Bioinspired Fabrication of Calcium-Doped TiP Coating with Nanofibrous Microstructure to Accelerate Osseointegration

Cai, Bianyun; Tan, Peijie; Jiang, Nan; Guo, Zhijun; Ay, Birol; Li, Shujun; Hou, Yi; Li, Yubao; You, Yonghua; Zhang, Li

doi:10.1021/acs.bioconjchem.0c00201

Cited by 11 publications

(9 citation statements)

References 64 publications

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“…ALN is recognized to be an effective anti-osteoporosis drug which can promote proliferation and osteogenic differentiation of osteoblasts and inhibit maturation of osteoclasts by disrupting the mevalonate pathway [ 24 , 48 , 55 , 56 ]. A sustained release of Ca 2+ can ameliorate proliferation and osteogenic differentiation of osteoblasts as reported previously [ 44 , 57 ].…”

Section: Resultssupporting

confidence: 62%

A programmed surface on polyetheretherketone for sequentially dictating osteoimmunomodulation and bone regeneration to achieve ameliorative osseointegration under osteoporotic conditions

Gao

Bai

Liao

et al. 2022

Bioactive Materials

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Section: Resultssupporting

confidence: 62%

A programmed surface on polyetheretherketone for sequentially dictating osteoimmunomodulation and bone regeneration to achieve ameliorative osseointegration under osteoporotic conditions

Gao

Bai

Liao

et al. 2022

Bioactive Materials

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“…Cai et al 89 hydrothermal treatment method (Figure 8B). For step one, the Ti sample was immersed in an aqueous solution (m(H PO 4 ):m(H 2 O ) = 1:9) at 220 °C for 24 hours to form TiP.…”

Section: Hydrothermal Treatmentmentioning

confidence: 99%

“…Figure 8. Use of hydrothermal treatment on Ti implants to fabricate (A) three unique micro-/nanostructured surface coatings involving TiP, and (B) bioinspired nanofibrous Ca-doped TiP coating 89. Reprinted with permission.…”

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confidence: 99%

Advances in implant surface modifications to improve osseointegration

Zhu

Wang

2021

Mater. Adv.

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“…The Ti scaffolds were subsequently subjected to hydrothermal treatment. [36] Specifically, the scaffolds were immersed in an aqueous solution (m(H 2 O 2 ):m(H 3 PO 4 ) 9:1) and placed in a Teflon-lined autoclave under 220 ℃ for 24 h. After the treatment, scaffolds were washed with distilled water and dried in air at room temperature for 24 h. The hydrothermally treated samples were named as HR-Ti.…”

Section: Preparation Of 3d Printed Ti6al4v Scaffoldsmentioning

confidence: 99%

3D Printed Multi Functional Ti6Al4V Based Hybrid Scaffold for the Management of Osteosarcoma

Cai¹,

Huang²,

Wang³

et al. 2021

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2.1.1 Preparation of drug-laden Gel/HA composite Alendronate (ALN), is a commonly used bisphosphonate drug for osteoporosis management and bone regeneration. [33] However, direct loading of ALN onto Gel often results in burst drug release due to the fast Gel degradation in vivo. In contrast, degradable HA microsphere is an excellent drug delivery vehicle, which can provide sustained/controlled drug release as well as promoting osteoblasts mineralization/maturation (due to slow release of Ca2+ and PO43-). [34] To prepare the ALN-laden HA microspheres/Gel composite (ALN-HA/Gel), HA microspheres were first synthesized following established procedure. [35] 0.5 g ALN was then added into 50 mL aqueous dispersion containing 2.0 g HA microspheres. After mixing using shaking incubator under 37 ℃ for 48 h, ALN-HA were collected by centrifugation and vacuum dried at 40 ℃ for 24 h. ALN concentration in the supernatant solution was evaluated by UV-vis spectroscopy. The drug-laden Gel/ALN-HA can be obtained by dispersing 3.0g ALN-HA microspheres in 50 mL deionized water, followed by addition of 8.0 g Gel, and stirred magnetically at 1000 rpm under 50 ℃ for 1 h. 2.1.2 Preparation of 3D printed Ti6Al4V scaffolds 3D printed Ti6Al4V scaffolds (named as Ti) were manufactured by electron beam melting (EBM) facility in-house. The lattice structure was designed by computer-assisted design (CAD) software (Magics, Materialise, Belgium) based on a dodecahedron unit cell with strut diameter of 300 μm and porosity of 80%. Cubic scaffolds (5mm × 5mm × 5mm) were used for in vitro testing and cylindrical scaffolds (Φ = 6mm, height = 6mm) were used for in vivo experiments. All samples were thoroughly cleaned by sonication in acetone, alcohol and deionized water for 30 min, respectively. The Ti scaffolds were subsequently subjected to hydrothermal treatment. [36] Specifically, the scaffolds were immersed in an aqueous solution (m(H2O2):m(H3PO4) 9:1) and placed in a Teflon-lined autoclave under 220 ℃ for 24 h. After the treatment, scaffolds were washed with distilled water and dried in air at room temperature for 24 h. The hydrothermally treated samples were named as HR-Ti. 2.1.3 Preparation of Gel/ALN-HA infused HR-Ti scaffold The Gel/ALN-HA mixture was infused into the HR-Ti, then the infused scaffolds were held at -80<a> </a>℃ overnight followed by freeze-drying at -40 ℃ for 48 h. After that, the infused scaffolds were immersed in 100 mL ethanol containing 50mmol/L 1-ethyl-3-(3- (dimethylamino) propyl) carbodiimide hydrochlorid (EDC∙HCl) and N-hydroxysuccinimide (NHS) at 4 ℃ for 10 h to crosslink the Gel content. Afterwards, the scaffolds were washed three times by ethanol and freeze-dried again. The final scaffolds were named as Gel/ALN-HA/HR-Ti. For comparison, ALN/HR-Ti (without Gel and HA), Gel/ALN/HR-Ti (without HA) and Gel/HA/HR-Ti (without ALN) were also prepared.

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Bioinspired Fabrication of Calcium-Doped TiP Coating with Nanofibrous Microstructure to Accelerate Osseointegration

Cited by 11 publications

References 64 publications

A programmed surface on polyetheretherketone for sequentially dictating osteoimmunomodulation and bone regeneration to achieve ameliorative osseointegration under osteoporotic conditions

A programmed surface on polyetheretherketone for sequentially dictating osteoimmunomodulation and bone regeneration to achieve ameliorative osseointegration under osteoporotic conditions

Advances in implant surface modifications to improve osseointegration

3D Printed Multi Functional Ti6Al4V Based Hybrid Scaffold for the Management of Osteosarcoma

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