Bioinks and bioprinting technologies to make heterogeneous and biomimetic tissue constructs

Ashammakhi, Nureddin; Ahadian, Samad; Xu, Chun; Montazerian, Hossein; Ko, Hyojin; Nasiri, Rohollah; Barros, Natan Roberto de; Khademhosseini, Ali

doi:10.1016/j.mtbio.2019.100008

Cited by 374 publications

(349 citation statements)

References 256 publications

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“…Numerous nanocomposites are used to tune rheological properties of inks and a conclusive discussion is outside the scope of this paper. The interested reader is referred to a list of excellent papers and reviews covering this topic . However, in view of the recent developments for bioprinting a small number of examples is highlighted.…”

Section: Recent Progress For Controlling Shapementioning

confidence: 99%

“…During the last decade the biofabrication window has been extended by improving bioink performance and bioprinting techniques. Many excellent reviews discuss the current status of bioink development [16,[30][31][32][33][34] and this is beyond the scope of the current review; however, several relevant components for bioinks and the respective crosslinking approaches are listed in Table 1.…”

Section: Recent Progress For Controlling Shapementioning

confidence: 99%

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From Shape to Function: The Next Step in Bioprinting

et al. 2020

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In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell‐laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.

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Section: Recent Progress For Controlling Shapementioning

confidence: 99%

Section: Recent Progress For Controlling Shapementioning

confidence: 99%

From Shape to Function: The Next Step in Bioprinting

et al. 2020

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“…An important challenge in the 3D bioprinting technology remains the vascularization of constructs, that is the ability of building in vessels that are capable of anastomosing with host vessels following implantation [77]. With the aim to construct a stable vasculature, Gaebel and colleagues used a polyester urethane urea (PEUU) cardiac patch for bioprinting two types of human cells, ECs (4 × 10 6 cells) and MSCs (2 × 10 6 cells), in a defined arrangement mimicking the vasculature [78].…”

Section: Bioprinting Of Functional Myocardiummentioning

confidence: 99%

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

Gardin

Ferroni

Latrémouille

et al. 2020

Cells

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Three dimensional (3D) printing, which consists in the conversion of digital images into a 3D physical model, is a promising and versatile field that, over the last decade, has experienced a rapid development in medicine. Cardiovascular medicine, in particular, is one of the fastest growing area for medical 3D printing. In this review, we firstly describe the major steps and the most common technologies used in the 3D printing process, then we present current applications of 3D printing with relevance to the cardiovascular field. The technology is more frequently used for the creation of anatomical 3D models useful for teaching, training, and procedural planning of complex surgical cases, as well as for facilitating communication with patients and their families. However, the most attractive and novel application of 3D printing in the last years is bioprinting, which holds the great potential to solve the ever-increasing crisis of organ shortage. In this review, we then present some of the 3D bioprinting strategies used for fabricating fully functional cardiovascular tissues, including myocardium, heart tissue patches, and heart valves. The implications of 3D bioprinting in drug discovery, development, and delivery systems are also briefly discussed, in terms of in vitro cardiovascular drug toxicity. Finally, we describe some applications of 3D printing in the development and testing of cardiovascular medical devices, and the current regulatory frameworks that apply to manufacturing and commercialization of 3D printed products.Cells 2020, 9, 742 2 of 33 Cells 2020, 9, 742 3 of 33 in digital modeling softwares without the need for file type conversion [16]. Several medical image segmentation softwares are available; some of these are open-source and freely accessible, while others are commercial, licensed products. From the DICOM dataset, the target anatomic geometry is identified and segmented based on properties such as contrast or brightness [17]. As a result, segmentation masks are created such that pixels with the same intensity range are grouped and converted into 3D digital models using rendering techniques. These segmented digital models are then exported out in the standard tessellation language (STL) file type, which is the industry standard for 3D objects and 3D printing software [17]. Nevertheless, STL files do not contain color information and in order to print in multiple colors, a different file format will need to be used. For example, virtual reality modeling language (VRML) and additive manufacturing file format (AMF) provide multiple color and material options [18]. Once segmentation is completed, the final 3D digital model may be further modified within computer-aided design (CAD) software. The main post-processing adjustments of the 3D model are: Repairing errors and discontinuities that sometimes arise in the image segmentation and exporting processes, smoothing of the surface of the model due to scaling errors resulting from the resolution of the original medical image, and addition of other...

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“…As such, this technique can potentially substitute microfabrication techniques that require skilled technicians or cleanrooms . Furthermore, precise placement of bioinks with different chemical and mechanical properties at specific locations relative to each other enables the construction of complex 3D architectures similar to in vivo tissue microenvironment …”

Section: Design Consideration: How Simple Is Complex Enough?mentioning

confidence: 99%

“…[116] Furthermore, precise placement of bioinks with different chemical and mechanical properties at specific locations relative to each other enables the construction of complex 3D architectures similar to in vivo tissue microenvironment. [119] The 3D bioprinting techniques, such as extrusion-based, inkjet-based, and laser-based, can be grouped into direct and indirect printing methods. [118] The direct printing method comprises of printing constructs that cells will grow within.…”

Section: Microfabrication Techniquesmentioning

confidence: 99%

Recreating Physiological Environments In Vitro: Design Rules for Microfluidic‐Based Vascularized Tissue Constructs

Tan

Leung

2020

Small

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Vascularization of engineered tissue constructs remains one of the greatest unmet challenges to mimicking the native tissue microenvironment in vitro. The main obstacle is recapitulating the complexity of the physiological environment while providing simplicity in operation and manipulation of the model. Microfluidic technology has emerged as a promising tool that enables perfusion of the tissue constructs through engineered vasculatures and precise control of the vascular microenvironment cues in vitro. The tunable microenvironment includes i) biochemical cues such as coculture, supporting matrix, and growth factors and ii) engineering aspects such as vasculature engineering methods, fluid flow, and shear stress. In this systematic review, the design considerations of the microfluidic‐based in vitro model are discussed, with an emphasis on microenvironment control to enhance the development of next‐generation vascularized engineered tissues.

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Bioinks and bioprinting technologies to make heterogeneous and biomimetic tissue constructs

Cited by 374 publications

References 256 publications

From Shape to Function: The Next Step in Bioprinting

From Shape to Function: The Next Step in Bioprinting

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

Recreating Physiological Environments In Vitro: Design Rules for Microfluidic‐Based Vascularized Tissue Constructs

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