Bioinorganic Chemistry of Nickel

Maroney, Michael J.; Ciurli, Stefano

doi:10.3390/inorganics7110131

Cited by 6 publications

(2 citation statements)

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“…Nickel is an essential element for unicellular organisms and plants, being responsible for the catalytic activity of several enzymes, many involved in bacterial pathogenesis [ 92 ], and being tightly regulated intracellularly [ 93 ]. For humans, nickel is considered a dangerous metal ion, responsible of several pathologies such as immunotoxicity and cancer [ 94 ].…”

Section: Discussionmentioning

confidence: 99%

The Ni(II)-Binding Activity of the Intrinsically Disordered Region of Human NDRG1, a Protein Involved in Cancer Development

et al. 2022

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Nickel exposition is associated with tumors of the respiratory tract such as lung and nasal cancers, acting through still-uncharacterized mechanisms. Understanding the molecular basis of nickel-induced carcinogenesis requires unraveling the mode and the effects of Ni(II) binding to its intracellular targets. A possible Ni(II)-binding protein and a potential focus for cancer treatment is hNDRG1, a protein induced by Ni(II) through the hypoxia response pathway, whose expression correlates with higher cancer aggressiveness and resistance to chemotherapy in lung tissue. The protein sequence contains a unique C-terminal sequence of 83 residues (hNDRG1*C), featuring a three-times-repeated decapeptide, involved in metal binding, lipid interaction and post-translational phosphorylation. In the present work, the biochemical and biophysical characterization of unmodified hNDRG1*C was performed. Bioinformatic analysis assigned it to the family of the intrinsically disordered regions and the absence of secondary and tertiary structure was experimentally proven by circular dichroism and NMR. Isothermal titration calorimetry revealed the occurrence of a Ni(II)-binding event with micromolar affinity. Detailed information on the Ni(II)-binding site and on the residues involved was obtained in an extensive NMR study, revealing an octahedral paramagnetic metal coordination that does not cause any major change of the protein backbone, which is coherent with CD analysis. hNDRG1*C was found in a monomeric form by light-scattering experiments, while the full-length hNDRG1 monomer was found in equilibrium between the dimer and tetramer, both in solution and in human cell lines. The results are the first essential step for understanding the cellular function of hNDRG1*C at the molecular level, with potential future applications to clarify its role and the role of Ni(II) in cancer development.

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Section: Discussionmentioning

confidence: 99%

The Ni(II)-Binding Activity of the Intrinsically Disordered Region of Human NDRG1, a Protein Involved in Cancer Development

et al. 2022

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“…The higher levels of Ni were recorded mainly in honey samples from Szczecin and its vicinity, as well as Gorzów Wlkp., whereas the concentration was below the detection limit in those for Warsaw (Figure 2). The low concentration levels of Ni in the analysed honey samples showed no effect on their quality since the toxicity of Ni at low concentrations is debatable [61,62]. Apart from Ni, it was found that Cr, Co, and Ba were also present at low concentrations in the analysed honey samples (Figure 2).…”

Section: The Assessment Of Mms Concentration In Honey Samples From Nw and Central Poland And Other Eu Countriesmentioning

confidence: 93%

Micro and Macroelements in Honey and Atmospheric Pollution (NW and Central Poland)

et al. 2021

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Urban vegetation is generally exposed to high levels of air pollution in airborne particles, with the greatest exposure in the EU being seen in Poland. With the continuing growth of urban populations, there is a need to confirm whether honey produced from urban areas is of similar high quality to that from rural areas. A total of 27 honey samples were collected from urban and rural apiaries and tested for the concentrations of 19 elements by ICP-OES. The results were compared with data on honey produced in old and new EU countries (metadata). Our evaluation used a novel approach to determine threshold values in the identification of the bioproduct contamination index. The analysed urban honey samples demonstrated higher concentrations of K, Sr, Ba, Ni, and Co, and lower levels of Mn and B than rural honey samples. Contamination by PM10 particles and the toxic elements contained in them proved to be a poor predictor of the content of these elements in honey, in contrast to the effect of atmospheric pollution measured during firework shows, which demonstrated higher concentrations of Ba, Pb, Ca, Cu, and Mg. The non-carcinogenic risk assessment indicated that the analysed honey samples are of good quality and are comparable or of even better quality than honey products from other EU countries.

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EPR and optical studies of Ni(II) ions in Zn(4-chloropyrazole)6(ClO4)2 at 300 K: Probing the structure, bonding, and electron spin relaxation

Kathirvelu

2021

Journal of Physics and Chemistry of Solids

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Bioinorganic Chemistry of Nickel

Abstract: Following the discovery of the first specific and essential role of nickel in biology in 1975 (the dinuclear active site of the enzyme urease) [...]

Cited by 6 publications

References 13 publications

The Ni(II)-Binding Activity of the Intrinsically Disordered Region of Human NDRG1, a Protein Involved in Cancer Development

The Ni(II)-Binding Activity of the Intrinsically Disordered Region of Human NDRG1, a Protein Involved in Cancer Development

Micro and Macroelements in Honey and Atmospheric Pollution (NW and Central Poland)

EPR and optical studies of Ni(II) ions in Zn(4-chloropyrazole)6(ClO4)2 at 300 K: Probing the structure, bonding, and electron spin relaxation

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