Bioinformatic analysis of the flagellar system of the rickettsia-like alphaproteobacteria Candidatus Hepatobacter penaei

Leyva, Juan Manuel; Martínez‐Porchas, Marcel; Vargas‐Albores, Francisco; Hernández‐López, Jorge; Gollas‐Galván, Teresa

doi:10.4067/s0718-19572017000100010

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…Conversely, among other Holosporaceae , ‘ Ca . Hepatobacter’ possesses only a partial set (Leyva et al, 2017), devoid of filament and hook, and other members fully lack this organelle. Besides, we identified a gene set for chemotaxis in Bealeia , unique among Holosporaceae , as well as in the ‘ Ca .…”

Section: Resultsmentioning

confidence: 99%

Novel evolutionary insights on the interactions of the Holosporales (Alphaproteobacteria) with eukaryotic hosts from comparative genomics

Giovannini,

Petroni,

Castelli

2024

Environmental Microbiology

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Holosporales are an alphaproteobacterial order engaging in obligate and complex associations with eukaryotes, in particular protists. The functional and evolutionary features of those interactions are still largely undisclosed. Here, we sequenced the genomes of two members of the species Bealeia paramacronuclearis (Holosporales, Holosporaceae) intracellularly associated with the ciliate protist Paramecium, which resulted in high correspondence. Consistent with the short‐branched early‐divergent phylogenetic position, Bealeia presents a larger functional repertoire than other Holosporaceae, comparable to those of other Holosporales families, particularly for energy metabolism and motility. Our analyses indicate that different Holosporales likely experienced at least partly autonomous genome reduction and adaptation to host interactions, for example regarding dependence on host biotin driven by multiple independent horizontal acquisitions of transporters. Among Alphaproteobacteria, this is reminiscent of the convergently evolved Rickettsiales, which however appear more diverse, possibly due to a probably more ancient origin. We identified in Bealeia and other Holosporales the plasmid‐encoded putative genetic determinants of R‐bodies, which may be involved in a killer trait towards symbiont‐free hosts. While it is not clear whether these genes are ancestral or recently horizontally acquired, an intriguing and peculiar role of R‐bodies is suggested in the evolution of the interactions of multiple Holosporales with their hosts.

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Section: Resultsmentioning

confidence: 99%

Novel evolutionary insights on the interactions of the Holosporales (Alphaproteobacteria) with eukaryotic hosts from comparative genomics

Giovannini,

Petroni,

Castelli

2024

Environmental Microbiology

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“…Despite flagellar movement in bacteria of the Rickettsiaceae family has been reported (Vannini, Boscaro, & Ferrantini, ), flagellar motility is not common in an obligate intracellular organism. Holospora genomes do not contain entire flagellar apparatus, but NHP‐B seems to retain the function of its flagellar system, including motility (Leyva, Martínez‐Porchas, Vargas‐Albores, Hernández‐Lopez, & Gollas‐Galvan, ).…”

Section: Discussionmentioning

confidence: 99%

Identifying the causal agent of necrotizing hepatopancreatitis in shrimp: Multilocus sequence analysis approach

et al. 2018

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The causal agent of the necrotizing hepatopancreatitis (NHP) disease in penaeid shrimp and other crustaceans was studied by sequencing its genome and locating particular biomarker genes that could contribute to a better taxonomic classification. Hepatopancreas of shrimp with clear signs of NHP was used as infective inoculum. The disease was reproduced and the causal agent was isolated by Percoll gradient. Genomic DNA was extracted, purified, submitted to high throughput sequencing and de novo assembled. Thereafter, strategies aimed to improve the taxonomic classification of the pathogen were used. A phylogenetic classification by multilocus sequence analysis (MLSA) was performed using the complete gene sequences of 16S rRNA, gyrase subunit alpha (gyrA), gyrase subunit beta (gyrB), RNA polymerase subunit beta (rpoB), ATP synthase subunit beta (atpD) and recombinase subunit alpha (recA). Phylogenetic analysis confirmed with a greater degree of confidence that the causal agent of NHP in shrimp belongs to the Holosporaceae family, with similarity to some species considered as pathogens of other arthropods.

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Bioinformatic analysis of the flagellar system of the rickettsia-like alphaproteobacteria Candidatus Hepatobacter penaei

Cited by 2 publications

References 24 publications

Novel evolutionary insights on the interactions of the Holosporales (Alphaproteobacteria) with eukaryotic hosts from comparative genomics

Novel evolutionary insights on the interactions of the Holosporales (Alphaproteobacteria) with eukaryotic hosts from comparative genomics

Identifying the causal agent of necrotizing hepatopancreatitis in shrimp: Multilocus sequence analysis approach

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