Bioinformatics Identification of Key Genes for the Development and Prognosis of Lung Adenocarcinoma

Abstract

Objective: Lung adenocarcinoma (LUAD) is a common malignant tumor with a poor prognosis. The present study aimed to screen the key genes involved in LUAD development and prognosis. Methods: The transcriptome data for 515 LUAD and 347 normal samples were downloaded from The Cancer Genome Atlas and Genotype Tissue Expression databases. The weighted gene co-expression network and differentially expressed genes were used to identify the central regulatory genes for the development of LUAD. Univariate Cox, LASSO, and multivariate Cox regression analyses were utilized to identify prognosis-related genes. Results: The top 10 central regulatory genes of LUAD included IL6, PECAM1, CDH5, VWF, THBS1, CAV1, TEK, HGF, SPP1, and ENG. Genes that have an impact on survival included PECAM1, HGF, SPP1, and ENG. The favorable prognosis genes included KDF1, ZNF691, DNASE2B, and ELAPOR1, while unfavorable prognosis genes included RPL22, ENO1, PCSK9, SNX7, and LCE5A. The areas under the receiver operating characteristic curves of the risk score model in the training and testing datasets were .78 and .758, respectively. Conclusion: Bioinformatics methods were used to identify genes involved in the development and prognosis of LUAD, which will provide a basis for further research on the treatment and prognosis of LUAD.

Comprehensive analysis and validation of SNX7 as a novel biomarker for the diagnosis, prognosis, and prediction of chemotherapy and immunotherapy response in hepatocellular carcinoma

“…It has also been found to be essential for fetal liver development in zebrafish and acts as an antiapoptotic protein in Hela or HepG2 cells [ 6 ]. Interestingly, recent studies have suggested that SNX7 may have potential value in clinical prediction of lung cancer [ 9 , 10 ], which is similar to our observations in HCC. Here, we analyzed and confirmed that both the mRNA and protein levels of SNX7 are significantly higher in HCC, which is strongly correlated with the pathologic stage of the disease and can serve as an independent risk factor for HCC patients.…”

“…For instance, one study developed a predictive model for recurrence in early-stage lung adenocarcinoma, incorporating SNX7 alongside other epithelial-to-mesenchymal transition-related genes (AGL, ECM1, ENPP1, SNX7, and TSPAN12) [ 9 ]. Another study identified SNX7 as an unfavorable prognosis gene in lung adenocarcinoma [ 10 ]. Nevertheless, the clinical value of its diagnosis, prognosis, and prediction of susceptibility to drug therapy in HCC has not been described.…”

Screening and Biological Function Analysis of miRNA and mRNA Related to Lung Adenocarcinoma Based on Bioinformatics Technology

“…Previous studies have shown that the biological processes of lung adenocarcinoma and glioma are mainly enriched in extracellular structural tissue, external encapsulated structural tissue, and extracellular matrix tissue [ 22 , 23 ]. Molecular functions in bladder cancer studies have been mainly concentrated in channel activity, passive transmembrane transporter activity, and gated channel activity [ 24 ].…”

Targeting proprotein convertase subtilisin/kexin type 9 (PCSK9): from bench to bedside

“… 339 In several studies, PCSK9 has been identified as a critical gene associated with unfavorable prognoses in lung adenocarcinoma. 334 , 350 , 351 Elevated PCSK9 expression has also been reported to be instrumental in promoting the metastasis and invasiveness of lung cancer and melanoma (specifically within the lung). 352 , 353 In addition, a clinical cohort study involving 44 elderly patients with non-small-cell lung cancer (NSCLC), who were treated with the ICI, nivolumab, revealed that NSCLC patients with lower levels of blood PCSK9 ( < 95 ng/mL) enjoyed a significantly prolonged OS during the second therapeutic cycle.…”