Bioinformatic analysis of eosinophil activity and its implications for model and target species

Jenvey, Caitlin J.; Alenizi, Dalal; Almasi, Fazel; Cairns, Callum; Holmes, Andrew; Sloan, Sarah; Stear, M. J.

doi:10.1017/s0031182019001768

Cited by 7 publications

(5 citation statements)

References 96 publications

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“…The immune response of eosinophilia among induced infected sheep plays an important role to fight against the gastrointestinal nematodes, particularly H. contortus (Balic et al, 2006;Terefe et al, 2009). However, the mechanisms of eosinophil action were reported to vary between host species (Jenvey et al, 2020;Hayyan et al, 2020) and it is species-specific (Henderson et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Multiple Anthelmintic Resistance Among Dorper Sheep Detected With Phenotypic Markers Against Parasitic Gastroenteritis

MAJID

ISHAK

MURUGAPIRAN

et al. 2022

MAB

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Parasitic gastroenteritis (PGE) is a significant disease that affects small ruminant production. PGE is controlled exclusively by chemical anthelmintics but restricted by anthelmintic resistance. Hence, dependence on anthelmintics needs to be reduced. This study aimed to investigate the anthelmintic resistance status of a Dorper sheep farm while determining the phenotypic markers of resistance to PGE. Sheep that met the criteria of the Faecal Egg Count Reduction Test (FECRT) were divided into a control and four treatment groups of 11 to 13 animals per group. Faecal and blood samples at pre- and post-treatments were subjected to faecal egg counts (FEC), faecal culture, packed cell volume (PCV) and peripheral eosinophil counts (PEC). The data were analysed by Spearman rank correlation and two-way ANOVA. FECRT showed resistance towards albendazole, levamisole, fenbendazole and ivermectin which was predominated by Haemonchus contortus. Significant negative correlations were observed between FEC and PCV in control (r=-0.88, p<0.01), fenbendazole (r=-0.58, p<0.01) and ivermectin (r=-0.69, p<0.01) groups. Significant positive correlations were detected between FEC and PEC in control (r=0.95, p<0.01) and levamisole (r=0.56, p<0.01) groups. This study shows multiple anthelmintic resistance with promising resistant characteristics against PGE among sheep.

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Section: Discussionmentioning

confidence: 99%

Multiple Anthelmintic Resistance Among Dorper Sheep Detected With Phenotypic Markers Against Parasitic Gastroenteritis

MAJID

ISHAK

MURUGAPIRAN

et al. 2022

MAB

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“…As we saw in zebrafish for Embp, hMBP-2 is exclusively expressed in eosinophils 34 , while hMBP-1, although predominantly expressed in eosinophils is also expressed in basophils and mast cells, suggesting that embp may be the ortholog of hMBP-2. Interestingly, in ruminants, MBP-2 is the only MBP present 35 . Also, the relatively neutral pI of Embp suggests that the characteristically strongly basic pI of hMBP-1 may be a relatively recent evolutionary occurance.…”

Section: Discussionmentioning

confidence: 98%

“…Also, the relatively neutral pI of Embp suggests that the characteristically strongly basic pI of hMBP-1 may be a relatively recent evolutionary occurance. Indeed, the pIs of MBP orthologues vary considerably across species 34,35 . Although further studies into the biochemical and functional properties of this zebrafish orthologue will be needed, zebrafish embp will likely offer an interesting window into the evolution of MBP proteins.…”

Section: Discussionmentioning

confidence: 99%

Identification of a specific granular marker of zebrafish eosinophils enables development of new tools for their study

Herbert,

Goosmann,

Brinkmann

et al. 2024

Preprint

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Eosinophils control many aspects of the vertebrate innate immune response. They contribute to homeostasis, inflammatory conditions and defense against pathogens, yet, their function in disease often remains enigmatic. The zebrafish has emerged as a useful model organism for human diseases but tools to study eosinophils in this model are severely limited. Here, we characterize a new and highly specific marker for eosinophils in zebrafish and report a new transgenic reporter line to visualize eosinophils in vivo. In addition, we created a polyclonal antibody that allows the identification of eosinophils ex vivo. These new tools expand the toolkit to study eosinophils in the zebrafish model. Using our tools, we have been able to identify the likely ortholog of eosinophil major basic protein, a critical mediator of eosinophil function, in zebrafish. These advances will allow for the investigation of eosinophil biology in the zebrafish model organism, allowing researchers to identify the contribution of eosinophils to the many diseases that are modeled within zebrafish and also shed light on the evolution of eosinophils within vertebrates.

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“…White blood cell counts are directly related to the immune response of animals. Of the white blood cells, eosinophils are the most important cells and related to the protection and immune response against infections of gastrointestinal parasites (Jenvey et al 2020;Mitre and Klion 2021). Eosinophilia is a typical consequence of infection with helminths (Hendawy 2018).…”

Section: Discussionmentioning

confidence: 99%

Hematological and Performance Parameters Trend in Steers and Heifers in Feed Lot and Supplemented with Palm Oil (Elaeis guineensis Jack)

2023

Int J Vet Sci

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This study compares the daily weight gain (ADG) and the hematological profile of cattle in feed lot and supplemented with palm oil (POil). Overall, 20 male and 24 female Holstein-Cebu (5/8-3/8) cattle with an average weight of 249±43 kg were used. Three groups of eight animals each were formed with females, which were housed and received 0, 2, and 4% POil in the diet (FSWO, FS2O, FS4O). With males, only two groups were formed, one group that kept grazing and received 2% POil (MG2O, n=8) and one group stabled and received 4% POil (MS4O, n=12). Peripheral blood was obtained to determine the hematocrit (HCT), plasma protein concentration (TPP, g/dL), and eosinophil count (EOS), and a complete blood sample was processed in an automatic analyzer to determine the erythron and platelet formula. Analysis of repeated measures over time was performed. Stabled steers showed the highest ADG (1.5kg/d), whereas females received 2 or 4% oil had similar ADG levels (1.3 to 1.4kg/d) than grazing steers. At the first sampling, EOS counts were 1.5 x 103 cells/µL of blood, but they were reduced to 0.23 x 103 cells/µL of blood at the end of the study. The lowest HCT was obtained in grazing steers (32.8%). It is concluded that in stabled steers, the red formula is improved, and the EOS count reduced. Palm oil supplementation generated the highest daily weight gain in stabled steers.

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Bioinformatic analysis of eosinophil activity and its implications for model and target species

Cited by 7 publications

References 96 publications

Multiple Anthelmintic Resistance Among Dorper Sheep Detected With Phenotypic Markers Against Parasitic Gastroenteritis

Multiple Anthelmintic Resistance Among Dorper Sheep Detected With Phenotypic Markers Against Parasitic Gastroenteritis

Identification of a specific granular marker of zebrafish eosinophils enables development of new tools for their study

Hematological and Performance Parameters Trend in Steers and Heifers in Feed Lot and Supplemented with Palm Oil (Elaeis guineensis Jack)

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