Bioinspired antibacterial PVA/Melanin-TiO2 hybrid nanoparticles: the role of poly-vinyl-alcohol on their self-assembly and biocide activity

Pota, Giulio; Cavasso, Domenico; Varcamonti, Mario; D’Errico, Gerardino; Pezzella, Alessandro; Luciani, Giuseppina; Vitiello, Giuseppe

doi:10.1016/j.colsurfb.2021.111671

Cited by 24 publications

(11 citation statements)

References 46 publications

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“…As shown by the XRD spectra in Figure 1 , both Mel/TiO 2 and Mel/ZnO nanoparticles presented a crystalline structure, typical of anatase and wurtzite phases, respectively. The spectra agree with those previously obtained for both kinds of hybrid nanoparticles [ 37 , 38 ], thus confirming the formation and growth of the inorganic fraction of nanoparticles.…”

Section: Resultssupporting

confidence: 89%

“…Hybrid melanin-TiO 2 : Mel/TiO 2 nanoparticles were prepared through a synthesis methodology based on a hydrothermal route, as previously proposed and described by some authors of this work [ 36 , 37 , 38 ]. In particular, 3 mL of titanium isopropoxide (TTiP)/isopropanol solution (3.38 M in TTiP) was added dropwise to 15.65 mL of a water/acetic acid solution at pH 1.5.…”

Section: Methodsmentioning

confidence: 99%

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Nanoparticle Coatings on Glass Surfaces to Prevent Pseudomonas fluorescens AR 11 Biofilm Formation

et al. 2023

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Microbial colonization of surfaces is a sanitary and industrial issue for many applications, leading to product contamination and human infections. When microorganisms closely interact with a surface, they start to produce an exo-polysaccaridic matrix to adhere to and protect themselves from adverse environmental conditions. This type of structure is called a biofilm. The aim of our work is to investigate novel technologies able to prevent biofilm formation by surface coatings. We coated glass surfaces with melanin-ZnO2, melanin-TiO2, and TiO2 hybrid nanoparticles. The functionalization was performed using cold plasma to activate glass-substrate-coated surfaces, that were characterized by performing water and soybean oil wetting tests. A quantitative characterization of the antibiofilm properties was done using Pseudomonas fluorescens AR 11 as a model organism. Biofilm morphologies were observed using confocal laser scanning microscopy and image analysis techniques were used to obtain quantitative morphological parameters. The results highlight the efficacy of the proposed surface coating to prevent biofilm formation. Melanin-TiO2 proved to be the most efficient among the particles investigated. Our results can be a valuable support for future implementation of the technique proposed here in an extended range of applications that may include further testing on other strains and other support materials.

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Section: Resultssupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Nanoparticle Coatings on Glass Surfaces to Prevent Pseudomonas fluorescens AR 11 Biofilm Formation

et al. 2023

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“…A similar trend was observed following spontaneous oxidation of DA (10 mM) in droplets compared to bulk Dex (SI Figure S11d). Previous reports show that water-soluble polymers can help improving the colloidal stability of synthetic melanin or melanin-TiO 2 . While this approach also results in water-soluble melanin, it is fundamentally different from the system presented here, which relies on compartmentalization of melanin synthesis and accumulation of the melanin product in liquid droplets.…”

Section: Resultsmentioning

confidence: 66%

“…Previous reports show that water-soluble polymers can help improving the colloidal stability of synthetic melanin or melanin-TiO 2 . 36 While this approach also results in water-soluble melanin, it is fundamentally different from the system presented here, which relies on compartmentalization of melanin synthesis and accumulation of the melanin product in liquid droplets. Next, we evaluated how varying the irradiation time, and in turn ONB-Y cleavage, influence the formation of melanin product in the droplets.…”

Section: ■ Results and Discussionmentioning

confidence: 89%

Spatiotemporal Control of Melanin Synthesis in Liquid Droplets

Massarano

Leshem

Weitman

et al. 2022

ACS Appl. Mater. Interfaces

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Melanins are natural biopolymers that have remarkable properties including UV-protection, coloration, and antioxidant activity. Their biosynthesis is regulated both spatially and temporally and involves supramolecular templating and compartmentalization of enzymes and reactants within specialized organelles called melanosomes. In contrast, the laboratory-based bulk synthesis of melanin by tyrosine or dopamine oxidation is a poorly controlled process, resulting in materials with undefined properties. Inspired by the pigment’s biosynthesis, we developed a methodology to spatiotemporally regulate melanin formation in liquid droplets. The spatial control is achieved by sequestration of the reaction in dextran-rich droplets of a polyethylene glycol/dextran aqueous two-phase system, where the use of a photocleavable protected tyrosine provides a temporal control over its enzymatic oxidation–polymerization. We show that the liquid droplets allow for confined local reactivity as they serve as reaction centers for melanin synthesis and compartmentalize the melanin product. This methodology opens tremendous opportunities for applications in skincare and biomedicine.

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“… ,, Experimental ,,− and theoretical studies ,− focused on dopamine-functionalized TiO 2 also reported peculiar properties, such as a favorable binding of DNA, proteins, peptides, and other biomolecules and a surface enhanced Raman scattering (SERS) effect . Moreover, dopamine and its derivatives can polymerize on TiO 2 nanoparticles in a range of conditions: for example, oxides coupled with polydopamine show promising photocatalytic performances, − while the in situ polymerization of l -DOPA (3,4-dihydroxyphenylalanine) and DHICA (dihydroxyindole carboxylic acid) produces TiO 2 -melanin hybrids with antimicrobial activity. − …”

Section: Introductionmentioning

confidence: 99%

Interfacial Charge Transfer Complexes in TiO₂-Enediol Hybrids Synthesized by Sol–Gel

et al. 2022

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Metal oxide-organic hybrid semiconductors exhibit specific properties depending not only on their composition but also on the synthesis procedure, and particularly on the functionalization method, determining the interaction between the two components. Surface adsorption is the most common way to prepare organic-modified metal oxides. Here a simple sol−gel route is described as an alternative, finely controlled strategy to synthesize titanium oxide-based materials containing organic molecules coordinated to the metal. The effect of the molecular structure of the ligands on the surface properties of the hybrids is studied using three enediols able to form charge transfer complexes: catechol, dopamine, and ascorbic acid. For each system, the process conditions driving the transition from the sol to chemical, physical, or particulate gels are explored. The structural, optical, and photoelectrochemical characterization of the amorphous hybrid materials shows analogies and differences related to the organic component. In particular, electron paramagnetic resonance (EPR) spectroscopy at room temperature reveals the presence of organic radical species with different evolution and stability, and photocurrent measurements prove the effective photosensitization of TiO 2 in the visible range induced by interfacial ligand-to-metal charge transfer.

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Bioinspired antibacterial PVA/Melanin-TiO2 hybrid nanoparticles: the role of poly-vinyl-alcohol on their self-assembly and biocide activity

Cited by 24 publications

References 46 publications

Nanoparticle Coatings on Glass Surfaces to Prevent Pseudomonas fluorescens AR 11 Biofilm Formation

Nanoparticle Coatings on Glass Surfaces to Prevent Pseudomonas fluorescens AR 11 Biofilm Formation

Spatiotemporal Control of Melanin Synthesis in Liquid Droplets

Interfacial Charge Transfer Complexes in TiO₂-Enediol Hybrids Synthesized by Sol–Gel

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Bioinspired antibacterial PVA/Melanin-TiO2 hybrid nanoparticles: the role of poly-vinyl-alcohol on their self-assembly and biocide activity

Cited by 24 publications

References 46 publications

Nanoparticle Coatings on Glass Surfaces to Prevent Pseudomonas fluorescens AR 11 Biofilm Formation

Nanoparticle Coatings on Glass Surfaces to Prevent Pseudomonas fluorescens AR 11 Biofilm Formation

Spatiotemporal Control of Melanin Synthesis in Liquid Droplets

Interfacial Charge Transfer Complexes in TiO2-Enediol Hybrids Synthesized by Sol–Gel

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Product

Resources

About

Interfacial Charge Transfer Complexes in TiO₂-Enediol Hybrids Synthesized by Sol–Gel