) appear to be evolutionarily conserved between all these groups (Langevin et al 2013). Regulators, such as IRFs (interferon regulating factors) and TRIM (tripartite motif-containing) proteins are also described for these taxa (Ozato et al 2008;Secombes & Zou 2017;Li et al 2017c;Li et al 2019).…”

Characterization of two tripartite motif‐containing genes from Asian Seabass Lates calcarifer and their expression in response to virus infection and microbial molecular motifs

“…The LcTRIM21 was predominantly expressed in muscle, followed by gill, head kidney, and blood. Some other TRIM genes like Dabry's Sturgeon Acipenser dabryanus TRIM71 (Chen et al 2021), TRIM8 genes (Li et al 2019), and Zebrafish TRIM54 (Boudinot et al 2011) also showed higher expression in muscle. Moreover, TRIM21 was reported to be expressed in most histogenetic lineages than limiting expression to leukocytes (Vaysburd et al 2013).…”

Molecular characterization and expression analysis of double‐stranded RNA‐dependent protein kinase (PKR) in Dabry’s sturgeon ( Acipenser dabryanus )

“…In recent years, sturgeon farms have suffered high mortality rates due to various bacterial diseases, resulting in huge losses to sturgeon aquaculture and research (Luo et al., 2018; Meng et al., 2011; Zhang, Xu, Boscari, et al., 2018). Immune‐related proteins such as IFNs, IFN receptors, interleukin 16/17, trim, lysozymes, antimicrobial peptides, and Toll‐like receptors (TLRs) have been characterised (Han et al., 2018; Kai et al., 2019; Youshen Li et al., 2019; Luo et al., 2018; Xu et al., 2019; Zhang, Xu, Boscari, et al., 2018; Zhang, Xu, Du, et al., 2018). The interferon‐stimulating, Mx and Viperin genes have also been reported in A. dabryanus (Zhang et al., 2019).…”