Bioinformatics analysis of the wheel treadmill test on motor function recovery after spinal cord injury

Wang, Qiu‐Lin; Li, Tingting; Fang, Chang‐Le; Zhang, Bao‐Lei

doi:10.1002/ibra.12006

Cited by 2 publications

(3 citation statements)

References 47 publications

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“…no. 2002746; Illumina Inc.) with Illumina MiSeq (Illumina, Inc.), as previously described ( 32 ). miRDeep2 software ( github.com/rajewsky-lab/mirdeep2 ) was used to predict the novel miRNAs.…”

Section: Methodsmentioning

confidence: 99%

miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis

Nie

Zhou

et al. 2023

Exp Ther Med

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MicroRNAs (miRNAs or miRs) are commonly involved in regulating myocardial ischemia/reperfusion (I/R) injury by binding and silencing their target genes. However, whether miRNAs regulate myocardial I/R-induced pyroptosis remains unclear. The present study established an in vivo rat model of myocardial I/R injury and in vitro hypoxia/reoxygenation (H/R) injury model in rat primary cardiomyocytes to investigate the function and the underlying mechanisms of miRNAs on I/R injury-induced pyroptosis. RNA sequencing was utilized to select the candidate miRNAs between normal and I/R group. Reverse transcription-quantitative PCR and western blotting were performed to detect candidate miRNAs (miR-30c-5p, also known as miR-30c) and SRY-related high mobility group-box gene 9 (SOX9) expression, as well as expression of pyroptosis-associated proteins (NF-κB, ASC, caspase-1, NLRP3) in the myocardial I/R model. ELISA was used to measure pyroptosis-associated inflammatory markers IL-18 and IL-1β. Moreover, the link between miR-30c and SOX9 was predicted using bioinformatics and luciferase reporter assay. In myocardial I/R injured rats, miR-30c was downregulated, while the expression of SOX9 was upregulated. Overexpression of miR-30c inhibited pyroptosis both in vivo and in vitro . Furthermore, miR-30c negatively regulated SOX9 expression by binding its 3'untranslated region. In conclusion, the miR-30c/SOX9 axis decreased myocardial I/R injury by suppressing pyroptosis, which may be a potential therapeutic target.

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Section: Methodsmentioning

confidence: 99%

miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis

Nie

Zhou

et al. 2023

Exp Ther Med

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“…In addition, there are some other methods to treat SCI. The wheeled treadmill test shows the recovery of motor function after SCI 5 . Through three‐dimensional bioprinting, a series of spinal cord biometric scaffolds are being developed to improve motor function after SCI 6 .…”

Section: Introduction Of Spinal Cord Injurymentioning

confidence: 99%

“…The wheeled treadmill test shows the recovery of motor function after SCI. 5 Through three‐dimensional bioprinting, a series of spinal cord biometric scaffolds are being developed to improve motor function after SCI. 6 However, none of the above treatments can be used to completely treat SCI, and can only alleviate symptoms and complications.…”

Section: Introduction Of Spinal Cord Injurymentioning

confidence: 99%

Therapeutic effect of umbilical cord blood cells on spinal cord injury

Zhang

Steven

Liao

2023

Ibrain

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Spinal cord injury (SCI) is a nervous system disease characterized by sensory and motor dysfunction, axonal apoptosis, decreased vascular density, and inflammation. At present, surgical treatment, drug treatment, and cell therapy can be used. Surgical treatment can improve motor and independent function scores, and drug treatment can promote the recovery of neurons in the spinal cord, but only improve symptoms. Complete recovery of SCI has not yet been achieved. However, the differentiation of stem cells brings hope for the treatment of SCI. Umbilical cord blood cells (UCBs) are ethically readily available and can repair neuronal damage. However, it is still unclear how they can improve symptoms and repair nerve severity. In this paper, the role of UCBs in the treatment of SCI is described in detail from different aspects such as behavior, morphology, and molecular expression changes, so as to provide new ideas and theoretical directions for future research.

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Bioinformatics analysis of the wheel treadmill test on motor function recovery after spinal cord injury

Cited by 2 publications

References 47 publications

miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis

miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis

Therapeutic effect of umbilical cord blood cells on spinal cord injury

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