Bioinformatics Analysis of the Z-Carotene Desaturase Gene in Potato (Solanum Tuberosum)

Abstract

Abstract. Ζ-carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Solanum tuberosum ZDS (StZDS) gene sequences were obtained from Spud DB database, and preformed for bioinformatics analysis. The StZDS gene mapped to chromosomes 1, and contains an open reading frame of 1,767 bp that encodes a 588-amino acid protein with a calculated molecular mass of 64.70 kD and an isoelectric point (pI) of 8.6. Subcellular localization predicted the StZDS gene was in the chloroplast. The conserved domain of the StZDS protein is PLN02487. The ZDS protein is most closely related to Solanum lycopersicum. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in potato.