Bioinks for 3D bioprinting: an overview

Gungor-Ozkerim, P. Selcan; İnci, İlyas; Zhang, Yu Shrike; Khademhosseini, Ali; Dokmeci, Mehmet R.

doi:10.1039/c7bm00765e

Cited by 989 publications

(773 citation statements)

References 148 publications

(202 reference statements)

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“…Hydrogels' use has increased over the past 30 years in biomedical applications such as drug delivery, bioinks for 3D printing, and cell therapies . Hydrogels are readily suited for biological applications because of their tissue‐like mechanical properties, high water content, biocompatibility, and stimuli‐responsiveness.…”

Section: Rheological Properties Of Hpmc:np and Alginate Materialsmentioning

confidence: 99%

Non‐Newtonian Polymer–Nanoparticle Hydrogels Enhance Cell Viability during Injection

Hernandez

Grosskopf

Stapleton

et al. 2018

Macromolecular Bioscience

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Drug delivery and cell transplantation require minimally invasive deployment strategies such as injection through clinically relevant high‐gauge needles. Supramolecular hydrogels comprising dodecyl‐modified hydroxypropylmethylcellulose and poly(ethylene glycol)‐block‐poly(lactic acid) have been previously demonstrated for the delivery of drugs and proteins. Here, it is demonstrated that the rheological properties of these hydrogels allow for facile injectability, an increase of cell viability after injection when compared to cell viabilities of cells injected in phosphate‐buffered saline, and homogeneous cell suspensions that do not settle. These hydrogels are injected at 1 mL min−1 with pressures less than 400 kPa, despite the solid‐like properties of the gel when at rest. The cell viabilities immediately after injection are greater than 86% for adult human dermal fibroblasts, human umbilical vein cells, smooth muscle cells, and human mesenchymal stem cells. Cells are shown to remain suspended and proliferate in the hydrogel at the same rate as observed in cell media. The work expands on the versatility of these hydrogels and lays a foundation for the codelivery of drugs, proteins, and cells.

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Section: Rheological Properties Of Hpmc:np and Alginate Materialsmentioning

confidence: 99%

Non‐Newtonian Polymer–Nanoparticle Hydrogels Enhance Cell Viability during Injection

Hernandez

Grosskopf

Stapleton

et al. 2018

Macromolecular Bioscience

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“…Depending on the type or printing modality, specific concerns (e.g., shear stress, temperature, and chemical reaction‐based cell damage), arise based on deposition methodology and the specific biomaterial used; as crosslinking, curing kinetics, and chemical properties of the material will directly influence the printing process (Skardal & Atala, ). The use of natural polymers such as fibrin, alginate, and gelatin as bioinks have been demonstrated previously that can be used as excellent biomaterials for 3D inkjet printing (Gungor‐Ozkerim, Inci, Zhang, Khademhosseini, & Dokmeci, ).…”

Section: Inkjet 3d Bioprintingmentioning

confidence: 99%

Engineering inkjet bioprinting processes toward translational therapies

Angelopoulos

Allenby

Lim³

et al. 2019

Biotech & Bioengineering

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Bioprinting is the assembly of three‐dimensional (3D) tissue constructs by layering cell‐laden biomaterials using additive manufacturing techniques, offering great potential for tissue engineering and regenerative medicine. Such a process can be performed with high resolution and control by personalized or commercially available inkjet printers. However, bioprinting's clinical translation is significantly limited due to process engineering challenges. Upstream challenges include synthesis, cellular incorporation, and functionalization of “bioinks,” and extrusion of print geometries. Downstream challenges address sterilization, culture, implantation, and degradation. In the long run, bioinks must provide a microenvironment to support cell growth, development, and maturation and must interact and integrate with the surrounding tissues after implantation. Additionally, a robust, scaleable manufacturing process must pass regulatory scrutiny from regulatory bodies such as U.S. Food and Drug Administration, European Medicines Agency, or Australian Therapeutic Goods Administration for bioprinting to have a real clinical impact. In this review, recent advances in inkjet‐based 3D bioprinting will be presented, emphasizing on biomaterials available, their properties, and the process to generate bioprinted constructs with application in medicine. Current challenges and the future path of bioprinting and bioinks will be addressed, with emphasis in mass production aspects and the regulatory framework bioink‐based products must comply to translate this technology from the bench to the clinic.

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“…Various types of natural and synthetic polymers are used as bioinks. Natural ECM components such as collagen, gelatin, fibrin, hyaluronic acid, and synthetic polymers such as poly(ethylene glycol), polyethylene oxide, pluronic F127, and many others have been used as a bioink material (Gungor‐Ozkerim, Inci, Zhang, Khademhosseini, & Dokmeci, ; Irvine & Venkatraman, ). Also, few commercial bioinks have been introduced recently.…”

Section: Materials Used For Stem Cell Printingmentioning

confidence: 99%

Recent advances in three‐dimensional bioprinting of stem cells

Eswaramoorthy

Ramakrishna

Rath

2019

J Tissue Eng Regen Med

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In spite of being a new field, three‐dimensional (3D) bioprinting has undergone rapid growth in the recent years. Bioprinting methods offer a unique opportunity for stem cell distribution, positioning, and differentiation at the microscale to make the differentiated architecture of any tissue while maintaining precision and control over the cellular microenvironment. Bioprinting introduces a wide array of approaches to modify stem cell fate. This review discusses these methodologies of 3D bioprinting stem cells. Fabricating a fully operational tissue or organ construct with a long life will be the most significant challenge of 3D bioprinting. Once this is achieved, a whole human organ can be fabricated for the defect place at the site of surgery.

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Bioinks for 3D bioprinting: an overview

Cited by 989 publications

References 148 publications

Non‐Newtonian Polymer–Nanoparticle Hydrogels Enhance Cell Viability during Injection

Non‐Newtonian Polymer–Nanoparticle Hydrogels Enhance Cell Viability during Injection

Engineering inkjet bioprinting processes toward translational therapies

Recent advances in three‐dimensional bioprinting of stem cells

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