Bioinformatics Analysis Discovers Microtubular Tubulin Beta 6 Class V (TUBB6) as a Potential Therapeutic Target in Glioblastoma

Abstract

Glioblastoma (GBM) has long been a major clinical research challenge to scientists. The pivotal role of the mitochondria related gene family in the promotion of GBM tumorigenesis is not clear. We detected that microtubular tubulin beta 6 class V (TUBB6) was one of 33 differentially expressed mitochondrial-focused genes (DEMFGs) in GBM, and considered that TUBB6 is a potential therapeutic target in GBM. TUBB6 was vital for GBM and marked as the key prognostic gene in primary GBM. Mutations of TUBB6 in GBM were rare. Only four TUBB6 co-expressed hub genes (ANXA2, S100A11, FLNA, and MSN) exhibited poorer overall survival rates in higher expression groups (p-value < 0.05). We have confirmed the up-regulation of TUBB6 and its partners, ANXA2 and S100A11 in GBM and validated their importance as prognostic factors in primary GBM. TUBB6 was significantly correlated with stromal score in GBM samples (p-value = 6.99E-04). This study aimed to assess the importance of novel hub genes by analyzing the expression, potential function and prognostic impact of TUBB6 in human primary GBM cancer.

A Fast and Efficient Approach to Obtaining High-Purity Glioma Stem Cell Culture

“…As expected, we observed higher expression of cell cycle genes and lower expression of p53 tumor suppressor gene expression in hGSCs than that in hNSCs. We have analyzed the 10 GBM-related genes reported recently (Jiang et al, 2020;Larsson et al, 2020;Yang et al, 2020) and six of them showed expression differences between hGSCs and hNSCs, further validating our hGSC derivation approach.…”

“…In contrast, the expression of the p53 tumor suppressor gene was lower in hGSCs than that in hNSCs ( Figure 5E ). IGFB2 and tubulin beta 6 class V (TUBB6), two potential therapeutic targets in GBM treatment ( Jiang et al, 2020 ), both displayed significantly lower expression in hGSCs than in hNSCs ( Figure 5F ).…”

“…DEG, multivariate Cox, and microarray data K-M curve analyses also identified SLC12A5 , CCL2 , IGFBP2 , and PDPN as independent predictors of survival in patients with GBM ( Yang et al, 2020 ). Differentially expressed mitochondrial-focused gene (DEMFG) analysis also suggested microtubular TUBB6 as a potential therapeutic target in GBM ( Jiang et al, 2020 ). Besides, Immunocore, an immune-infiltration-based signature, was also being considered as a potentially reliable prognostic and predictive tool for GBM ( Tang et al, 2020 ).…”

Expression characteristics of long noncoding RNA and messenger RNA in human traumatic brain injury

“…TUBB6 is a member of the beta tubulin superfamily, which is a major component of microtubules, and has a molecular weight of approximately 50 kDa [31]. TUBB6 contributes to many cellular processes, such as structural support, intracellular transport, and DNA segregation [32]. Nrf2 is a nuclear transcription factor, which plays an important role in TBI [33].…”

Machine learning for the micropeptide encoded by LINC02381 regulates ferroptosis through the glucose transporter SLC2A10 in glioblastoma

“…We have been working on basic research on gliomas and anti-tumor medicinal plants, including gene clusters, tumor markers [8], and noncoding RNA-based translational medicine research [9]. For example, we identified the potential role of SRY-Box Transcription Factor 6 (SOX6) in GBM [10]; and analyzed the gene set of mitochondrial metabolism-related genes and discovered the importance of Annexin A2 (ANXA2), S100 Calcium Binding Protein A11 (S100A11), and Tubulin Beta 6 Class V (TUBB6) as prognostic factors for GBM [11]. We found the function of sugar transporters (SWEETs) in camellia flower development in the early tea tree [12], while the sugar transporter homologous to humans and plants is Solute Carrier Family 50 Member 1 (SLC50A1).…”