Bioinspired Photonic Barcodes with Graphene Oxide Encapsulation for Multiplexed MicroRNA Quantification

Abstract

Multiplexed microRNA (miRNA) quantification has a demonstrated value in clinical diagnosis. In this paper, novel mussel-inspired photonic crystal (PhC) barcodes with graphene oxide (GO) encapsulation for multiplexed miRNA detection are presented. Using the excellent adhesion capability of polydopamine, the dispersed GO particles can be immobilized on the surfaces of the PhC barcodes to form an additional functional layer. The GO-decorated PhC barcodes have constant characteristic reflection peaks because the GO immobilization process not only maintains their periodic microstructure but also enhances their stability and anti-incoherent lightscattering capability. The immobilized GO particles are shown to enable high-sensitivity miRNA screening on the surface of the PhC barcodes by integration with a hybridization chain reaction amplification strategy. Because the PhC barcodes have stable encoding reflection peaks, multiplexed low-abundance miRNA quantification can also be achieved rapidly, accurately, and reproducibly by employing different GO-decorated PhC barcodes. These features should make GO-encapsulated PhC barcodes ideal for many practical applications.

Label-Free Quantifications of Multiplexed Mycotoxins by G-Quadruplex Based on Photonic Barcodes

“…The characteristic reflection peak position of colloidal crystal barcodes which consist of different size silica nanoparticles could be calculated according to Bragg's equation: λ = 1.633dn average , 29,37 where λ represents the wavelength peak of colloidal crystal barcodes, the diameter of adjacent SiO 2 nanoparticles is represented by d, and n average is on behalf of the average refractive index of the colloidal crystal ( Figure 2d). 31,37 The constitutes of colloidal crystal keep unchanged, n average is unchanged, and λ is only related to d. Hence, these characteristics demonstrate that a series of various reflection wavelength could be obtained by adjusting diameter of silica nanoparticles. Besides, the microstructure of the colloidal crystal barcodes was recorded by scanning electron microscopy (SEM), and the barcodes have a uniform diameter size of silica nanoparticles is 200 ± 10 nm and formed an ordered hexagonal alignment (Figure 2e,f).…”

Bio-Inspired Self-Adhesive Bright Non-iridescent Graphene Pigments

“…demonstrated the integration of mussels (i.e., DA) into functional materials for achieving adhesive features. [22][23][24][25] Thus, when the structural-color materials are combined with DA, they are imparted with good adhesion. Furthermore, feathers of many kinds of birds often display vivid and bright structural colors.…”

Colloidal Crystals from Microfluidics

“…Bian et al prepared colloidal crystal barcodes by microfluidics and modified them for quantification of miRNA ( Figure a) . The calcined colloidal crystal barcodes were incubated in Tris buffer (pH = 8.5) containing dopamine to form a polydopamine functional layer by self‐polymerization on the surface of the colloidal crystal barcodes.…”